HTR1B (NM_000863) Human Tagged ORF Clone

CAT#: RC223874

HTR1B (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B)

Product Images

Western blot validation of overexpression lysate (Cat# LY424477) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223874 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HTR1B
• Synonyms: 5-HT-1B; 5-HT-1D-beta; 5-HT1B; 5-HT1DB; HTR1D2; HTR1DB; S12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223874 representing NM_000863
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAACCGGGTGCTCAGCGCGCTCCACCGCCGCCCGCGGGCTCCGAGACCTGGGTTCCTCAAGCCA
  ACTTATCCTCTGCTCCCTCCCAAAACTGCAGCGCCAAGGACTACATTTACCAGGACTCCATCTCCCTACC
  CTGGAAAGTACTGCTGGTTATGCTATTGGCGCTCATCACCTTGGCCACCACGCTCTCCAATGCCTTTGTG
  ATTGCCACAGTGTACCGGACCCGGAAACTGCACACCCCGGCTAACTACCTGATCGCCTCTCTGGCGGTCA
  CCGACCTGCTTGTGTCCATCCTGGTGATGCCCATCAGCACCATGTACACTGTCACCGGCCGCTGGACACT
  GGGCCAGGTGGTCTGTGACTTCTGGCTGTCGTCGGACATCACTTGTTGCACTGCCTCCATCCTGCACCTC
  TGTGTCATCGCCCTGGACCGCTACTGGGCCATCACGGACGCCGTGGAGTACTCAGCTAAAAGGACTCCCA
  AGAGGGCGGCGGTCATGATCGCGCTGGTGTGGGTCTTCTCCATCTCTATCTCGCTGCCGCCCTTCTTCTG
  GCGTCAGGCTAAGGCCGAAGAGGAGGTGTCGGAATGCGTGGTGAACACCGACCACATCCTCTACACGGTC
  TACTCCACGGTGGGTGCTTTCTACTTCCCCACCCTGCTCCTCATCGCCCTCTATGGCCGCATCTACGTAG
  AAGCCCGCTCCCGGATTTTGAAACAGACGCCCAACAGGACCGGCAAGCGCTTGACCCGAGCCCAGCTGAT
  AACCGACTCCCCCGGGTCCACGTCCTCGGTCACCTCTATTAACTCGCGGGTTCCCGACGTGCCCAGCGAA
  TCCGGATCTCCTGTGTATGTGAACCAAGTCAAAGTGCGAGTCTCCGACGCCCTGCTGGAAAAGAAGAAAC
  TCATGGCCGCTAGGGAGCGCAAAGCCACCAAGACCCTAGGGATCATTTTGGGAGCCTTTATTGTGTGTTG
  GCTACCCTTCTTCATCATCTCCCTAGTGATGCCTATCTGCAAAGATGCCTGCTGGTTCCACCTAGCCATC
  TTTGACTTCTTCACATGGCTGGGCTATCTCAACTCCCTCATCAACCCCATAATCTATACCATGTCCAATG
  AGGACTTTAAACAAGCATTCCATAAACTGATACGTTTTAAGTGCACAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223874 representing NM_000863
  Red=Cloning site Green=Tags(s)
  
  MEEPGAQRAPPPPAGSETWVPQANLSSAPSQNCSAKDYIYQDSISLPWKVLLVMLLALITLATTLSNAFV
  IATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQVVCDFWLSSDITCCTASILHL
  CVIALDRYWAITDAVEYSAKRTPKRAAVMIALVWVFSISISLPPFFWRQAKAEEEVSECVVNTDHILYTV
  YSTVGAFYFPTLLLIALYGRIYVEARSRILKQTPNRTGKRLTRAQLITDSPGSTSSVTSINSRVPDVPSE
  SGSPVYVNQVKVRVSDALLEKKKLMAARERKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHLAI
  FDFFTWLGYLNSLINPIIYTMSNEDFKQAFHKLIRFKCTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000863
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000863.1, NM_000863.2, NP_000854.1
• RefSeq Size: 1173 bp
• RefSeq ORF: 1173 bp
• Locus ID: 3351
• Cytogenetics: 6q14.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 43.4 kDa
• Gene Summary: 'The protein encoded by this intronless gene is a G-protein coupled receptor for serotonin (5-hydroxytryptamine). Ligand binding activates second messengers that inhibit the activity of adenylate cyclase and manage the release of serotonin, dopamine, and acetylcholine in the brain. The encoded protein may be involved in several neuropsychiatric disorders and therefore is often a target of antidepressant and other psychotherapeutic drugs. [provided by RefSeq, Nov 2015]'