LARGE (LARGE1) (NM_133642) Human Tagged ORF Clone

CAT#: RC223788

LARGE (Myc-DDK-tagged)-Human like-glycosyltransferase (LARGE), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY408762) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223788 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LARGE1
• Synonyms: LARGE; MDC1D; MDDGA6; MDDGB6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223788 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGAATCTGCAGGGGGAGACGGAAATTCTTGGCTGCCTCGTTGAGTCTTCTCTGCATCCCAGCCA
  TCACCTGGATTTACCTGTTTTCTGGGAGCTTCGAAGATGGAAAGCCCGTGTCTCTGTCACCGCTGGAGTC
  CCAGGCACACAGCCCCAGGTACACGGCCTCCAGCCAGCGGGAGCGCGAGAGCCTGGAGGTGCGCATGCGC
  GAGGTGGAGGAGGAGAACCGCGCCCTCCGCAGGCAGCTCAGCCTGGCCCAGGGCCGAGCCCCATCCCATC
  GCCGAGGCAACCACTCCAAGACCTACTCCATGGAGGAGGGCACTGGAGACAGCGAGAACCTTCGGGCTGG
  CATCGTGGCAGGCAACAGCTCCGAGTGTGGGCAGCAGCCGGTCGTGGAGAAATGCGAGACAATCCACGTT
  GCTATTGTCTGCGCCGGATACAATGCCAGCCGGGATGTCGTCACCCTGGTCAAATCCGTCCTGTTCCATA
  GACGGAACCCTCTGCACTTCCACCTTATTGCTGACTCCATTGCGGAGCAGATCCTGGCCACGCTCTTCCA
  GACCTGGATGGTGCCCGCTGTGCGTGTGGACTTCTACAATGCAGACGAGCTCAAGTCTGAAGTTTCCTGG
  ATCCCCAATAAACATTACTCTGGGATTTATGGTCTGATGAAGCTTGTCCTGACCAAGACTCTTCCTGCCA
  ACCTGGAGAGAGTCATCGTCCTTGACACGGATATCACCTTTGCCACTGACATTGCAGAGCTGTGGGCTGT
  GTTCCACAAGTTCAAAGGTCAGCAAGTCCTGGGCTTGGTGGAGAACCAGAGTGACTGGTACCTTGGAAAC
  CTGTGGAAAAATCACCGCCCATGGCCAGCCCTTGGAAGAGGCTACAACACAGGGGTGATCCTGTTACTTC
  TGGATAAGCTGCGGAAGATGAAATGGGAGCAGATGTGGAGGCTGACCGCAGAGAGGGAGCTCATGGGCAT
  GCTCTCTACATCCTTAGCTGACCAGGATATTTTCAATGCCGTCATCAAACAAAACCCCTTCCTTGTGTAC
  CAGCTCCCCTGCTTCTGGAATGTGCAGCTGTCAGACCACACCCGCTCCGAGCAGTGCTACAGAGACGTGT
  CTGATCTAAAGGTCATTCACTGGAACTCCCCCAAGAAGCTCCGGGTGAAGAACAAGCATGTGGAGTTTTT
  TCGCAACCTCTACCTGACCTTCCTGGAGTATGACGGCAATCTTCTGAGGCGGGAACTGTTTGGCTGCCCC
  AGTGAGGCTGATGTCAACAGTGAAAACCTCCAGAAGCAGCTGTCTGAGCTGGACGAGGACGACCTGTGCT
  ATGAGTTCCGGCGAGAGCGCTTCACTGTCCACCGCACCCACCTGTACTTCCTGCACTACGAGTATGAGCC
  TGCAGCAGACAGCACGGACGTCACCCTGGTCGCTCAGCTGTCCATGGACAGGCTCCAGATGCTGGAGGCC
  ATCTGCAAGCACTGGGAGGGGCCCATCAGCCTGGCCCTCTACCTGTCAGACGCCGAGGCCCAGCAGTTCC
  TCCGCTACGCACAGGGCTCTGAGGTGCTTATGAGCCGCCACAACGTGGGCTACCACATCGTGTACAAGGA
  GGGCCAGTTCTACCCCGTGAACCTGCTGCGCAACGTGGCCATGAAGCACATCAGCACTCCCTACATGTTC
  CTGTCTGACATTGACTTCCTGCCCATGTATGGGCTCTATGAGTACCTCAGGAAGTCTGTCATCCAGCTCG
  ATCTTGCCAACACCAAGAAAGCAATGATTGTCCCCGCGTTCGAGACACTGCGCTACCGGCTGTCCTTCCC
  CAAGTCAAAAGCGGAGTTGCTGTCAATGCTGGACATGGGGACCCTCTTCACATTCAGGTACCACGTCTGG
  ACGAAAGGCCACGCACCCACAAACTTCGCCAAGTGGCGGACCGCCACCACGCCTTACCGGGTTGAGTGGG
  AGGCCGATTTTGAGCCGTATGTTGTTGTGAGACGTGACTGCCCGGAGTACGACCGGAGGTTTGTAGGCTT
  TGGCTGGAACAAAGTGGCTCATATCATGGAGCTGGATGTGCAGGAGTATGAGTTCATTGTGCTGCCCAAC
  GCCTACATGATCCACATGCCTCATGCCCCCAGCTTCGACATTACCAAGTTCCGTTCCAACAAGCAATACC
  GCATCTGTCTCAAAACCCTCAAGGAAGAGTTTCAGCAGGACATGTCCCGCCGCTACGGCTTTGCTGCCCT
  GAAATATCTCACAGCCGAGAACAACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223788 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLGICRGRRKFLAASLSLLCIPAITWIYLFSGSFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRMR
  EVEEENRALRRQLSLAQGRAPSHRRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPVVEKCETIHV
  AIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSW
  IPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLVENQSDWYLGN
  LWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLSTSLADQDIFNAVIKQNPFLVY
  QLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRRELFGCP
  SEADVNSENLQKQLSELDEDDLCYEFRRERFTVHRTHLYFLHYEYEPAADSTDVTLVAQLSMDRLQMLEA
  ICKHWEGPISLALYLSDAEAQQFLRYAQGSEVLMSRHNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMF
  LSDIDFLPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRYHVW
  TKGHAPTNFAKWRTATTPYRVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKVAHIMELDVQEYEFIVLPN
  AYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSRRYGFAALKYLTAENNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133642
• ORF Size: 2268 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133642.1, NM_133642.2, NM_133642.3, NP_598397.1
• RefSeq Size: 4138 bp
• RefSeq ORF: 2271 bp
• Locus ID: 9215
• Domains: Glyco_transf_8
• Protein Families: Druggable Genome, Transmembrane
• MW: 88.1 kDa
• Gene Summary: This gene encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. The protein encoded by this gene is the glycotransferase that adds the final xylose and glucuronic acid to alpha-dystroglycan and thereby allows alpha-dystroglycan to bind ligands including laminin 211 and neurexin. Mutations in this gene cause several forms of congenital muscular dystrophy characterized by cognitive disability and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2018]