DCTN1 (NM_023019) Human Tagged ORF Clone

CAT#: RC223556

DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCTN1
• Synonyms: DAP-150; DP-150; P135
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223556 representing NM_023019
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGACAGGCACCGACAGCCCGAAAGACCACAACTCGGCGACCCAAGCCCACGCGCCCAGCCAGTA
  CTGGGGTGGCTGGGGCCAGTAGCTCCCTGGGCCCCTCTGGCTCAGCGTCAGCAGGTGAGCTGAGCAGCAG
  TGAGCCCAGCACCCCGGCTCAGACTCCGCTGGCAGCACCCATCATCCCCACGCCGGTCCTCACCTCTCCT
  GGAGCAGTCCCCCCGCTTCCTTCCCCATCCAAGGAGGAGGAGGGACTAAGGGCTCAGGTGCGGGACCTGG
  AGGAGAAACTAGAGACCCTGAGACTGAAACGGGCAGAAGACAAAGCAAAGCTAAAAGAGCTGGAGAAACA
  CAAAATCCAGCTGGAGCAGGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCCGACCTGCAGCGG
  CGCCTCAAGGAGGCGAGAAAGGAAGCCAAGGAGGCGCTGGAGGCAAAGGAACGCTATATGGAGGAGATGG
  CTGATACTGCTGATGCCATTGAGATGGCCACTTTGGACAAGGAGATGGCTGAAGAGCGGGCTGAGTCCCT
  GCAGCAGGAGGTGGAGGCACTGAAGGAGCGGGTGGACGAGCTCACTACTGACTTAGAGATCCTCAAGGCT
  GAGATTGAAGAGAAGGGCTCAGATGGCGCTGCATCCAGTTATCAGCTCAAGCAGCTTGAGGAGCAGAATG
  CCCGCCTGAAGGATGCCCTGGTGAGGATGCGGGATCTTTCTTCCTCAGAGAAGCAGGAGCATGTGAAGCT
  CCAGAAGCTCATGGAAAAGAAGAACCAAGAGCTGGAAGTTGTGAGGCAACAGCGGGAGCGTCTGCAGGAG
  GAGCTAAGCCAGGCAGAGAGCACCATTGATGAGCTCAAGGAGCAGGTGGATGCTGCTCTGGGTGCTGAGG
  AGATGGTGGAGATGCTGACAGATCGGAACCTGAATCTGGAAGAGAAAGTGCGCGAGTTGAGGGAGACTGT
  GGGAGACTTGGAAGCGATGAATGAGATGAACGATGAGCTGCAGGAGAATGCACGTGAGACAGAACTGGAG
  CTGCGGGAGCAGCTGGACATGGCAGGCGCGCGGGTTCGTGAGGCCCAGAAGCGTGTGGAGGCAGCCCAGG
  AGACGGTTGCAGACTACCAGCAGACCATCAAGAAGTACCGCCAGCTGACCGCCCATCTACAGGATGTGAA
  TCGGGAACTGACAAACCAGCAGGAAGCATCTGTGGAGAGGCAACAGCAGCCACCTCCAGAGACCTTTGAC
  TTCAAAATCAAGTTTGCTGAGACTAAGGCCCATGCCAAGGCAATTGAGATGGAATTGAGGCAGATGGAGG
  TGGCCCAGGCCAATCGACACATGTCCCTGCTGACAGCCTTCATGCCTGACAGCTTCCTTCGGCCAGGTGG
  GGACCATGACTGCGTTCTGGTGCTGTTGCTCATGCCTCGTCTCATTTGCAAGGCAGAGCTGATCCGGAAG
  CAGGCCCAGGAGAAGTTTGAACTAAGTGAGAACTGTTCAGAGCGGCCTGGGCTGCGAGGAGCTGCTGGGG
  AGCAACTCAGCTTTGCTGCTGGACTGGTGTACTCGCTGAGCCTGCTGCAGGCCACGCTACACCGCTATGA
  GCATGCCCTCTCTCAGTGCAGTGTGGATGTGTATAAGAAAGTGGGCAGCCTGTACCCTGAGATGAGTGCC
  CATGAGCGCTCCTTGGATTTCCTCATTGAACTGCTGCACAAGGATCAGCTGGATGAGACTGTCAATGTGG
  AGCCTCTCACCAAGGCCATCAAGTACTATCAGCATCTGTACAGCATCCACCTTGCCGAACAGCCTGAGGA
  CTGTACTATGCAGCTGGCTGACCACATTAAGTTCACGCAGAGTGCTCTGGACTGCATGAGTGTGGAGGTA
  GGACGGCTGCGTGCCTTCTTGCAGGGTGGGCAGGAGGCTACAGATATTGCCCTCCTGCTCCGGGATCTGG
  AAACTTCATGCAGTGACATCCGCCAGTTCTGCAAGAAGATCCGAAGGCGAATGCCAGGGACAGATGCTCC
  TGGGATCCCAGCTGCACTGGCCTTTGGACCACAGGTATCTGACACGCTCCTAGACTGCAGGAAACACTTG
  ACGTGGGTCGTGGCTGTGCTGCAGGAGGTGGCAGCTGCTGCTGCCCAGCTCATTGCCCCACTGGCAGAGA
  ATGAGGGGCTACTTGTGGCTGCTCTGGAGGAACTGGCTTTCAAAGCAAGCGAGCAGATCTATGGGACCCC
  CTCCAGCAGCCCCTATGAGTGTCTGCGCCAGTCATGCAACATCCTCATCAGTACCATGAACAAGCTGGCC
  ACAGCCATGCAGGAGGGGGAGTATGATGCAGAGCGGCCCCCCAGCAAGCCTCCACCGGTTGAACTGCGGG
  CTGCTGCCCTTCGTGCAGAGATCACAGATGCTGAAGGCCTGGGTTTGAAGCTCGAAGATCGAGAGACAGT
  TATTAAGGAGTTGAAGAAGTCACTCAAGATTAAGGGAGAGGAGCTAAGTGAGGCCAATGTGCGGCTGAGC
  CTCCTGGAGAAGAAGTTGGACAGTGCTGCCAAGGATGCAGATGAGCGCATCGAGAAAGTCCAGACTCGGC
  TGGAGGAGACCCAGGCACTGCTGCGAAAGAAGGAGAAAGAGTTTGAGGAGACAATGGATGCACTCCAGGC
  TGACATCGACCAGCTGGAGGCAGAGAAGGCAGAACTAAAGCAGCGTCTGAACAGCCAGTCCAAACGCACG
  ATTGAGGGACTCCGGGGCCCTCCTCCTTCAGGCATTGCTACTCTGGTCTCTGGCATTGCTGGTGAAGAAC
  AGCAGCGAGGAGCCATCCCTGGGCAGGCTCCAGGGTCTGTGCCAGGCCCAGGGCTGGTGAAGGACTCACC
  ACTGCTGCTTCAGCAGATCTCTGCCATGAGGCTGCACATCTCCCAGCTCCAGCATGAGAACAGCATCCTC
  AAGGGAGCCCAGATGAAGGCATCCTTGGCATCCCTGCCCCCTCTGCATGTTGCAAAGCTATCCCATGAGG
  GCCCTGGCAGTGAGTTACCAGCTGGAGCGCTGTATCGTAAGACCAGCCAGCTGCTGGAGACATTGAATCA
  ATTGAGCACACACACGCACGTAGTAGACATCACTCGCACCAGCCCTGCTGCCAAGAGCCCGTCGGCCCAA
  CTTATGGAGCAAGTGGCTCAGCTTAAGTCCCTGAGTGACACCGTCGAGAAGCTCAAGGATGAGGTCCTCA
  AGGAGACAGTATCTCAGCGCCCTGGAGCCACAGTACCCACTGACTTTGCCACCTTCCCTTCATCAGCCTT
  CCTCAGGGCCAAGGAGGAGCAGCAGGATGACACAGTCTACATGGGCAAAGTGACCTTCTCATGTGCGGCT
  GGTTTTGGACAGCGACACCGGCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGTCGCCTCATCT
  CC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223556 representing NM_023019
  Red=Cloning site Green=Tags(s)
  
  MMRQAPTARKTTTRRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSP
  GAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQR
  RLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKA
  EIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQE
  ELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELE
  LREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFD
  FKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRK
  QAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSA
  HERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEV
  GRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHL
  TWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLA
  TAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLS
  LLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRT
  IEGLRGPPPSGIATLVSGIAGEEQQRGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSIL
  KGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQ
  LMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAA
  GFGQRHRLVLTQEQLHQLHSRLIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023019
• ORF Size: 3432 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023019.1, NM_023019.2, NM_023019.3, NP_075408.1
• RefSeq Size: 4181 bp
• RefSeq ORF: 3435 bp
• Locus ID: 1639
• Cytogenetics: 2p13.1
• Domains: M
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• MW: 127.4 kDa
• Gene Summary: 'This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]'