HTR1B (NM_000863) Human Tagged ORF Clone
CAT#: RC223874
HTR1B (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B)
"NM_000863" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HTR1B |
Synonyms | 5-HT-1B; 5-HT-1D-beta; 5-HT1B; 5-HT1DB; HTR1D2; HTR1DB; S12 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223874 representing NM_000863
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAACCGGGTGCTCAGCGCGCTCCACCGCCGCCCGCGGGCTCCGAGACCTGGGTTCCTCAAGCCA ACTTATCCTCTGCTCCCTCCCAAAACTGCAGCGCCAAGGACTACATTTACCAGGACTCCATCTCCCTACC CTGGAAAGTACTGCTGGTTATGCTATTGGCGCTCATCACCTTGGCCACCACGCTCTCCAATGCCTTTGTG ATTGCCACAGTGTACCGGACCCGGAAACTGCACACCCCGGCTAACTACCTGATCGCCTCTCTGGCGGTCA CCGACCTGCTTGTGTCCATCCTGGTGATGCCCATCAGCACCATGTACACTGTCACCGGCCGCTGGACACT GGGCCAGGTGGTCTGTGACTTCTGGCTGTCGTCGGACATCACTTGTTGCACTGCCTCCATCCTGCACCTC TGTGTCATCGCCCTGGACCGCTACTGGGCCATCACGGACGCCGTGGAGTACTCAGCTAAAAGGACTCCCA AGAGGGCGGCGGTCATGATCGCGCTGGTGTGGGTCTTCTCCATCTCTATCTCGCTGCCGCCCTTCTTCTG GCGTCAGGCTAAGGCCGAAGAGGAGGTGTCGGAATGCGTGGTGAACACCGACCACATCCTCTACACGGTC TACTCCACGGTGGGTGCTTTCTACTTCCCCACCCTGCTCCTCATCGCCCTCTATGGCCGCATCTACGTAG AAGCCCGCTCCCGGATTTTGAAACAGACGCCCAACAGGACCGGCAAGCGCTTGACCCGAGCCCAGCTGAT AACCGACTCCCCCGGGTCCACGTCCTCGGTCACCTCTATTAACTCGCGGGTTCCCGACGTGCCCAGCGAA TCCGGATCTCCTGTGTATGTGAACCAAGTCAAAGTGCGAGTCTCCGACGCCCTGCTGGAAAAGAAGAAAC TCATGGCCGCTAGGGAGCGCAAAGCCACCAAGACCCTAGGGATCATTTTGGGAGCCTTTATTGTGTGTTG GCTACCCTTCTTCATCATCTCCCTAGTGATGCCTATCTGCAAAGATGCCTGCTGGTTCCACCTAGCCATC TTTGACTTCTTCACATGGCTGGGCTATCTCAACTCCCTCATCAACCCCATAATCTATACCATGTCCAATG AGGACTTTAAACAAGCATTCCATAAACTGATACGTTTTAAGTGCACAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223874 representing NM_000863
Red=Cloning site Green=Tags(s) MEEPGAQRAPPPPAGSETWVPQANLSSAPSQNCSAKDYIYQDSISLPWKVLLVMLLALITLATTLSNAFV IATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQVVCDFWLSSDITCCTASILHL CVIALDRYWAITDAVEYSAKRTPKRAAVMIALVWVFSISISLPPFFWRQAKAEEEVSECVVNTDHILYTV YSTVGAFYFPTLLLIALYGRIYVEARSRILKQTPNRTGKRLTRAQLITDSPGSTSSVTSINSRVPDVPSE SGSPVYVNQVKVRVSDALLEKKKLMAARERKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHLAI FDFFTWLGYLNSLINPIIYTMSNEDFKQAFHKLIRFKCTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000863 |
ORF Size | 1170 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000863.1, NM_000863.2, NP_000854.1 |
RefSeq Size | 1173 bp |
RefSeq ORF | 1173 bp |
Locus ID | 3351 |
Cytogenetics | 6q14.1 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
MW | 43.4 kDa |
Gene Summary | 'The protein encoded by this intronless gene is a G-protein coupled receptor for serotonin (5-hydroxytryptamine). Ligand binding activates second messengers that inhibit the activity of adenylate cyclase and manage the release of serotonin, dopamine, and acetylcholine in the brain. The encoded protein may be involved in several neuropsychiatric disorders and therefore is often a target of antidepressant and other psychotherapeutic drugs. [provided by RefSeq, Nov 2015]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119612 | HTR1B (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B) |
USD 310.00 |
|
RG223874 | HTR1B (GFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B) |
USD 460.00 |
|
RC223874L1 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B), Myc-DDK-tagged |
USD 768.00 |
|
RC223874L2 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B), mGFP tagged |
USD 620.00 |
|
RC223874L3 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B), Myc-DDK-tagged |
USD 620.00 |
|
RC223874L4 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B), mGFP tagged |
USD 620.00 |
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