CDK5RAP2 (NM_001011649) Human Tagged ORF Clone

CAT#: RC224233

CDK5RAP2 (Myc-DDK-tagged)-Human CDK5 regulatory subunit associated protein 2 (CDK5RAP2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDK5RAP2
• Synonyms: C48; Cep215; MCPH3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224233 representing NM_001011649
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGACTTGGTGTTGGAAGAGGACGTCACCGTCCCTGGGACGCTCAGCGGCTGCAGTGGCCTTGTTC
  CCAGTGTACCAGATGACCTGGATGGCATCAACCCCAATGCTGGGTTGGGAAATGGTCTGCTCCCAAATGT
  GTCAGAAGAAACAGTGTCTCCCACCAGAGCACGGAACATGAAGGACTTTGAAAATCAAATCACTGAATTG
  AAGAAAGAAAACTTTAACCTAAAGCTCCGCATCTATTTCCTTGAGGAAAGAATGCAACAGGAATTTCATG
  GCCCCACTGAACATATCTACAAAACTAACATTGAGCTCAAGGTGGAAGTAGAAAGTCTGAAGCGGGAACT
  CCAGGAGAGAGAGCAGCTGCTCATCAAAGCCTCCAAAGCAGTTGAGAGCTTAGCTGAAGCAGGTGGCTCT
  GAAATCCAGCGGGTGAAAGAAGATGCTCGAAAGAAGGTGCAGCAGGTGGAAGATCTCCTAACTAAAAGAA
  TACTCCTTTTGGAAAAGGATGTGACAGCCGCCCAGGCAGAACTGGAAAAGGCCTTTGCAGGGACAGAGAC
  GGAGAAGGCTCTTCGGTTGCGTTTGGAAAGCAAGCTTTCAGAGATGAAGAAGATGCACGAGGGGGACTTG
  GCGATGGCTCTGGTCCTGGATGAGAAAGACAGACTGATTGAGGAGTTGAAGCTGTCTTTGAAGAGCAAAG
  AAGCTTTAATTCAGTGCCTTAAAGAGGAGAAATCTCAGATGGCATGTCCTGATGAGAATGTGTCATCTGG
  AGAGCTCCGAGGACTTTGTGCTGCTCCAAGGGAAGAAAAGGAGAGAGAAACTGAGGCTGCACAAATGGAG
  CATCAGAAGGAGAGAAACAGCTTTGAAGAGAGGATCCAGGCACTTGAAGAGGACCTGAGAGAGAAGGAAA
  GAGAAATTGCTACAGAGAAGAAAAATAGTCTAAAGAGGGATAAAGCCATTCAGGGTTTAACCATGGCATT
  AAAATCAAAGGAAAAAAAGGTTGAAGAACTTAACTCTGAAATTGAAAAGCTCAGTGCTGCCTTTGCTAAA
  GCCAGAGAGGCCCTACAGAAAGCACAGACCCAGGAATTTCAGGGGTCTGAAGACTATGAGACTGCTCTAT
  CAGGAAAGGAAGCCCTTTCGGCTGCGCTGCGCTCACAAAACCTCACCAAGAGTACAGAGAACCACAGACT
  GCGTAGAAGCATTAAGAAGATCACCCAGGAGCTGAGTGACTTGCAGCAGGAGAGGGAGAGACTGGAGAAG
  GACCTGGAGGAAGCCCATCGAGAGAAGAGCAAAGGAGACTGCACCATCCGTGATCTTAGAAATGAAGTTG
  AAAAATTACGCAATGAAGTGAATGAAAGAGAGAAAGCAATGGAAAATCGTTACAAGAGTCTTCTGAGTGA
  AAGCAATAAAAAATTGCACAATCAAGAGCAAGTGATCAAACATCTAACAGAAAGTACCAATCAGAAGGAC
  GTGTTGCTTCAGAAATTCAATGAAAAAGATTTGGAAGTAATACAGCAGAACTGCTATTTAATGGCTGCAG
  AGGATCTTGAGCTCAGGAGTGAAGGCTTAATAACAGAAAAGTGCTCTTCTCAACAGCCACCAGGCAGCAA
  AACCATCTTCTCTAAGGAAAAGAAACAATCATCAGACTATGAAGAGCTGATTCAGGTCTTAAAGAAAGAG
  CAGGACATCTATACCCATCTGGTCAAATCTCTGCAGGAATCAGACAGTATCAACAACCTGCAGGCTGAGT
  TAAACAAGATTTTTGCCCTGCGGAAGCAACTGGAGCAGGATGTGCTTTCATATCAGAATTTGCGGAAGAC
  CTTGGAGGAGCAGATCAGCGAAATTCGGAGGCGGGAAGAAGAATCATTTTCACTTTATAGTGATCAAACA
  TCTTATCTAAGTATTTGCCTTGAAGAAAACAATCGGTTTCAAGTGGAACATTTTTCTCAAGAAGAACTTA
  AGAAAAAGGTCAGTGACCTTATACAGCTAGTGAAGGAGCTGTATACAGACAACCAGCACCTGAAGAAAAC
  CATTTTTGATCTCTCCTGCATGGGTTTCCAGGGAAATGGGTTTCCAGATAGACTTGCGTCTACAGAACAA
  ACAGAGCTTCTGGCTAGCAAGGAGGACGAGGACACGATCAAAATTGGGGAGGATGACGAGATTAATTTCC
  TGAGTGACCAGCATTTGCAGCAGAGTAATGAGATTATGAAAGACCTTTCCAAAGGAGGCTGCAAAAATGG
  ATACTTAAGGCACACGGAGTCTAAGATTTCAGATTGTGATGGGGCCCACGCACCTGGCTGCCTAGAAGAA
  GGTGCATTCATAAACCTGCTTGCCCCTTTGTTCAATGAGAAGGCCACATTATTACTGGAATCCAGGCCAG
  ACCTTCTGAAAGTGGTACGGGAACTGCTTCTGGGACAACTATTCTTGACAGAGCAGGAAGTTTCTGGAGA
  ACACCTTGATGGTAAAACTGAGAAGACACCTAAGCAAAAAGGTGAACTTGTACATTTTGTCCAAACCAAC
  TCATTTTCCAAGCCACATGATGAACTGAAGTTGTCTTGTGAGGCCCAGCTAGTAAAGGCAGGCGAAGTGC
  CCAAGGTAGGACTGAAAGATGCCTCAGTGCAGACTGTGGCCACGGAGGGCGACCTGCTGAGATTCAAGCA
  TGAAGCAACAAGAGAGGCTTGGGAAGAGAAACCGATCAACACTGCACTCAGCGCAGAGCATCGGCCAGAG
  AACCTGCACGGGGTGCCTGGGTGGCAGGCTGCCCTCCTTTCCCTCCCTGGTATTACCAACAGAGAGGCTA
  AGAAGTCCCGCTTGCCAATCCTAATAAAACCATCCCGGTCATTAGGAAATATGTATCGTCTCCCTGCCAC
  CCAGGAGGTGGTGACGCAGCTGCAGAGCCAGATCTTGGAGCTGCAGGGGGAGCTGAAGGAGTTTAAAACT
  TGTAATAAGCAACTTCACCAAAAGTTAATTCTGGCTGAAGCAGTGATGGAGGGGAGGCCAACGCCCGACA
  AAACGTTGCTGAATGCTCAGCCCCCTGTGGGAGCAGCCTACCAGGACAGCCCAGGAGAGCAGAAAGGAAT
  TAAAACCACATCTTCTGTCTGGAGAGACAAGGAAATGGACAGTGATCAGCAAAGAAGCTACGAGATTGAC
  TCTGAGATTTGCCCACCTGATGACCTTGCCAGCTTGCCATCATGCAAAGAAAATCCTGAAGATGTTCTGA
  GCCCAACTTCAGTAGCTACTTACCTGAGTTCCAAGAGTCAGCCTTCTGCTAAAGTCAGTGTGATGGGGAC
  TGATCAGTCAGAGAGCATTAATACCTCAAATGAGACAGAATACTTAAAACAGAAAATCCATGACTTGGAA
  ACTGAGCTGGAAGGCTACCAGAATTTCATATTTCAGCTTCAAAAGCACTCCCAGTGCAGTGAGGCCATAA
  TTACAGTTTTGTGTGGGACAGAAGGGGCCCAGGATGGCTTGAGCAAGCCCAAGAATGGTTCTGATGGGGA
  AGAAATGACCTTTTCAAGTTTGCACCAAGTGCGATACGTGAAACACGTGAAAATCCTCGGTCCGCTGGCC
  CCAGAGATGATTGACAGCAGGGTGCTGGAGAACCTCAAACAGCAGCTGGAGGAACAGGAATACAAGCTGC
  AGAAGGAGCAGAATTTGAACATGCAACTTTTCAGTGAGATCCATAATCTGCAGAATAAGTTCAGAGATCT
  CTCACCTCCCAGATACGATTCATTAGTTCAGTCCCAAGCCAGGGAGCTCTCCCTTCAACGGCAGCAGATT
  AAGGATGGCCATGGCATCTGTGTCATCTCCCGTCAACACATGAACACCATGATTAAGGCATTTGAGGAGT
  TGCTGCAGGCCAGTGATGTGGATTACTGTGTGGCCGAGGGTTTCCAGGAACAGCTGAATCAATGTGCTGA
  GCTGCTGGAGAAATTGGAAAAGCTATTTCTCAACGGAAAATCAGTTGGAGTGGAAATGAACACCCAGAAT
  GAACTGATGGAGAGGATTGAGGAAGACAACTTAACCTACCAACATCTTCTGCCTGAATCTCCTGAGCCTT
  CAGCCTCTCATGCGCTCTCTGATTATGAAACATCTGAAAAGTCCTTCTTCTCACGAGACCAGAAGCAAGA
  TAATGAGACAGAGAAGACTTCAGTTATGGTGAACAGTTTTTCTCAAGACTTACTAATGGAACACATACAG
  GAAATTCGAACTTTGAGAAAGCGTTTAGAAGAATCTATTAAAACAAATGAGAAGCTACGGAAACAGTTGG
  AACGGCAAGGATCTGAATTTGTTCAAGGTTCTACAAGCATTTTTGCTTCTGGTTCAGAGCTTCATAGTTC
  TCTAACATCAGAAATTCATTTCTTGAGGAAGCAGAACCAGGCCCTCAATGCAATGCTCATTAAAGGATCC
  AGAGATAAACAGAAGGAGAATGACAAATTACGAGAGTCCCTCTCCAGGAAGACCGTGAGCCTGGAGCACC
  TTCAGCGGGAGTATGCCAGCGTGAAGGAAGAAAATGAAAGGCTGCAGAAAGAAGGCAGCGAGAAGGAGAG
  ACACAACCAGCAGCTGATCCAGGAGGTCCGCTGCAGCGGCCAGGAGCTGAGCAGGGTGCAGGAGGAGGTG
  AAGTTGAGGCAGCAGCTGCTCTCACAGAATGACAAGCTATTGCAGTCTCTCCGAGTGGAGCTGAAGGCGT
  ATGAGAAGCTGGATGAAGAGCACAGGAGACTGAGAGATGGTGACAAATATCCCATGGAAAGTGATAATTC
  ATTTGATCTGTTTGATTCCTCCCAGGCAGTGACACCAAAATCAGTTTCAGAGACTCCTCCACTCTCTGGG
  AATGACACGGACTCCCTCTCCTGCGACAGTGGCAGTTCGGCAACTAGCACTCCGTGTGTGTCCCGCCTGG
  TCACTGGCCACCACCTGTGGGCCAGCAAGAATGGCCGCCATGTCCTGGGCCTGATTGAGGACTATGAGGC
  CCTGCTCAAACAGATCAGCCAGGGACAGAGGCTCCTTGCTGAAATGGACATTCAAACCCAAGAGGCTCCC
  AGCTCCACAAGTCAAGAGCTGGGAACAAAGGGTCCACACCCAGCACCACTGAGCAAGTTTGTGAGCAGTG
  TGAGCACGGCCAAGCTGACCCTGGAAGAGGCCTACAGGCGGCTGAAGCTTCTCTGGAGAGTCTCACTCCC
  CGAGGATGGCCAGTGCCCCCTTCACTGTGAGCAGATTGGAGAAATGAAGGCAGAGGTCACCAAACTACAT
  AAAAAATTGTTTGAACAAGAAAAGAAGTTGCAAAACACCATGAAGCTTTTGCAGCTGAGCAAGCGCCAGG
  AAAAAGTCATCTTTGATCAATTGGTCGTAACCCACAAAATCCTTCGGAAGGCCAGAGGAAACCTGGAGCT
  TAGGCCTGGGGGAGCCCATCCAGGAACATGCAGTCCCAGCAGACCAGGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224233 representing NM_001011649
  Red=Cloning site Green=Tags(s)
  
  MMDLVLEEDVTVPGTLSGCSGLVPSVPDDLDGINPNAGLGNGLLPNVSEETVSPTRARNMKDFENQITEL
  KKENFNLKLRIYFLEERMQQEFHGPTEHIYKTNIELKVEVESLKRELQEREQLLIKASKAVESLAEAGGS
  EIQRVKEDARKKVQQVEDLLTKRILLLEKDVTAAQAELEKAFAGTETEKALRLRLESKLSEMKKMHEGDL
  AMALVLDEKDRLIEELKLSLKSKEALIQCLKEEKSQMACPDENVSSGELRGLCAAPREEKERETEAAQME
  HQKERNSFEERIQALEEDLREKEREIATEKKNSLKRDKAIQGLTMALKSKEKKVEELNSEIEKLSAAFAK
  AREALQKAQTQEFQGSEDYETALSGKEALSAALRSQNLTKSTENHRLRRSIKKITQELSDLQQERERLEK
  DLEEAHREKSKGDCTIRDLRNEVEKLRNEVNEREKAMENRYKSLLSESNKKLHNQEQVIKHLTESTNQKD
  VLLQKFNEKDLEVIQQNCYLMAAEDLELRSEGLITEKCSSQQPPGSKTIFSKEKKQSSDYEELIQVLKKE
  QDIYTHLVKSLQESDSINNLQAELNKIFALRKQLEQDVLSYQNLRKTLEEQISEIRRREEESFSLYSDQT
  SYLSICLEENNRFQVEHFSQEELKKKVSDLIQLVKELYTDNQHLKKTIFDLSCMGFQGNGFPDRLASTEQ
  TELLASKEDEDTIKIGEDDEINFLSDQHLQQSNEIMKDLSKGGCKNGYLRHTESKISDCDGAHAPGCLEE
  GAFINLLAPLFNEKATLLLESRPDLLKVVRELLLGQLFLTEQEVSGEHLDGKTEKTPKQKGELVHFVQTN
  SFSKPHDELKLSCEAQLVKAGEVPKVGLKDASVQTVATEGDLLRFKHEATREAWEEKPINTALSAEHRPE
  NLHGVPGWQAALLSLPGITNREAKKSRLPILIKPSRSLGNMYRLPATQEVVTQLQSQILELQGELKEFKT
  CNKQLHQKLILAEAVMEGRPTPDKTLLNAQPPVGAAYQDSPGEQKGIKTTSSVWRDKEMDSDQQRSYEID
  SEICPPDDLASLPSCKENPEDVLSPTSVATYLSSKSQPSAKVSVMGTDQSESINTSNETEYLKQKIHDLE
  TELEGYQNFIFQLQKHSQCSEAIITVLCGTEGAQDGLSKPKNGSDGEEMTFSSLHQVRYVKHVKILGPLA
  PEMIDSRVLENLKQQLEEQEYKLQKEQNLNMQLFSEIHNLQNKFRDLSPPRYDSLVQSQARELSLQRQQI
  KDGHGICVISRQHMNTMIKAFEELLQASDVDYCVAEGFQEQLNQCAELLEKLEKLFLNGKSVGVEMNTQN
  ELMERIEEDNLTYQHLLPESPEPSASHALSDYETSEKSFFSRDQKQDNETEKTSVMVNSFSQDLLMEHIQ
  EIRTLRKRLEESIKTNEKLRKQLERQGSEFVQGSTSIFASGSELHSSLTSEIHFLRKQNQALNAMLIKGS
  RDKQKENDKLRESLSRKTVSLEHLQREYASVKEENERLQKEGSEKERHNQQLIQEVRCSGQELSRVQEEV
  KLRQQLLSQNDKLLQSLRVELKAYEKLDEEHRRLRDGDKYPMESDNSFDLFDSSQAVTPKSVSETPPLSG
  NDTDSLSCDSGSSATSTPCVSRLVTGHHLWASKNGRHVLGLIEDYEALLKQISQGQRLLAEMDIQTQEAP
  SSTSQELGTKGPHPAPLSKFVSSVSTAKLTLEEAYRRLKLLWRVSLPEDGQCPLHCEQIGEMKAEVTKLH
  KKLFEQEKKLQNTMKLLQLSKRQEKVIFDQLVVTHKILRKARGNLELRPGGAHPGTCSPSRPGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011649
• ORF Size: 5442 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001011649.1, NM_001011649.2, NP_001011649.1
• RefSeq Size: 5993
• RefSeq ORF: 5445
• Locus ID: 55755
• MW: 206.3 kDa
• Gene Summary: This gene encodes a regulator of CDK5 (cyclin-dependent kinase 5) activity. The protein encoded by this gene is localized to the centrosome and Golgi complex, interacts with CDK5R1 and pericentrin (PCNT), plays a role in centriole engagement and microtubule nucleation, and has been linked to primary microcephaly and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]