GLYAT (NM_201648) Human Tagged ORF Clone

CAT#: RC223667

GLYAT (Myc-DDK-tagged)-Human glycine-N-acyltransferase (GLYAT), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY404498) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223667 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GLYAT
• Synonyms: ACGNAT; CAT; GAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223667 representing NM_201648
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTTACCATTGCAAGGTGCCCAGATGCTGCAGATGCTGGAGAAATCCTTGAGGAAGAGCCTCCCAG
  CATCCTTAAAGGTTTATGGAACTGTCTTTCACATAAACCATGGAAATCCATTCAATCTGAAGGCTGTGGT
  GGACAAGTGGCCTGATTTTAATACAGTGGTTGTCTGCCCTCAGGAGCAGGATATGACAGATGACCTTGAT
  CACTATACCAATACTTACCAAATCTACTCCAAAGATCCCCAAAACTGTCAGGAATTCCTTGGATCACCAG
  AACTCATCAACTGGAAACAGCATTTACAGATTCAAAGTTCACAGCCTAGCCTGAATGAGGCTATACAAAA
  TCTTGCAGCCATTAAGTCCTTCAAAGTCAAACAAACACAACGCATTCTCTATATGGCAGCTGAAACAGCC
  AAGGAACTGACTCCTTTCCTGCTGAAATCAAAGATTTTATCTCCCAATGGTGGCAAACCCAAGGCCATCA
  ACCAAGAGATGTTTAAACTCTCATCCATGGATGTTACCCATGCTCACTTGGTGAATAAATTCTGGCATTT
  TGGTGGTAATGAGAGGAGCCAGAGATTCATTGAGCGCTGCATTCAGACCTTTCCCACCTGCTGTCTCCTG
  GGGCCTGAGGGGACCCCTGTGTGCTGGGATCTAATGGACCAGACTGGAGAGATGAGAATGGCAGGCACCT
  TGCCGGAATACCGGCTCCATGGCCTTGTGACGTATGTCATCTATTCCCACGCCCAGAAATTGGGCAAACT
  TGGGTTTCCTGTCTATTCTCATGTAGACTACAGCAATGAAGCTATGCAAAAAATGAGTTACACACTGCAA
  CATGTTCCCATTCCCAGAAGCTGGAACCAGTGGAACTGTGTACCTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223667 representing NM_201648
  Red=Cloning site Green=Tags(s)
  
  MMLPLQGAQMLQMLEKSLRKSLPASLKVYGTVFHINHGNPFNLKAVVDKWPDFNTVVVCPQEQDMTDDLD
  HYTNTYQIYSKDPQNCQEFLGSPELINWKQHLQIQSSQPSLNEAIQNLAAIKSFKVKQTQRILYMAAETA
  KELTPFLLKSKILSPNGGKPKAINQEMFKLSSMDVTHAHLVNKFWHFGGNERSQRFIERCIQTFPTCCLL
  GPEGTPVCWDLMDQTGEMRMAGTLPEYRLHGLVTYVIYSHAQKLGKLGFPVYSHVDYSNEAMQKMSYTLQ
  HVPIPRSWNQWNCVPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201648
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_201648.1, NM_201648.2, NP_964011.1, NP_964011.2
• RefSeq Size: 2068
• RefSeq ORF: 891
• Locus ID: 10249
• MW: 33.7 kDa
• Gene Summary: The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]