TCP1 eta (CCT7) (NM_001009570) Human Tagged ORF Clone
CAT#: RC223866
CCT7 (Myc-DDK-tagged)-Human chaperonin containing TCP1, subunit 7 (eta) (CCT7), transcript variant 2
"NM_001009570" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CCT7 |
Synonyms | CCTETA; CCTH; NIP7-1; TCP1ETA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223866 representing NM_001009570
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGATTCTCAGCTGGTAGCTGGTGTTGCATTCAAGAAGACTTTCTCTTACGCTGGGTTTGAAATGC AACCCAAAAAGTACCACAATCCCAAGATTGCCCTTTTGAATGTCGAGCTCGAGTTGAAAGCTGAGAAAGA CAATGCTGAGATAAGAGTCCACACAGTTGAGGATTATCAGGCAATTGTTGATGCTGAGTGGAACATTCTC TATGACAAGTTAGAGAAGATCCATCATTCTGGAGCCAAAGTTGTCTTGTCCAAACTCCCCATTGGGGATG TGGCCACCCAGTACTTTGCTGACAGGGACATGTTCTGTGCTGGCCGAGTACCTGAGGAGGATCTGAAGAG GACAATGATGGCCTGTGGAGGCTCAATCCAGACCAGTGTGAATGCTCTGTCAGCAGATGTGCTGGGTCGA TGCCAGGTGTTTGAAGAGACCCAGATTGGAGGCGAGAGGTACAATTTTTTTACTGGCTGCCCCAAGGCCA AGACATGCACCTTCATTCTCCGTGGCGGCGCCGAGCAGTTTATGGAGGAGACAGAGCGGTCCCTGCATGA TGCCATCATGATCGTCAGGAGGGCCATCAAGAATGATTCAGTGGTGGCTGGTGGCGGGGCCATTGAGATG GAACTCTCCAAGTACCTGCGGGATTACTCAAGGACTATTCCAGGAAAACAGCAGCTGTTGATTGGGGCAT ATGCCAAGGCCTTGGAGATTATCCCACGCCAGCTGTGTGACAATGCTGGCTTTGATGCCACAAACATTCT CAACAAGCTGCGGGCTCGGCATGCCCAGGGGGGTACATGGTATGGAGTAGACATCAACAACGAGGACATT GCTGACAACTTTGAAGCTTTCGTGTGGGAGCCAGCTATGGTGCGGATCAATGCGCTGACAGCAGCCTCTG AGGCTGCGTGCCTGATCGTGTCTGTAGATGAAACCATCAAGAACCCCCGCTCGACTGTGGATGCTCCCAC AGCAGCAGGCCGGGGCCGTGGTCGTGGCCGCCCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223866 representing NM_001009570
Red=Cloning site Green=Tags(s) MMDSQLVAGVAFKKTFSYAGFEMQPKKYHNPKIALLNVELELKAEKDNAEIRVHTVEDYQAIVDAEWNIL YDKLEKIHHSGAKVVLSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGR CQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETERSLHDAIMIVRRAIKNDSVVAGGGAIEM ELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNAGFDATNILNKLRARHAQGGTWYGVDINNEDI ADNFEAFVWEPAMVRINALTAASEAACLIVSVDETIKNPRSTVDAPTAAGRGRGRGRPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001009570 |
ORF Size | 1017 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001009570.1, NM_001009570.2, NP_001009570.1 |
RefSeq Size | 1280 bp |
RefSeq ORF | 1020 bp |
Locus ID | 10574 |
Cytogenetics | 2p13.2 |
MW | 37.3 kDa |
Gene Summary | This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6. [provided by RefSeq, Oct 2009] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC301409 | CCT7 (untagged)-Human chaperonin containing TCP1, subunit 7 (eta) (CCT7), transcript variant 2 |
USD 580.00 |
|
RG223866 | CCT7 (GFP-tagged) - Human chaperonin containing TCP1, subunit 7 (eta) (CCT7), transcript variant 2 |
USD 460.00 |
|
RC223866L3 | Lenti ORF clone of Human chaperonin containing TCP1, subunit 7 (eta) (CCT7), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC223866L4 | Lenti ORF clone of Human chaperonin containing TCP1, subunit 7 (eta) (CCT7), transcript variant 2, mGFP tagged |
USD 620.00 |
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