RNF14 (NM_183399) Human Tagged ORF Clone

CAT#: RC223491

RNF14 (Myc-DDK-tagged)-Human ring finger protein 14 (RNF14), transcript variant 3

Product Images

Coomassie blue staining of purified RNF14 protein (Cat# TP323491). The protein was produced from HEK293T cells transfected with RNF14 cDNA clone (Cat# RC223491) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF14
• Synonyms: ARA54; HFB30; HRIHFB2038; TRIAD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223491 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGTCGTCAGAAGATCGAGAAGCTCAGGAGGATGAATTGCTGGCCCTGGCAAGTATTTACGATGGAGA
  TGAATTTAGAAAAGCAGAGTCTGTCCAAGGTGGAGAAACCAGGATCTATTTGGATTTGCCACAGAATTTC
  AAGATATTTGTGAGCGGCAATTCAAATGAGTGTCTCCAGAATAGTGGCTTTGAATACACCATTTGCTTTC
  TGCCTCCACTTGTGCTGAACTTTGAACTGCCACCAGATTATCCATCCTCTTCCCCACCTTCATTCACACT
  TAGTGGCAAATGGCTGTCACCAACTCAGCTATCTGCTCTATGCAAGCACTTAGACAACCTATGGGAAGAA
  CACCGTGGCAGCGTGGTCCTGTTTGCCTGGATGCAATTTCTTAAGGAAGAGACCCTAGCATACTTGAATA
  TTGTCTCTCCTTTTGAGCTCAAGATTGGTTCTCAGAAAAAAGTGCAGAGAAGGACAGCTCAAGCTTCTCC
  CAACACAGAGCTAGATTTTGGAGGAGCTGCTGGATCTGATGTAGACCAAGAGGAAATTGTGGATGAGAGA
  GCAGTGCAGGATGTGGAATCACTGTCAAATCTGATCCAGGAAATCTTGGACTTTGATCAAGCTCAGCAGA
  TAAAATGCTTTAATAGTAAATTGTTCCTGTGCAGTATCTGTTTCTGTGAGAAGCTGGGTAGTGAATGCAT
  GTACTTCTTGGAGTGCAGGCATGTGTACTGCAAAGCCTGTCTGAAGGACTACTTTGAAATCCAGATCAGA
  GATGGCCAGGTTCAATGCCTCAACTGCCCAGAACCAAAGTGCCCTTCGGTGGCCACTCCTGGTCAGGTCA
  AAGAGTTAGTGGAAGCAGAGTTATTTGCCCGTTATGACCGCCTTCTCCTCCAGTCCTCCTTGGACCTGAT
  GGCAGATGTGGTGTACTGCCCCCGGCCGTGCTGCCAGCTGCCTGTGATGCAGGAACCTGGCTGCACCATG
  GGTATCTGCTCCAGCTGCAATTTTGCCTTCTGTACTTTGTGCAGGTTGACCTACCATGGGGTCTCCCCAT
  GTAAGGTGACTGCAGAGAAATTAATGGACTTACGAAATGAATACCTGCAAGCGGATGAGGCTAATAAAAG
  ACTTTTGGATCAAAGGTATGGTAAGAGAGTGATTCAGAAGGCACTGGAAGAGATGGAAAGTAAGGAGTGG
  CTAGAGAAGAACTCAAAGAGCTGCCCATGTTGTGGAACTCCCATAGAGAAATTAGACGGATGTAACAAGA
  TGACATGTACTGGCTGTATGCAATATTTCTGTTGGATTTGCATGGGTTCTCTCTCTAGAGCAAACCCTTA
  CAAACATTTCAATGACCCTGGTTCACCATGTTTTAACCGGCTGTTTTATGCTGTGGATGTTGACGACGAT
  ATTTGGGAAGATGAGGTAGAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223491 protein sequence
  Red=Cloning site Green=Tags(s)
  
  XCRQKIEKLRRMNCWPWQVFTMEMNLEKQSLSKVEKPGSIWICHRISRYL*AAIQMSVSRIVALNTPFAF
  CLHLC*TLNCHQIIHPLPHLHSHLVANGCHQLSYLLYAST*TTYGKNTVAAWSCLPGCNFLRKRP*HT*I
  LSLLLSSRLVLRKKCREGQLKLLPTQS*ILEELLDLM*TKRKLWMREQCRMWNHCQI*SRKSWTLIKLSR
  *NALIVNCSCAVSVSVRSWVVNACTSWSAGMCTAKPV*RTTLKSRSEMARFNASTAQNQSALRWPLLVRS
  KS*WKQSYLPVMTAFSSSPPWT*WQMWCTAPGRAASCL*CRNLAAPWVSAPAAILPSVLCAG*PTMGSPH
  VR*LQRN*WTYEMNTCKRMRLIKDFWIKGMVRE*FRRHWKRWKVRSG*RRTQRAAHVVELP*RN*TDVTR
  *HVLAVCNISVGFAWVLSLEQTLTNISMTLVHHVLTGCFMLWMLTTIFGKMR*K
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183399
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183399.1, NM_183399.2, NP_899646.1
• RefSeq Size: 4007 bp
• RefSeq ORF: 1425 bp
• Locus ID: 9604
• Protein Families: Druggable Genome, Transcription Factors
• MW: 53.8 kDa
• Gene Summary: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]