MSH5 (NM_172165) Human Untagged Clone

CAT#: SC309165

MSH5 (untagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSH5
• Synonyms: G7; MUTSH5; NG23; POF13
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_172165, the custom clone sequence may differ by one or more nucleotides
  ATGGCCTCCTTAGGAGCGAACCCAAGGAGGACACCGCAGGGACCGAGACCTGGGGCGGCC
  TCCTCCGGCTTCCCCAGCCCGGCCCCAGTGCCGGGCCCCAGGGAGGCCGAGGAGGAGGAA
  GTCGAGGAGGAGGAGGAGCTGGCCGAGATCCATCTGTGTGTGCTGTGGAATTCAGGATAC
  TTGGGCATTGCCTACTATGATACTAGTGACTCCACTATCCACTTCATGCCAGATGCCCCA
  GACCACGAGAGCCTCAAGCTTCTCCAGAGAGTTCTGGATGAGATCAATCCCCAGTCTGTT
  GTTACGAGTGCCAAACAGGATGAGAATATGACTCGATTTCTGGGAAAGCTTGCCTCCCAG
  GAGCACAGAGAGCCTAAAAGACCTGAAATCATATTTTTGCCAAGTGTGGATTTTGGTCTG
  GAGATAAGCAAACAACGCCTCCTTTCTGGAAACTACTCCTTCATCCCAGACGCCATGACT
  GCCACTGAGAAAATCCTCTTCCTCTCTTCCATTATTCCCTTTGACTGCCTCCTCACAGTT
  CGAGCACTTGGAGGGCTGCTGAAGTTCCTGGGTCGAAGAAGAATCGGGGTTGAACTGGAA
  GACTATAATGTCAGCGTCCCCATCCTGGGCTTTAAGAAATTTATGTTGACTCATCTGGTG
  AACATAGATCAAGACACTTACAGTGTTCTACAGATTTTTAAGAGTGAGTCTCACCCCTCA
  GTGTACAAAGTGGCCAGTGGACTGAAGGAGGGGCTCAGCCTCTTTGGAATCCTCAACAGA
  TGCCACTGTAAGTGGGGAGAGAAGCTGCTCAGGCTATGGTTCACACGTCCGACTCATGAC
  CTGGGGGAGCTCAGTTCTCGTCTGGACGTCATTCAGTTTTTTCTGCTGCCCCAGAATCTG
  GACATGGCTCAGATGCTGCATCGGCTCCTGGGTCACATCAAGAACGTGCCTCTGATTCTG
  AAACGCATGAAGTTGTCCCACACCAAGGTCAGCGACTGGCAGGTTCTCTACAAGACTGTG
  TACAGTGCCCTGGGCCTGAGGGATGCCTGCCGCTCCCTGCCGCAGTCCATCCAGCTCTTT
  CGGGACATTGCCCAAGAGTTCTCTGATGACCTGCACCATATCGCCAGCCTCATTGGGAAA
  GTAGTGGACTTTGAGGGCAGCCTTGCTGAAAATCGCTTCACAGTCCTCCCCAACATAGAT
  CCTGAAATTGATGAGAAAAAGCGAAGACTGATGGGACTTCCCAGTTTCCTTACTGAGGTT
  GCCCGCAAGGAGCTGGAGAATCTGGACTCCCGTATTCCTTCATGCAGTGTCATCTACATC
  CCTCTGATTGGCTTCCTTCTTTCTATTCCCCGCCTGCCTTCCATGGTAGAGGCCAGTGAC
  TTTGAGATTAATGGACTGGACTTCATGTTTCTCTCAGAGGAGAAGCTGCACTATCGTAGT
  GCCCGAACCAAGGAGCTGGATGCATTGCTGGGGGACCTGCACTGCGAGATCCGGGACCAG
  GAGACGCTGCTGATGTACCAGCTACAGTGCCAGGTGCTGGCACGAGCAGCTGTCTTAACC
  CGAGTATTGGACCTTGCCTCCCGCCTGGACGTCCTGCTGGCTCTTGCCAGTGCTGCCCGG
  GACTATGGCTACTCAAGGCCGCGTTACTCCCCACAAGTCCTTGGGGTACGAATCCAGAAT
  GGCAGACATCCTCTGATGGAACTCTGTGCCCGAACCTTTGTGCCCAACTCCACAGAATGT
  GGTGGGGACAAAGGGAGGGTCAAAGTCATCACTGGACCCAACTCATCAGGGAAGAGCATA
  TACCTCAAACAGGTAGGCTTGATCACATTCATGGCCCTGGTAGGCAGCTTTGTGCCAGCA
  GAGGAGGCCGAAATTGGGGCAGTAGACGCCATCTTCACACGAATTCATAGCTGCGAATCC
  ATCTCCCTTGGCCTCTCCACCTTCATGATCGACCTCAACCAGCAGGTGGCGAAAGCAGTG
  AACAATGCCACTGCACAGTCGCTGGTCCTTATTGATGAATTTGGAAAGGGAACCAACACG
  GTGGATGGGCTCGCGCTTCTGGCCGCTGTGCTCCGACACTGGCTGGCACGTGGACCCACA
  TGCCCCCACATCTTTGTGGCCACCAACTTTCTGAGCCTTGTTCAGCTACAACTGCTGCCA
  CAAGGGCCCCTGGTGCAGTATTTGACCATGGAGACCTGTGAGGATGGCAACGATCTTGTC
  TTCTTCTATCAGGTTTGCGAAGGTGTTGCGAAGGCCAGCCATGCCTCCCACACAGCTGCC
  CAGGCTGGGCTTCCTGACAAGCTTGTGGCTCGTGGCAAGGAGGTCTCAGACTTGATCCGC
  AGTGGAAAACCCATCAAGCCTGTCAAGGATTTGCTAAAGAAGAACCAAATGGAAAATTGC
  CAGACATTAGTGGATAAGTTTATGAAACTGGATTTGGAAGATCCTAACCTGGACTTGAAC
  GTTTTCATGAGCCAGGAAGTGCTGCCTGCTGCCACCAGCATCCTCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_172165
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172165.1, NP_751897.1
• RefSeq Size: 3971 bp
• RefSeq ORF: 2790 bp
• Locus ID: 4439
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4. Polymorphisms in this gene have been linked to various human diseases, including IgA deficiency, common variable immunodeficiency, and premature ovarian failure. Alternative splicing results multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 6 open reading frame 26 (C6orf26) gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (2) uses alternate in-frame splice sites in both the central and 3' coding regions, compared to variant 1, resulting in an isoform (b) that is longer than isoform a.