PLEKHO2 (NM_025201) Human Tagged ORF Clone

CAT#: RC223676

PLEKHO2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family O member 2 (PLEKHO2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410849) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223676 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PLEKHO2 protein (Cat# TP323676). The protein was produced from HEK293T cells transfected with PLEKHO2 cDNA clone (Cat# RC223676) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEKHO2
• Synonyms: PLEKHQ1; PP1628; pp9099
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223676 representing NM_025201
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGAGGGTGTGAAGGAAGCCGGTGAGAAGCCTCGGGGAGCACAGATGGTGGACAAGGCTGGCT
  GGATCAAGAAGAGCAGTGGGGGCCTCCTGGGTTTCTGGAAAGACCGATATCTGCTCCTCTGCCAGGCCCA
  GCTGCTGGTCTATGAGAATGAGGATGATCAGAAGTGTGTGGAGACTGTGGAGCTGGGCAGCTATGAGAAG
  TGCCAGGACCTTCGTGCCCTCCTCAAGCGAAAACACCGCTTTATCCTGCTGCGATCCCCAGGGAACAAGG
  TCAGCGACATCAAATTCCAGGCACCCACCGGGGAGGAGAAGGAATCCTGGATCAAAGCCCTCAATGAAGG
  GATTAACCGAGGCAAAAACAAGGCTTTCGATGAGGTAAAGGTGGACAAGAGCTGCGCCCTGGAGCATGTG
  ACACGGGACCGGGTGCGAGGGGGCCAGCGACGCCGGCCACCAACGAGAGTCCACCTGAAGGAGGTGGCCA
  GTGCAGCTTCTGACGGTCTTCTGCGCCTGGATCTTGATGTTCCGGACAGTGGGCCACCAGTGTTTGCCCC
  CAGCAATCATGTCAGTGAAGCCCAACCTCGGGAGACACCCCGGCCCCTCATGCCTCCTACCAAGCCTTTC
  CTAGCACCTGAGACCACCAGCCCTGGTGACAGGGTGGAGACCCCTGTGGGGGAGAGAGCCCCAACCCCTG
  TCTCAGCAAGCTCTGAGGTCTCCCCTGAGAGCCAAGAGGACTCAGAGACCCCAGCAGAGGAGGACAGTGG
  CTCTGAGCAGCCTCCCAACAGCGTCCTGCCTGACAAACTGAAGGTGAGCTGGGAGAACCCCAGCCCCCAG
  GAGGCCCCTGCTGCAGAGAGTGCAGAACCGTCCCAGGCACCCTGTTCTGAGACTTCTGAGGCTGCCCCCA
  GGGAGGGTGGGAAGCCCCCTACACCCCCACCCAAGATCTTATCAGAGAAACTGAAAGCCTCCATGGGTGA
  GATGCAGGCTTCTGGGCCACCTGCTCCAGGCACAGTGCAGGTCTCAGTGAATGGCATGGATGACAGTCCT
  GAGCCTGCCAAGCCCTCTCAGGCTGAGGGCACCCCAGGAACTCCTCCAAAGGATGCAACAACATCCACAG
  CACTGCCCCCCTGGGACCTGCCACCTCAGTTCCATCCCCGCTGCTCCTCCCTTGGGGACTTGCTTGGGGA
  AGGCCCGCGGCATCCCTTGCAGCCCAGGGAACGGCTATATCGGGCCCAGCTGGAGGTGAAGGTGGCCTCG
  GAACAGACGGAGAAACTGTTGAACAAGGTGCTGGGCAGTGAGCCGGCCCCTGTTAGTGCCGAAACATTGC
  TCAGCCAGGCTGTGGAGCAGCTGAGGCAGGCCACCCAGGTCCTGCAGGAAATGAGAGATTTGGGAGAGCT
  GAGCCAGGAAGCACCTGGGCTAAGGGAGAAGCGGAAGGAGCTGGTGACCCTCTACAGGAGAAGTGCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223676 representing NM_025201
  Red=Cloning site Green=Tags(s)
  
  MEEEGVKEAGEKPRGAQMVDKAGWIKKSSGGLLGFWKDRYLLLCQAQLLVYENEDDQKCVETVELGSYEK
  CQDLRALLKRKHRFILLRSPGNKVSDIKFQAPTGEEKESWIKALNEGINRGKNKAFDEVKVDKSCALEHV
  TRDRVRGGQRRRPPTRVHLKEVASAASDGLLRLDLDVPDSGPPVFAPSNHVSEAQPRETPRPLMPPTKPF
  LAPETTSPGDRVETPVGERAPTPVSASSEVSPESQEDSETPAEEDSGSEQPPNSVLPDKLKVSWENPSPQ
  EAPAAESAEPSQAPCSETSEAAPREGGKPPTPPPKILSEKLKASMGEMQASGPPAPGTVQVSVNGMDDSP
  EPAKPSQAEGTPGTPPKDATTSTALPPWDLPPQFHPRCSSLGDLLGEGPRHPLQPRERLYRAQLEVKVAS
  EQTEKLLNKVLGSEPAPVSAETLLSQAVEQLRQATQVLQEMRDLGELSQEAPGLREKRKELVTLYRRSAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025201
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025201.3, NM_025201.4, NP_079477.2
• RefSeq Size: 3675
• RefSeq ORF: 1473
• Locus ID: 80301
• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction
• MW: 53.2 kDa