NKCC1 (SLC12A2) (NM_001046) Human Untagged Clone

CAT#: SC302962

SLC12A2 (untagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC12A2
• Synonyms: BSC; BSC2; NKCC1; PPP1R141
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001046 edited
  GGGGGCCTCTGTGGCCGTCCAGGCTAGCGGCGGCCCGCAGGCGGCGGGGAGAAAGACTCT
  CTCACCTGGTCTTGCGGCTGTGGCCACCGCCGGCCAGGGGTGTGGAGGGCGTGCTGCCGG
  AGACGTCCGCCGGGCTCTGCAGTTCCGCCGGGGGTCGGGCAGCTATGGAGCCGCGGCCCA
  CGGCGCCCTCCTCCGGCGCCCCGGGACTGGCCGGGGTCGGGGAGACGCCGTCAGCCGCTG
  CGCTGGCCGCAGCCAGGGTGGAACTGCCCGGCACGGCTGTGCCCTCGGTGCCGGAGGATG
  CTGCGCCCGCGAGCCGGGACGGCGGCGGGGTCCGCGATGAGGGCCCCGCGGCGGCCGGGG
  ACGGGCTGGGCAGACCCTTGGGGCCCACCCCGAGCCAGAGCCGTTTCCAGGTGGACCTGG
  TTTCCGAGAACGCCGGGCGGGCCGCTGCTGCGGCGGCGGCGGCGGCGGCGGCAGCGGCGG
  CGGCTGGTGCTGGGGCGGGGGCCAAGCAGACCCCCGCGGACGGGGAAGCCAGCGGCGAGA
  GCGAGCCGGTTAAAGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTGGACC
  CAGCTGCCTCCTCGTCGGCTGAAGACAGCCTGTCAGATGCTGCCGGGGTCGGAGTCGACG
  GGCCCAACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGC
  ACTCCGGCGGCGGCGGCGGCAGTGGGCACCACCAGCACTACTATTATGATACCCACACCA
  ACACCTACTACCTGCGCACCTTCGGCCACAACACCATGGACGCTGTGCCCAGGATCGATC
  ACTACCGGCACACAGCCGCGCAGCTGGGCGAGAAGCTGCTCCGGCCTAGCCTGGCGGAGC
  TCCACGACGAGCTGGAAAAGGAACCTTTTGAGGATGGCTTTGCAAATGGGGAAGAAAGTA
  CTCCAACCAGAGATGCTGTGGTCACGTATACTGCAGAAAGTAAAGGAGTCGTGAAGTTTG
  GCTGGATCAAGGGTGTATTAGTACGTTGTATGTTAAACATTTGGGGTGTGATGCTTTTCA
  TTAGATTGTCATGGATTGTGGGTCAAGCTGGAATAGGTCTATCAGTCCTTGTAATAATGA
  TGGCCACTGTTGTGACAACTATCACAGGATTGTCTACTTCAGCAATAGCAACTAATGGAT
  TTGTAAGAGGAGGAGGAGCATATTATTTAATATCTAGAAGTCTAGGGCCAGAATTTGGTG
  GTGCAATTGGTCTAATCTTCGCCTTTGCCAACGCTGTTGCAGTTGCTATGTATGTGGTTG
  GATTTGCAGAAACCGTGGTGGAGTTGCTTAAGGAACATTCCATACTTATGATAGATGAAA
  TCAATGATATCCGAATTATTGGAGCCATTACAGTCGTGATTCTTTTAGGTATCTCAGTAG
  CTGGAATGGAGTGGGAAGCAAAAGCTCAGATTGTTCTTTTGGTGATCCTACTTCTTGCTA
  TTGGTGATTTCGTCATAGGAACATTTATCCCACTGGAGAGCAAGAAGCCAAAAGGGTTTT
  TTGGTTATAAATCTGAAATATTTAATGAGAACTTTGGGCCCGATTTTCGAGAGGAAGAGA
  CTTTCTTTTCTGTATTTGCCATCTTTTTTCCTGCTGCAACTGGTATTCTGGCTGGAGCAA
  ATATCTCAGGTGATCTTGCAGATCCTCAGTCAGCCATACCCAAAGGAACACTCCTAGCCA
  TTTTAATTACTACATTGGTTTACGTAGGAATTGCAGTATCTGTAGGTTCTTGTGTTGTTC
  GAGATGCCACTGGAAACGTTAATGACACTATCGTAACAGAGCTAACAAACTGTACTTCTG
  CAGCCTGCAAATTAAACTTTGATTTTTCATCTTGTGAAAGCAGTCCTTGTTCCTATGGCC
  TAATGAACAACTTCCAGGTAATGAGTATGGTGTCAGGATTTACACCACTAATTTCTGCAG
  GTATATTTTCAGCCACTCTTTCTTCAGCATTAGCATCCCTAGTGAGTGCTCCCAAAATAT
  TTCAGGCTCTATGTAAGGACAACATCTACCCAGCTTTCCAGATGTTTGCTAAAGGTTATG
  GGAAAAATAATGAACCTCTTCGTGGCTACATCTTAACATTCTTAATTGCACTTGGATTCA
  TCTTAATTGCTGAACTGAATGTTATTGCACCAATTATCTCAAACTTCTTCCTTGCATCAT
  ATGCATTGATCAATTTTTCAGTATTCCATGCATCACTTGCAAAATCTCCAGGATGGCGTC
  CTGCATTCAAATACTACAACATGTGGATATCACTTCTTGGAGCAATTCTTTGTTGCATAG
  TAATGTTCGTCATTAACTGGTGGGCTGCATTGCTAACATATGTGATAGTCCTTGGGCTGT
  ATATTTATGTTACCTACAAAAAACCAGATGTGAATTGGGGATCCTCTACACAAGCCCTGA
  CTTACCTGAATGCACTGCAGCATTCAATTCGTCTTTCTGGAGTGGAAGACCACGTGAAAA
  ACTTTAGGCCACAGTGTCTTGTTATGACAGGTGCTCCAAACTCACGTCCAGCTTTACTTC
  ATCTTGTTCATGATTTCACAAAAAATGTTGGTTTGATGATCTGTGGCCATGTACATATGG
  GTCCTCGAAGACAAGCCATGAAAGAGATGTCCATCGATCAAGCCAAATATCAGCGATGGC
  TTATTAAGAACAAAATGAAGGCATTTTATGCTCCAGTACATGCAGATGACTTGAGAGAAG
  GTGCACAGTATTTGATGCAGGCTGCTGGTCTTGGTCGTATGAAGCCAAACACACTTGTCC
  TTGGATTTAAGAAAGATTGGTTGCAAGCAGATATGAGGGATGTGGATATGTATATAAACT
  TATTTCATGATGCTTTTGACATACAATATGGAGTAGTGGTTATTCGCCTAAAAGAAGGTC
  TGGATATATCTCATCTTCAAGGACAAGAAGAATTATTGTCATCACAAGAGAAATCTCCTG
  GCACCAAGGATGTGGTAGTAAGTGTGGAATATAGTAAAAAGTCCGATTTAGATACTTCCA
  AACCACTCAGTGAAAAACCAATTACACACAAAGTTGAGGAAGAGGATGGCAAGACTGCAA
  CTCAACCACTGTTGAAAAAAGAATCCAAAGGCCCTATTGTGCCTTTAAATGTAGCTGACC
  AAAAGCTTCTTGAAGCTAGTACACAGTTTCAGAAAAAACAAGGAAAGAATACTATTGATG
  TCTGGTGGCTTTTTGATGATGGAGGTTTGACCTTATTGATACCTTACCTTCTGACGACCA
  AGAAAAAATGGAAAGACTGTAAGATCAGAGTATTCATTGGTGGAAAGATAAACAGAATAG
  ACCATGACCGGAGAGCGATGGCTACTTTGCTTAGCAAGTTCCGGATAGACTTTTCTGATA
  TCATGGTTCTAGGAGATATCAATACCAAACCAAAGAAAGAAAATATTATAGCTTTTGAGG
  AAATCATTGAGCCATACAGACTTCATGAAGATGATAAAGAGCAAGATATTGCAGATAAAA
  TGAAAGAAGATGAACCATGGCGAATAACAGATAATGAGCTTGAACTTTATAAGACCAAGA
  CATACCGGCAGATCAGGTTAAATGAGTTATTAAAGGAACATTCAAGCACAGCTAATATTA
  TTGTCATGAGTCTCCCAGTTGCACGAAAAGGTGCTGTGTCTAGTGCTCTCTACATGGCAT
  GGTTAGAAGCTCTATCTAAGGACCTACCACCAATCCTCCTAGTTCGTGGGAATCATCAGA
  GTGTCCTTACCTTCTATTCATAAATGTTCTATACAGTGGACAGCCCTCCAGAATGGTACT
  TCAGTGCCTAGTGTAGTAACTGAAATCTTCAATGACACATTAACATCACAATGGCGAATG
  GTGACTTTTCTTTCACGATTTCATTAATTTGAAAGCACACAGGAAAGTTGCTCCATTGAT
  AACGTGTATGGAGACTTCGGTTTTAGTCAATTCCATATCTCAATCTTAATGGTGATTCTT
  CTCTGTTGAACTGAAGTTTGTGAGAGTAGTTTTCCTTTGCTACTTGAATAGCAATAAAAG
  CGTGTTAACTTTTTGATTGATGAAAGAAGTACAAAAAGCCTTTAGCCTTGAGGTGCCTTC
  TGAAATTAACCAAATTTCATCCATATATCCTCTTTTATAAACTTATAGAATGTCAAAAAA
  AAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001046
• Insert Size: 4200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001046.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001046.2, NP_001037.1
• RefSeq Size: 6891 bp
• RefSeq ORF: 3639 bp
• Locus ID: 6558
• Cytogenetics: 5q23.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection
• Gene Summary: 'The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).