ITPKB (NM_002221) Human Untagged Clone

CAT#: SC303151

ITPKB (untagged)-Human inositol-trisphosphate 3-kinase B (ITPKB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ITPKB
• Synonyms: IP3-3KB; IP3K; IP3K-B; IP3KB; PIG37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002221, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGTGTACTGCTATGCGCTCAATAGCCTGGTGATCATGAATAGCGCCAACGAGATGAAGAGCGGCG
  GCGGCCCGGGGCCCAGTGGCAGCGAGACGCCCCCGCCCCCGAGGAGGGCAGTGCTGAGCCCCGGCAGCGT
  TTTCAGCCCCGGGAGAGGCGCCTCTTTCCTCTTCCCCCCAGCCGAGTCGCTGTCCCCCGAGGAGCCCCGG
  AGCCCCGGGGGCTGGCGGAGCGGCCGGCGCAGGCTGAATAGTAGCAGCGGCAGTGGCAGCGGCAGCAGCG
  GCAGTAGCGTGAGCAGCCCAAGTTGGGCTGGTCGCCTGCGAGGGGACCGGCAGCAGGTGGTGGCAGCCGG
  TACCCTCTCCCCGCCAGGGCCGGAGGAGGCCAAGAGGAAGCTGCGGATCTTGCAGCGCGAGTTGCAGAAC
  GTGCAGGTGAACCAGAAAGTGGGCATGTTTGAGGCGCACATCCAGGCACAGAGCTCCGCCATTCAAGCGC
  CCCGCAGCCCGCGTTTGGGCAGGGCTCGCTCGCCCTCCCCGTGCCCCTTCCGCAGCAGCAGTCAGCCCCC
  TGGAAGGGTCCTGGTTCAGGGCGCCCGGAGCGAGGAACGGAGGACAAAGTCCTGGGGGGAGCAATGTCCA
  GAGACTTCAGGAACCGACTCCGGGAGGAAAGGAGGGCCCAGCCTATGCTCCTCGCAGGTGAAGAAAGGAA
  TGCCACCTCTTCCCGGCCGGGCTGCCCCTACAGGATCAGAGGCTCAGGGTCCATCCGCTTTTGTAAGGAT
  GGAGAAGGGTATCCCTGCCAGTCCCCGCTGTGGCTCACCCACAGCTATGGAAATTGACAAAAGGGGCTCT
  CCTACCCCGGGAACTCGGAGCTGCCTAGCTCCCTCATTGGGGCTGTTCGGAGCTAGCTTAACGATGGCCA
  CGGAAGTGGCAGCGAGAGTTACATCCACTGGGCCACACCGTCCACAGGATCTTGCCCTCACTGAGCCGTC
  TGGGAGAGCCCGTGAGCTTGAGGACCTGCAGCCCCCAGAGGCCCTGGTGGAGAGGCAGGGGCAGTTTCTG
  GGCAGTGAGACAAGCCCAGCCCCAGAAAGGGGCGGGCCCCGCGATGGAGAACCCCCTGGGAAGATGGGGA
  AAGGATATCTGCCCTGTGGCATGCCGGGCTCTGGGGAGCCTGAAGTGGGCAAAAGGCCAGAGGAGACGAC
  TGTGAGCGTGCAAAGCGCAGAGTCCTCTGATTCCCTGAGCTGGTCCAGGCTGCCCAGGGCCCTGGCCTCC
  GTAGGCCCTGAGGAGGCCCGAAGTGGGGCCCCCGTGGGCGGGGGGCGTTGGCAGCTCTCCGACAGAGTGG
  AGGGAGGGTCCCCAACGCTGGGCTTGCTTGGGGGCAGCCCCTCAGCACAGCCGGGGACCGGGAATGTGGA
  GGCGGGAATTCCTTCTGGCAGAATGCTGGAGCCTTTGCCCTGTTGGGACGCTGCGAAAGATCTGAAAGAA
  CCTCAGTGCCCTCCTGGGGACAGGGTGGGTGTGCAGCCTGGGAACTCCAGGGTTTGGCAGGGCACCATGG
  AGAAAGCCGGTTTGGCTTGGACGCGTGGCACAGGGGTGCAATCAGAGGGGACTTGGGAAAGCCAGCGGCA
  GGACAGTGATGCCCTCCCAAGTCCGGAGCTGCTACCCCAAGATCCGGACAAGCCTTTCCTGAGGAAGGCC
  TGCAGCCCCAGCAACATACCTGCTGTCATCATTACAGACATGGGCACCCAGGAGGATGGGGCCTTGGAGG
  AGACGCAGGGAAGCCCTCGGGGCAACCTGCCCCTGAGGAAACTGTCCTCTTCCTCGGCCTCCTCCACGGG
  CTTCTCCTCATCCTACGAAGACTCAGAGGAGGACATCTCCAGTGACCCTGAGCGCACCCTGGACCCCAAC
  TCAGCCTTCCTGCATACCCTGGACCAGCAGAAACCTAGAGTGAGCAAATCATGGAGGAAGATAAAAAACA
  TGGTGCACTGGTCTCCCTTCGTCATGTCCTTCAAGAAGAAGTACCCCTGGATCCAGCTGGCAGGACACGC
  AGGGAGTTTCAAGGCAGCTGCCAATGGCAGGATCCTGAAGAAGCACTGTGAGTCAGAGCAGCGCTGCCTG
  GACCGGCTGATGGTGGATGTGCTGAGGCCCTTCGTACCTGCCTACCATGGGGATGTGGTGAAGGACGGGG
  AGCGCTACAACCAGATGGACGACCTGCTGGCCGACTTCGACTCGCCCTGTGTGATGGACTGCAAGATGGG
  AATCAGGACCTACCTGGAGGAGGAGCTCACGAAGGCCCGGAAGAAGCCCAGCCTGCGGAAGGACATGTAC
  CAGAAGATGATCGAGGTGGACCCCGAGGCCCCCACCGAGGAGGAAAAAGCACAGCGGGCTGTGACCAAGC
  CACGGTACATGCAGTGGCGGGAGACCATCAGCTCCACGGCCACCCTGGGGTTCAGGATCGAGGGAATCAA
  GAAAGAAGACGGCACCGTGAACCGGGACTTCAAGAAGACCAAAACGAGGGAGCAGGTCACCGAGGCCTTC
  AGAGAGTTCACTAAAGGAAACCATAACATCCTGATCGCCTATCGGGACCGGCTGAAGGCCATTCGAACCA
  CTCTAGAAGTTTCTCCCTTCTTCAAGTGCCACGAGGTCATTGGCAGCTCCCTCCTCTTCATCCACGACAA
  GAAGGAACAGGCCAAAGTGTGGATGATCGACTTTGGGAAAACCACGCCCCTGCCTGAGGGCCAGACCCTG
  CAGCATGACGTCCCCTGGCAGGAGGGGAACCGGGAGGATGGCTACCTCTCGGGGCTCAATAACCTCGTCG
  ACATCCTGACCGAGATGTCCCAGGATGCCCCACTCGCCTGA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_002221
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002221.3, NP_002212.3
• RefSeq Size: 6162 bp
• RefSeq ORF: 2841 bp
• Locus ID: 3707
• Cytogenetics: 1q42.12
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: 'The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. [provided by RefSeq, Jul 2008]'