ENOX2 (NM_006375) Human Tagged ORF Clone

CAT#: RC224209

ENOX2 (Myc-DDK-tagged)-Human ecto-NOX disulfide-thiol exchanger 2 (ENOX2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY416684) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224209 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ENOX2 protein (Cat# TP324209). The protein was produced from HEK293T cells transfected with ENOX2 cDNA clone (Cat# RC224209) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ENOX2
• Synonyms: APK1; COVA1; tNOX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224209 representing NM_006375
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACTACCTATGTCTGATCCAACTGCATGGGCCACAGCAATGAATAATCTTGGAATGGCACCGCTGG
  GAATTGCCGGACAACCAATTTTACCTGACTTTGATCCTGCTCTTGGAATGATGACTGGAATTCCACCAAT
  AACTCCAATGATGCCTGGTTTGGGAATAGTACCTCCACCAATTCCTCCAGATATGCCAGTAGTAAAAGAG
  ATCATACACTGTAAAAGCTGCACGCTCTTCCCTCCAAATCCAAATCTCCCACCTCCTGCAACCCGAGAAA
  GACCACCAGGATGCAAAACAGTATTTGTGGGTGGTCTGCCTGAAAATGGGACAGAGCAAATCATTGTGGA
  AGTTTTCGAGCAGTGTGGAGAGATCATTGCCATTCGCAAGAGCAAGAAGAACTTCTGCCACATTCGCTTT
  GCTGAGGAGTACATGGTGGACAAAGCCCTGTATCTGTCTGGTTACCGCATTCGCCTGGGCTCTAGTACTG
  ACAAGAAGGACACAGGCAGACTCCACGTTGATTTCGCACAGGCTCGAGATGACCTGTATGAGTGGGAGTG
  TAAACAGCGTATGCTAGCCAGAGAGGAGCGCCATCGTAGAAGAATGGAAGAAGAAAGATTGCGTCCACCA
  TCTCCACCCCCAGTGGTCCACTATTCAGATCATGAATGCAGCATTGTTGCTGAAAAATTAAAAGATGATT
  CCAAATTCTCAGAAGCTGTACAGACCTTGCTTACCTGGATAGAGCGAGGAGAGGTCAACCGTCGTAGCGC
  CAATAACTTCTACTCCATGATCCAGTCGGCCAACAGCCATGTCCGCCGCCTGGTGAACGAGAAAGCTGCC
  CATGAGAAAGATATGGAAGAAGCAAAGGAGAAGTTCAAGCAGGCCCTTTCTGGAATTCTCATTCAATTTG
  AGCAGATAGTGGCTGTGTACCATTCCGCCTCCAAGCAGAAGGCATGGGACCACTTCACAAAAGCCCAGCG
  GAAGAACATCAGCGTGTGGTGCAAACAAGCTGAGGAAATTCGCAACATTCATAATGATGAATTAATGGGA
  ATCAGGCGAGAAGAAGAAATGGAAATGTCTGATGATGAAATAGAAGAAATGACAGAAACAAAAGAAACTG
  AGGAATCAGCCTTAGTATCACAGGCAGAAGCTCTGAAGGAAGAAAATGACAGCCTCCGTTGGCAGCTCGA
  TGCCTACCGGAATGAAGTAGAACTGCTCAAGCAAGAACAAGGCAAAGTCCACAGAGAAGATGACCCTAAC
  AAAGAACAGCAGCTGAAACTCCTGCAACAAGCCCTGCAAGGAATGCAACAGCATCTACTCAAAGTCCAAG
  AGGAATACAAAAAGAAAGAAGCTGAACTTGAAAAACTCAAAGATGACAAGTTACAGGTGGAAAAAATGTT
  GGAAAATCTTAAAGAAAAGGAAAGCTGTGCTTCTAGGCTGTGTGCCTCAAACCAGGATAGCGAATACCCT
  CTTGAGAAGACCATGAACAGCAGTCCTATCAAATCTGAACGTGAAGCACTGCTAGTGGGGATTATCTCCA
  CATTCCTTCATGTTCACCCATTTGGAGCAAGCATTGAATACATCTGTTCCTACTTGCACCGTCTTGATAA
  TAAGATCTGCACCAGCGATGTGGAGTGTCTCATGGGTAGACTCCAGCATACCTTCAAGCAGGAAATGACT
  GGAGTTGGAGCCAGCCTGGAAAAGAGATGGAAATTCTGTGGCTTCGAGGGCTTGAAGCTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224209 representing NM_006375
  Red=Cloning site Green=Tags(s)
  
  MTLPMSDPTAWATAMNNLGMAPLGIAGQPILPDFDPALGMMTGIPPITPMMPGLGIVPPPIPPDMPVVKE
  IIHCKSCTLFPPNPNLPPPATRERPPGCKTVFVGGLPENGTEQIIVEVFEQCGEIIAIRKSKKNFCHIRF
  AEEYMVDKALYLSGYRIRLGSSTDKKDTGRLHVDFAQARDDLYEWECKQRMLAREERHRRRMEEERLRPP
  SPPPVVHYSDHECSIVAEKLKDDSKFSEAVQTLLTWIERGEVNRRSANNFYSMIQSANSHVRRLVNEKAA
  HEKDMEEAKEKFKQALSGILIQFEQIVAVYHSASKQKAWDHFTKAQRKNISVWCKQAEEIRNIHNDELMG
  IRREEEMEMSDDEIEEMTETKETEESALVSQAEALKEENDSLRWQLDAYRNEVELLKQEQGKVHREDDPN
  KEQQLKLLQQALQGMQQHLLKVQEEYKKKEAELEKLKDDKLQVEKMLENLKEKESCASRLCASNQDSEYP
  LEKTMNSSPIKSEREALLVGIISTFLHVHPFGASIEYICSYLHRLDNKICTSDVECLMGRLQHTFKQEMT
  GVGASLEKRWKFCGFEGLKLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006375
• ORF Size: 1743 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006375.2, NM_006375.3, NP_006366.2
• RefSeq Size: 4095
• RefSeq ORF: 1746
• Locus ID: 10495
• Protein Families: Secreted Protein
• MW: 66.4 kDa
• Gene Summary: This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]