HS2ST1 (NM_012262) Human Tagged ORF Clone

CAT#: RC223657

HS2ST1 (Myc-DDK-tagged)-Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402186) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223657 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HS2ST1
• Synonyms: dJ604K5.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223657 representing NM_012262
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTCCTCAGGATTATGATGCCGCCCAAGTTGCAGCTGCTGGCGGTGGTGGCCTTCGCGGTGGCGA
  TGCTCTTCTTGGAAAACCAGATCCAGAAACTGGAGGAGTCCCGCTCGAAGCTAGAAAGGGCTATTGCAAG
  ACACGAAGTCCGAGAAATTGAGCAGCGACATACAATGGATGGCCCTCGGCAAGATGCCACTTTAGATGAG
  GAAGAGGACATGGTGATCATTTATAACAGAGTTCCCAAAACGGCAAGCACTTCATTTACCAATATCGCCT
  ATGACCTGTGTGCAAAGAATAAATACCATGTCCTTCATATCAACACTACCAAAAATAATCCAGTGATGTC
  ATTGCAAGATCAGGTGCGCTTTGTAAAGAATATAACTTCCTGGAAAGAGATGAAACCAGGATTTTATCAT
  GGACACGTTTCTTACTTGGATTTTGCAAAATTTGGTGTGAAGAAGAAACCAATTTACATTAATGTCATAA
  GGGATCCTATTGAGAGGCTAGTTTCTTATTATTACTTTCTGAGATTTGGAGATGATTATAGACCAGGGTT
  ACGGAGACGAAAACAAGGAGACAAAAAGACCTTTGATGAATGTGTAGCAGAAGGTGGCTCAGACTGTGCT
  CCAGAGAAGCTCTGGCTTCAAATCCCGTTCTTCTGTGGCCATAGCTCCGAATGCTGGAATGTGGGAAGCA
  GGTGGGCTATGGATCAAGCCAAGTATAACCTAATTAATGAATATTTTCTGGTGGGAGTTACTGAAGAACT
  TGAAGATTTTATCATGTTATTGGAGGCAGCATTGCCCCGGTTTTTCAGGGGTGCTACTGAACTCTATCGC
  ACAGGAAAGAAATCTCATCTTAGGAAAACCACAGAGAAGAAACTCCCCACTAAACAAACCATTGCAAAAC
  TACAGCAATCTGATATTTGGAAAATGGAGAATGAGTTCTATGAATTTGCACTAGAGCAGTTCCAATTCAT
  CAGAGCCCATGCCGTTCGAGAAAAAGATGGAGACCTCTACATCCTCGCACAAAACTTTTTCTATGAAAAG
  ATTTACCCTAAGTCGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223657 representing NM_012262
  Red=Cloning site Green=Tags(s)
  
  MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRSKLERAIARHEVREIEQRHTMDGPRQDATLDE
  EEDMVIIYNRVPKTASTSFTNIAYDLCAKNKYHVLHINTTKNNPVMSLQDQVRFVKNITSWKEMKPGFYH
  GHVSYLDFAKFGVKKKPIYINVIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAEGGSDCA
  PEKLWLQIPFFCGHSSECWNVGSRWAMDQAKYNLINEYFLVGVTEELEDFIMLLEAALPRFFRGATELYR
  TGKKSHLRKTTEKKLPTKQTIAKLQQSDIWKMENEFYEFALEQFQFIRAHAVREKDGDLYILAQNFFYEK
  IYPKSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012262
• ORF Size: 1068 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012262.1, NM_012262.2, NM_012262.3, NP_036394.1
• RefSeq Size: 6708 bp
• RefSeq ORF: 1071 bp
• Locus ID: 9653
• Domains: HS2ST
• Protein Pathways: Heparan sulfate biosynthesis
• MW: 41.7 kDa
• Gene Summary: Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Aug 2008]