NKCC1 (SLC12A2) (NM_001046) Human Tagged ORF Clone
CAT#: RC224053
SLC12A2 (Myc-DDK-tagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2)
"NM_001046" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SLC12A2 |
Synonyms | BSC; BSC2; NKCC1; PPP1R141 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224053 representing NM_001046
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCGGCCCACGGCGCCCTCCTCCGGCGCCCCGGGACTGGCCGGGGTCGGGGAGACGCCGTCAG CCGCTGCGCTGGCCGCAGCCAGGGTGGAACTGCCCGGCACGGCTGTGCCCTCGGTGCCGGAGGATGCTGC GCCCGCGAGCCGGGACGGCGGCGGGGTCCGCGATGAGGGCCCCGCGGCGGCCGGGGACGGGCTGGGCAGA CCCTTGGGGCCCACCCCGAGCCAGAGCCGTTTCCAGGTGGACCTGGTTTCCGAGAACGCCGGGCGGGCCG CTGCTGCGGCGGCGGCGGCGGCGGCGGCAGCGGCGGCGGCTGGTGCTGGGGCGGGGGCCAAGCAGACCCC CGCGGACGGGGAAGCCAGCGGCGAGAGCGAGCCGGCTAAAGGCAGCGAGGAAGCCAAGGGCCGCTTCCGC GTGAACTTCGTGGACCCAGCTGCCTCCTCGTCGGCTGAAGACAGCCTGTCAGATGCTGCCGGGGTCGGAG TCGACGGGCCCAACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTC CGGCGGCGGCGGCGGCAGTGGGCACCACCAGCACTACTATTATGATACCCACACCAACACCTACTACCTG CGCACCTTCGGCCACAACACCATGGACGCTGTGCCCAGGATCGATCACTACCGGCACACAGCCGCGCAGC TGGGCGAGAAGCTGCTCCGGCCTAGCCTGGCGGAGCTCCACGACGAGCTGGAAAAGGAACCTTTTGAGGA TGGCTTTGCAAATGGGGAAGAAAGTACTCCAACCAGAGATGCTGTGGTCACGTATACTGCAGAAAGTAAA GGAGTCGTGAAGTTTGGCTGGATCAAGGGTGTATTAGTACGTTGTATGTTAAACATTTGGGGTGTGATGC TTTTCATTAGATTGTCATGGATTGTGGGTCAAGCTGGAATAGGTCTATCAGTCCTTGTAATAATGATGGC CACTGTTGTGACAACTATCACAGGATTGTCTACTTCAGCAATAGCAACTAATGGATTTGTAAGAGGAGGA GGAGCATATTATTTAATATCTAGAAGTCTAGGGCCAGAATTTGGTGGTGCAATTGGTCTAATCTTCGCCT TTGCCAACGCTGTTGCAGTTGCTATGTATGTGGTTGGATTTGCAGAAACCGTGGTGGAGTTGCTTAAGGA ACATTCCATACTTATGATAGATGAAATCAATGATATCCGAATTATTGGAGCCATTACAGTCGTGATTCTT TTAGGTATCTCAGTAGCTGGAATGGAGTGGGAAGCAAAAGCTCAGATTGTTCTTTTGGTGATCCTACTTC TTGCTATTGGTGATTTCGTCATAGGAACATTTATCCCACTGGAGAGCAAGAAGCCAAAAGGGTTTTTTGG TTATAAATCTGAAATATTTAATGAGAACTTTGGGCCCGATTTTCGAGAGGAAGAGACTTTCTTTTCTGTA TTTGCCATCTTTTTTCCTGCTGCAACTGGTATTCTGGCTGGAGCAAATATCTCAGGTGATCTTGCAGATC CTCAGTCAGCCATACCCAAAGGAACACTCCTAGCCATTTTAATTACTACATTGGTTTACGTAGGAATTGC AGTATCTGTAGGTTCTTGTGTTGTTCGAGATGCCACTGGAAACGTTAATGACACTATCGTAACAGAGCTA ACAAACTGTACTTCTGCAGCCTGCAAATTAAACTTTGATTTTTCATCTTGTGAAAGCAGTCCTTGTTCCT ATGGCCTAATGAACAACTTCCAGGTAATGAGTATGGTGTCAGGATTTACACCACTAATTTCTGCAGGTAT ATTTTCAGCCACTCTTTCTTCAGCATTAGCATCCCTAGTGAGTGCTCCCAAAATATTTCAGGCTCTATGT AAGGACAACATCTACCCAGCTTTCCAGATGTTTGCTAAAGGTTATGGGAAAAATAATGAACCTCTTCGTG GCTACATCTTAACATTCTTAATTGCACTTGGATTCATCTTAATTGCTGAACTGAATGTTATTGCACCAAT TATCTCAAACTTCTTCCTTGCATCATATGCATTGATCAATTTTTCAGTATTCCATGCATCACTTGCAAAA TCTCCAGGATGGCGTCCTGCATTCAAATACTACAACATGTGGATATCACTTCTTGGAGCAATTCTTTGTT GCATAGTAATGTTCGTCATTAACTGGTGGGCTGCATTGCTAACATATGTGATAGTCCTTGGGCTGTATAT TTATGTTACCTACAAAAAACCAGATGTGAATTGGGGATCCTCTACACAAGCCCTGACTTACCTGAATGCA CTGCAGCATTCAATTCGTCTTTCTGGAGTGGAAGACCACGTGAAAAACTTTAGGCCACAGTGTCTTGTTA TGACAGGTGCTCCAAACTCACGTCCAGCTTTACTTCATCTTGTTCATGATTTCACAAAAAATGTTGGTTT GATGATCTGTGGCCATGTACATATGGGTCCTCGAAGACAAGCCATGAAAGAGATGTCCATCGATCAAGCC AAATATCAGCGATGGCTTATTAAGAACAAAATGAAGGCATTTTATGCTCCAGTACATGCAGATGACTTGA GAGAAGGTGCACAGTATTTGATGCAGGCTGCTGGTCTTGGTCGTATGAAGCCAAACACACTTGTCCTTGG ATTTAAGAAAGATTGGTTGCAAGCAGATATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGCT TTTGACATACAATATGGAGTAGTGGTTATTCGCCTAAAAGAAGGTCTGGATATATCTCATCTTCAAGGAC AAGAAGAATTATTGTCATCACAAGAGAAATCTCCTGGCACCAAGGATGTGGTAGTAAGTGTGGAATATAG TAAAAAGTCCGATTTAGATACTTCCAAACCACTCAGTGAAAAACCAATTACACACAAAGTTGAGGAAGAG GATGGCAAGACTGCAACTCAACCACTGTTGAAAAAAGAATCCAAAGGCCCTATTGTGCCTTTAAATGTAG CTGACCAAAAGCTTCTTGAAGCTAGTACACAGTTTCAGAAAAAACAAGGAAAGAATACTATTGATGTCTG GTGGCTTTTTGATGATGGAGGTTTGACCTTATTGATACCTTACCTTCTGACGACCAAGAAAAAATGGAAA GACTGTAAGATCAGAGTATTCATTGGTGGAAAGATAAACAGAATAGACCATGACCGGAGAGCGATGGCTA CTTTGCTTAGCAAGTTCCGGATAGACTTTTCTGATATCATGGTTCTAGGAGATATCAATACCAAACCAAA GAAAGAAAATATTATAGCTTTTGAGGAAATCATTGAGCCATACAGACTTCATGAAGATGATAAAGAGCAA GATATTGCAGATAAAATGAAAGAAGATGAACCATGGCGAATAACAGATAATGAGCTTGAACTTTATAAGA CCAAGACATACCGGCAGATCAGGTTAAATGAGTTATTAAAGGAACATTCAAGCACAGCTAATATTATTGT CATGAGTCTCCCAGTTGCACGAAAAGGTGCTGTGTCTAGTGCTCTCTACATGGCATGGTTAGAAGCTCTA TCTAAGGACCTACCACCAATCCTCCTAGTTCGTGGGAATCATCAGAGTGTCCTTACCTTCTATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224053 representing NM_001046
Red=Cloning site Green=Tags(s) MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPEDAAPASRDGGGVRDEGPAAAGDGLGR PLGPTPSQSRFQVDLVSENAGRAAAAAAAAAAAAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFR VNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYL RTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESK GVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGG GAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVIL LGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSV FAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTEL TNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALC KDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAK SPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNA LQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQA KYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDA FDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEE DGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWK DCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQ DIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEAL SKDLPPILLVRGNHQSVLTFYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001046 |
ORF Size | 3636 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001046.1, NM_001046.2, NP_001037.1 |
RefSeq Size | 6891 bp |
RefSeq ORF | 3639 bp |
Locus ID | 6558 |
Cytogenetics | 5q23.3 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Vibrio cholerae infection |
MW | 131.3 kDa |
Gene Summary | 'The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC302962 | SLC12A2 (untagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2) |
USD 2,460.00 |
|
RG224053 | SLC12A2 (GFP-tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2) |
USD 1,400.00 |
|
RC224053L3 | Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2), Myc-DDK-tagged |
USD 1,470.00 |
|
RC224053L4 | Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2), mGFP tagged |
USD 1,470.00 |
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