MSH5 (NM_172165) Human Tagged ORF Clone
CAT#: RC224095L4
- LentiORF®
Lenti-ORF clone of MSH5 (mGFP-tagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 2
"NM_172165" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | MSH5 |
Synonyms | G7; MUTSH5; NG23; POF13 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC224095).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_172165 |
ORF Size | 2505 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172165.3, NP_751897.1 |
RefSeq Size | 2790 bp |
RefSeq ORF | 2508 bp |
Locus ID | 4439 |
Cytogenetics | 6p21.33 |
Protein Families | Druggable Genome |
MW | 93 kDa |
Gene Summary | 'This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4. Polymorphisms in this gene have been linked to various human diseases, including IgA deficiency, common variable immunodeficiency, and premature ovarian failure. Alternative splicing results multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 6 open reading frame 26 (C6orf26) gene. [provided by RefSeq, Feb 2011]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC309165 | MSH5 (untagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 2 |
USD 760.00 |
|
RC224095 | MSH5 (Myc-DDK-tagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 2 |
USD 880.00 |
|
RG224095 | MSH5 (GFP-tagged) - Human mutS homolog 5 (E. coli) (MSH5), transcript variant 2 |
USD 970.00 |
|
RC224095L3 | Lenti-ORF clone of MSH5 (Myc-DDK-tagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 2 |
USD 1,080.00 |
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