ITPKB (NM_002221) Human Tagged ORF Clone

CAT#: RC223964

ITPKB (Myc-DDK-tagged)-Human inositol-trisphosphate 3-kinase B (ITPKB)

Product Images

Western blot validation of overexpression lysate (Cat# LY419459) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223964 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITPKB
• Synonyms: IP3-3KB; IP3K; IP3K-B; IP3KB; PIG37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223964 representing NM_002221
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGTACTGCTATGCGCTCAATAGCCTGGTGATCATGAATAGCGCCAACGAGATGAAGAGCGGCG
  GCGGCCCGGGGCCCAGTGGCAGCGAGACGCCCCCGCCCCCGAGGAGGGCAGTGCTGAGCCCCGGCAGCGT
  TTTCAGCCCCGGGAGAGGCGCCTCTTTCCTCTTCCCCCCAGCCGAGTCGCTGTCCCCCGAGGAGCCCCGG
  AGCCCCGGGGGCTGGCGGAGCGGCCGGCGCAGGCTGAATAGTAGCAGCGGCAGTGGCAGCGGCAGCAGCG
  GCAGTAGCGTGAGCAGCCCAAGTTGGGCTGGTCGCCTGCGAGGGGACCGGCAGCAGGTGGTGGCAGCCGG
  TACCCTCTCCCCGCCAGGGCCGGAGGAGGCCAAGAGGAAGCTGCGGATCTTGCAGCGCGAGTTGCAGAAC
  GTGCAGGTGAACCAGAAAGTGGGCATGTTTGAGGCGCACATCCAGGCACAGAGCTCCGCCATTCAAGCGC
  CCCGCAGCCCGCGTTTGGGCAGGGCTCGCTCGCCCTCCCCGTGCCCCTTCCGCAGCAGCAGTCAGCCCCC
  TGGAAGGGTCCTGGTTCAGGGCGCCCGGAGCGAGGAACGGAGGACAAAGTCCTGGGGGGAGCAATGTCCA
  GAGACTTCAGGAACCGACTCCGGGAGGAAAGGAGGGCCCAGCCTATGCTCCTCGCAGGTGAAGAAAGGAA
  TGCCACCTCTTCCCGGCCGGGCTGCCCCTACAGGATCAGAGGCTCAGGGTCCATCCGCTTTTGTAAGGAT
  GGAGAAGGGTATCCCTGCCAGTCCCCGCTGTGGCTCACCCACAGCTATGGAAATTGACAAAAGGGGCTCT
  CCTACCCCGGGAACTCGGAGCTGCCTAGCTCCCTCATTGGGGCTGTTCGGAGCTAGCTTAACGATGGCCA
  CGGAAGTGGCAGCGAGAGTTACATCCACTGGGCCACACCGTCCACAGGATCTTGCCCTCACTGAGCCGTC
  TGGGAGAGCCCGTGAGCTTGAGGACCTGCAGCCCCCAGAGGCCCTGGTGGAGAGGCAGGGGCAGTTTCTG
  GGCAGTGAGACAAGCCCAGCCCCAGAAAGGGGCGGGCCCCGCGATGGAGAACCCCCTGGGAAGATGGGGA
  AAGGATATCTGCCCTGTGGCATGCCGGGCTCTGGGGAGCCTGAAGTGGGCAAAAGGCCAGAGGAGACGAC
  TGTGAGCGTGCAAAGCGCAGAGTCCTCTGATTCCCTGAGCTGGTCCAGGCTGCCCAGGGCCCTGGCCTCC
  GTAGGCCCTGAGGAGGCCCGAAGTGGGGCCCCCGTGGGCGGGGGGCGTTGGCAGCTCTCCGACAGAGTGG
  AGGGAGGGTCCCCAACGCTGGGCTTGCTTGGGGGCAGCCCCTCAGCACAGCCGGGGACCGGGAATGTGGA
  GGCGGGAATTCCTTCTGGCAGAATGCTGGAGCCTTTGCCCTGTTGGGACGCTGCGAAAGATCTGAAAGAA
  CCTCAGTGCCCTCCTGGGGACAGGGTGGGTGTGCAGCCTGGGAACTCCAGGGTTTGGCAGGGCACCATGG
  AGAAAGCCGGTTTGGCTTGGACGCGTGGCACAGGGGTGCAATCAGAGGGGACTTGGGAAAGCCAGCGGCA
  GGACAGTGATGCCCTCCCAAGTCCGGAGCTGCTACCCCAAGATCAGGACAAGCCTTTCCTGAGGAAGGCC
  TGCAGCCCCAGCAACATACCTGCTGTCATCATTACAGACATGGGCACCCAGGAGGATGGGGCCTTGGAGG
  AGACGCAGGGAAGCCCTCGGGGCAACCTGCCCCTGAGGAAACTGTCCTCTTCCTCGGCCTCCTCCACGGG
  CTTCTCCTCATCCTACGAAGACTCAGAGGAGGACATCTCCAGTGACCCTGAGCGCACCCTGGACCCCAAC
  TCAGCCTTCCTGCATACCCTGGACCAGCAGAAACCTAGAGTGAGCAAATCATGGAGGAAGATAAAAAACA
  TGGTGCACTGGTCTCCCTTCGTCATGTCCTTCAAGAAGAAGTACCCCTGGATCCAGCTGGCAGGACACGC
  AGGGAGTTTCAAGGCAGCTGCCAATGGCAGGATCCTGAAGAAGCACTGTGAGTCAGAGCAGCGCTGCCTG
  GACCGGCTGATGGTGGATGTGCTGAGGCCCTTCGTACCTGCCTACCATGGGGATGTGGTGAAGGACGGGG
  AGCGCTACAACCAGATGGACGACCTGCTGGCCGACTTCGACTCGCCCTGTGTGATGGACTGCAAGATGGG
  AATCAGGACCTACCTGGAGGAGGAGCTCACGAAGGCCCGGAAGAAGCCCAGCCTGCGGAAGGACATGTAC
  CAGAAGATGATCGAGGTGGACCCCGAGGCCCCCACCGAGGAGGAAAAAGCACAGCGGGCTGTGACCAAGC
  CACGGTACATGCAGTGGCGGGAGACCATCAGCTCCACGGCCACCCTGGGGTTCAGGATCGAGGGAATCAA
  GAAAGAAGACGGCACCGTGAACCGGGACTTCAAGAAGACCAAAACGAGGGAGCAGGTCACCGAGGCCTTC
  AGAGAGTTCACTAAAGGAAACCATAACATCCTGATCGCCTATCGGGACCGGCTGAAGGCCATTCGAACCA
  CTCTAGAAGTTTCTCCCTTCTTCAAGTGCCACGAGGTCATTGGCAGCTCCCTCCTCTTCATCCACGACAA
  GAAGGAACAGGCCAAAGTGTGGATGATCGACTTTGGGAAAACCACGCCCCTGCCTGAGGGCCAGACCCTG
  CAGCATGACGTCCCCTGGCAGGAGGGGAACCGGGAGGATGGCTACCTCTCGGGGCTCAATAACCTCGTCG
  ACATCCTGACCGAGATGTCCCAGGATGCCCCACTCGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223964 representing NM_002221
  Red=Cloning site Green=Tags(s)
  
  MAVYCYALNSLVIMNSANEMKSGGGPGPSGSETPPPPRRAVLSPGSVFSPGRGASFLFPPAESLSPEEPR
  SPGGWRSGRRRLNSSSGSGSGSSGSSVSSPSWAGRLRGDRQQVVAAGTLSPPGPEEAKRKLRILQRELQN
  VQVNQKVGMFEAHIQAQSSAIQAPRSPRLGRARSPSPCPFRSSSQPPGRVLVQGARSEERRTKSWGEQCP
  ETSGTDSGRKGGPSLCSSQVKKGMPPLPGRAAPTGSEAQGPSAFVRMEKGIPASPRCGSPTAMEIDKRGS
  PTPGTRSCLAPSLGLFGASLTMATEVAARVTSTGPHRPQDLALTEPSGRARELEDLQPPEALVERQGQFL
  GSETSPAPERGGPRDGEPPGKMGKGYLPCGMPGSGEPEVGKRPEETTVSVQSAESSDSLSWSRLPRALAS
  VGPEEARSGAPVGGGRWQLSDRVEGGSPTLGLLGGSPSAQPGTGNVEAGIPSGRMLEPLPCWDAAKDLKE
  PQCPPGDRVGVQPGNSRVWQGTMEKAGLAWTRGTGVQSEGTWESQRQDSDALPSPELLPQDQDKPFLRKA
  CSPSNIPAVIITDMGTQEDGALEETQGSPRGNLPLRKLSSSSASSTGFSSSYEDSEEDISSDPERTLDPN
  SAFLHTLDQQKPRVSKSWRKIKNMVHWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCL
  DRLMVDVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGIRTYLEEELTKARKKPSLRKDMY
  QKMIEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGTVNRDFKKTKTREQVTEAF
  REFTKGNHNILIAYRDRLKAIRTTLEVSPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTL
  QHDVPWQEGNREDGYLSGLNNLVDILTEMSQDAPLA
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002221
• ORF Size: 2838 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002221.2, NM_002221.3, NP_002212.2, NP_002212.3
• RefSeq Size: 5875 bp
• RefSeq ORF: 2841 bp
• Locus ID: 3707
• Cytogenetics: 1q42.12
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 102.2 kDa
• Gene Summary: 'The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. [provided by RefSeq, Jul 2008]'