EIF2B3 (NM_020365) Human Tagged ORF Clone

CAT#: RC223570

EIF2B3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa (EIF2B3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412535) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223570 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EIF2B3 protein (Cat# TP323570). The protein was produced from HEK293T cells transfected with EIF2B3 cDNA clone (Cat# RC223570) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF2B3
• Synonyms: EIF-2B; EIF2Bgamma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223570 representing NM_020365
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATTTCAAGCAGTAGTGATGGCAGTAGGTGGAGGATCTCGGATGACAGACCTAACTTCCAGCATTC
  CCAAACCTCTGCTTCCAGTTGGGAACAAACCTTTAATTTGGTACCCATTGAACCTGCTTGAGCGTGTTGG
  ATTTGAAGAAGTCATTGTGGTTACAACCAGGGATGTTCAAAAGGCTCTATGTGCAGAATTCAAGATGAAA
  ATGAAGCCAGATATTGTGTGTATTCCTGATGACGCTGACATGGGAACTGCAGATTCTTTGCGCTACATAT
  ATCCAAAACTTAAGACAGATGTGCTGGTGCTGAGCTGTGATCTGATAACAGACGTTGCCTTACATGAGGT
  TGTGGACCTGTTTAGAGCTTATGATGCATCACTTGCTATGTTGATGAGAAAAGGCCAAGATAGCATAGAA
  CCTGTTCCCGGTCAAAAGGGGAAAAAAAAAGCAGTGGAGCAGCGTGACTTCATTGGAGTGGACAGCACAG
  GAAAGAGGCTGCTCTTCATGGCTAATGAAGCAGACTTGGATGAAGAGCTGGTCATTAAGGGATCCATCCT
  ACAGAAGCATCCTAGAATACGTTTCCACACGGGTCTTGTGGATGCCCACCTCTACTGTTTGAAAAAATAC
  ATCGTGGATTTCCTAATGGAAAATGGGTCAATAACTTCTATCCGGAGTGAACTGATTCCATATTTAGTGA
  GAAAACAGTTTTCCTCAGCTTCCTCACAACAGGGACAAGAAGAAAAAGAGGAGGATCTAAAGAAAAAGGA
  GCTGAAGTCCTTAGATATCTACAGTTTTATAAAAGAAGCCAATACACTGAACCTGGCTCCCTATGATGCC
  TGCTGGAATGCCTGTCGAGGAGACAGGTGGGAAGACTTGTCCAGATCACAGGTGCGCTGCTATGTCCACA
  TCATGAAAGAGGGGCTCTGCTCTCGAGTGAGCACACTGGGACTCTACATGGAAGCAAACAGACAGGTGCC
  CAAATTGCTGTCTGCTCTCTGTCCAGAAGAACCACCAGTCCATTCGTCAGCCCAGATTGTCAGCAAACAC
  CTGGTTGGAGTTGACAGCCTCATTGGGCCAGAGACACAGATTGGAGAGAAGTCATCCATTAAGCGCTCAG
  TCATTGGCTCATCCTGTCTCATAAAAGATAGAGTGACTATTACCAATTGCCTTCTCATGAACTCAGTCAC
  TGTGGAGGAAGGAAGCAATATCCAAGGCAGTGTCATCTGCAACAATGCTGTGATCGAGAAGGGTGCAGAC
  ATCAAGGACTGCTTGATTGGAAGTGGCCAGAGGATTGAAGCCAAAGCTAAACGAGTGAATGAGGTGATCG
  TGGGGAATGACCAGCTCATGGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223570 representing NM_020365
  Red=Cloning site Green=Tags(s)
  
  MEFQAVVMAVGGGSRMTDLTSSIPKPLLPVGNKPLIWYPLNLLERVGFEEVIVVTTRDVQKALCAEFKMK
  MKPDIVCIPDDADMGTADSLRYIYPKLKTDVLVLSCDLITDVALHEVVDLFRAYDASLAMLMRKGQDSIE
  PVPGQKGKKKAVEQRDFIGVDSTGKRLLFMANEADLDEELVIKGSILQKHPRIRFHTGLVDAHLYCLKKY
  IVDFLMENGSITSIRSELIPYLVRKQFSSASSQQGQEEKEEDLKKKELKSLDIYSFIKEANTLNLAPYDA
  CWNACRGDRWEDLSRSQVRCYVHIMKEGLCSRVSTLGLYMEANRQVPKLLSALCPEEPPVHSSAQIVSKH
  LVGVDSLIGPETQIGEKSSIKRSVIGSSCLIKDRVTITNCLLMNSVTVEEGSNIQGSVICNNAVIEKGAD
  IKDCLIGSGQRIEAKAKRVNEVIVGNDQLMEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020365
• ORF Size: 1356 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020365.1, NM_020365.2, NM_020365.3, NM_020365.4, NP_065098.1
• RefSeq Size: 1602
• RefSeq ORF: 1359
• Locus ID: 8891
• Domains: NTP_transferase
• MW: 50.1 kDa
• Gene Summary: The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]