RDH8 (NM_015725) Human Tagged ORF Clone

CAT#: RC223945

RDH8 (Myc-DDK-tagged)-Human retinol dehydrogenase 8 (all-trans) (RDH8)

Product Images

Western blot validation of overexpression lysate (Cat# LY414359) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223945 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RDH8
• Synonyms: PRRDH; SDR28C2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223945 representing NM_015725
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCTGCACCCCGGACTGTGTTGATCTCCGGCTGCTCATCAGGAATTGGTCTGGAACTTGCAGTGC
  AACTGGCCCATGACCCCAAGAAGCGCTACCAGGTCGTGGCCACCATGAGGGACCTGGGGAAGAAGGAGAC
  ACTGGAGGCAGCTGCTGGGGAGGCTCTGGGGCAGACCCTCACCGTGGCCCAGCTGGACGTGTGCAGTGAT
  GAGTCGGTGGCCCAGTGTCTCAGCTGTATCCAGGGAGAAGTGGACGTGCTGGTGAATAATGCTGGAATGG
  GCCTGGTGGGGCCCCTGGAGGGGCTCAGCCTTGCTGCCATGCAGAATGTCTTTGACACCAACTTTTTCGG
  AGCTGTCCGTCTCGTCAAAGCTGTGCTTCCAGGCATGAAGAGGAGGCGGCAGGGCCACATCGTGGTGATC
  AGCAGTGTCATGGGCCTGCAGGGTGTCATCTTCAACGATGTCTATGCAGCTTCCAAGTTCGCCCTGGAGG
  GATTCTTCGAAAGCCTCGCTATCCAGCTGCTGCAGTTCAACATCTTCATCTCCCTGGTGGAGCCAGGCCC
  CGTGGTCACCGAGTTTGAGGGGAAGCTTCTGGCGCAGGTTTCTATGGCTGAGTTCCCAGGCACTGACCCT
  GAGACCCTGCACTACTTCCGGGACCTCTATCTCCCAGCCTCCAGGAAGCTGTTTTGCTCCGTGGGACAGA
  ACCCACAGGACGTGGTTCAGGCCATTGTCAACGTCATCAGCTCGACTCGACCACCCCTGCGCCGACAGAC
  CAACATCCGCTACTCGCCGCTGACCACGCTCAAAACCGTGGATTCCTCTGGCAGCCTGTATGTGCGAACG
  ACCCACCGCCTCCTCTTCCGCTGTCCACGCCTCCTCAACCTTGGCCTTCAATGTCTGTCCTGCGGCTGCC
  TCCCAACGCGGGTGCGGCCAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223945 representing NM_015725
  Red=Cloning site Green=Tags(s)
  
  MAAAPRTVLISGCSSGIGLELAVQLAHDPKKRYQVVATMRDLGKKETLEAAAGEALGQTLTVAQLDVCSD
  ESVAQCLSCIQGEVDVLVNNAGMGLVGPLEGLSLAAMQNVFDTNFFGAVRLVKAVLPGMKRRRQGHIVVI
  SSVMGLQGVIFNDVYAASKFALEGFFESLAIQLLQFNIFISLVEPGPVVTEFEGKLLAQVSMAEFPGTDP
  ETLHYFRDLYLPASRKLFCSVGQNPQDVVQAIVNVISSTRPPLRRQTNIRYSPLTTLKTVDSSGSLYVRT
  THRLLFRCPRLLNLGLQCLSCGCLPTRVRPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015725
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015725.1, NP_056540.1
• RefSeq Size: 1714
• RefSeq ORF: 936
• Locus ID: 50700
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Retinol metabolism
• MW: 33.6 kDa
• Gene Summary: This gene encodes a member of the short-chain dehydrogenase/reductase family. The encoded protein catalyzes the reduction of all-trans-retinal to all-trans-retinol, the first reaction step of the rhodopsin regeneration pathway. This enzymatic reaction is the rate-limiting step in the visual cycle. [provided by RefSeq, Feb 2014]