ERBIN (NM_001006600) Human Tagged ORF Clone

CAT#: RC224376

ERBB2IP (Myc-DDK-tagged)-Human erbb2 interacting protein (ERBB2IP), transcript variant 7

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ERBIN
• Synonyms: ERBB2IP; HEL-S-78; LAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224376 representing NM_001006600
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACAAAACGAAGTTTGTTTGTGCGGTTGGTACCATGTCGCTGTCTACGAGGGGAAGAGGAGACTG
  TCACTACTCTTGATTATTCTCATTGCAGCTTAGAACAAGTTCCGAAAGAGATTTTTACTTTTGAAAAAAC
  CTTGGAGGAACTCTATTTAGATGCTAATCAGATTGAAGAGCTTCCAAAGCAACTTTTTAACTGTCAGTCT
  TTACACAAACTGAGTTTGCCAGACAATGATTTAACAACGTTACCAGCATCCATTGCAAACCTTATTAATC
  TCAGGGAACTGGATGTCAGCAAGAATGGAATACAGGAGTTTCCAGAAAATATAAAAAATTGTAAAGTTTT
  GACAATTGTGGAGGCCAGTGTAAACCCTATTTCCAAGCTCCCTGATGGATTTTCTCAGCTGTTAAACCTA
  ACCCAGTTGTATCTGAATGATGCTTTTCTTGAGTTCTTGCCAGCAAATTTTGGCAGATTAACTAAACTCC
  AAATATTAGAGCTTAGAGAAAACCAGTTAAAAATGTTGCCTAAAACTATGAATAGACTGACCCAGCTGGA
  AAGACTGGATTTGGGAAGTAACGAATTCACGGAAGTGCCTGAAGTACTTGAGCAACTAAGTGGATTGAAA
  GAGTTTTGGATGGATGCTAATAGACTGACTTTTATTCCAGGGTTTATTGGTAGTTTGAAACAGCTCACAT
  ATTTGGATGTTTCTAAAAATAATATTGAAATGGTTGAAGAAGGAATTTCAACATGTGAAAACCTTCAAGA
  CCTCCTATTATCAAGCAATTCACTTCAGCAGCTTCCTGAGACTATTGGTTCGTTGAAGAATATAACAACG
  CTTAAAATAGATGAAAACCAGTTAATGTATCTGCCAGACTCTATAGGAGGGTTAATATCAGTAGAAGAAC
  TGGATTGTAGTTTCAATGAAGTTGAAGCTTTGCCTTCATCTATTGGGCAGCTTACTAACTTAAGAACTTT
  TGCTGCTGATCATAATTACTTACAGCAGTTGCCCCCAGAGATTGGAAGCTGGAAAAATATAACTGTGCTG
  TTTCTCCATTCCAATAAACTTGAGACACTTCCAGAGGAAATGGGTGATATGCAAAAATTAAAAGTCATTA
  ATTTAAGTGATAATAGATTAAAGAATTTACCCTTTAGCTTTACAAAGCTACAGCAATTGACAGCTATGTG
  GCTCTCAGATAATCAGTCCAAACCCCTGATACCTCTTCAAAAAGAAACTGATTCAGAGACCCAGAAAATG
  GTGCTTACCAACTACATGTTCCCTCAACAGCCAAGGACTGAGGATGTTATGTTTATATCAGATAATGAAA
  GTTTTAACCCTTCATTGTGGGAGGAACAGAGGAAACAGCGGGCTCAAGTTGCATTTGAATGTGATGAAGA
  CAAAGATGAAAGGGAGGCACCTCCCAGGGAGGGAAATTTAAAAAGATATCCAACACCATACCCAGATGAG
  CTTAAGAATATGGTCAAAACTGTTCAAACCATTGTACATAGATTAAAAGATGAAGAGACCAATGAAGACT
  CAGGAAGAGATTTGAAACCACATGAAGATCAACAAGATATAAATAAAGATGTGGGTGTGAAGACCTCAGA
  AAGTACTACTACAGTAAAAAGCAAAGTTGATGAAAGAGAAAAATATATGATAGGAAACTCTGTACAGAAG
  ATCAGTGAACCTGAAGCTGAGATTAGTCCTGGGAGTTTACCAGTGACTGCAAATATGAAAGCCTCTGAGA
  ACTTGAAGCATATTGTTAACCATGATGATGTTTTTGAGGAATCTGAAGAACTTTCTTCTGATGAAGAGAT
  GAAAATGGCGGAGATGCGACCACCATTAATTGAAACCTCTATTAACCAGCCAAAAGTCGTAGCACTTAGT
  AATAACAAAAAAGATGATACAAAGGAAACAGATTCTTTATCAGATGAAGTTACACACAATAGCAATCAGA
  ATAACAGCAATTGTTCTTCTCCATCTCGGATGTCTGATTCAGTTTCTCTTAATACTGATAGTAGTCAAGA
  CACCTCACTCTGCTCTCCAGTGAAACAAACTCATATTGATATTAATTCCAAAATCAGGCAAGAAGATGAA
  AATTTTAACAGCCTTTTACAAAATGGAGATATTTTAAACAGTTCAACAGAGGAAAAGTTCAAAGCTCATG
  ATAAAAAAGATTTTAACTTACCTGAATATGATTTGAATGTTGAAGAGCGATTAGTTCTAATTGAGAAAAG
  TGTTGACTCAACAGCCACAGCTGATGACACTCACAAATTAGATCATATCAATATGAATCTTAATAAACTT
  ATAACTAATGATACATTTCAACCAGAGATCATGGAAAGATCAAAAACACAGGATATTGTGCTTGGAACAA
  GCTTTTTAAGCATTAATTCTAAAGAGGAAACTGAGCACTTGGAAAATGGAAACAAGTATCCTAATTTGGA
  ATCCGTAAATAAGGTAAATGGACATTCTGAGGAAACTTCCCAGTCTCCTAATAGGACTGAACCACATGAC
  AGTGATTGTTCTGTTGACTTAGGTATTTCCAAAAGCACTGAAGATCTCTCCCCTCAGAAAAGTGGTCCAG
  TTGGATCTGTTGTGAAATCTCATAGCATAACTAATATGGAGATTGGAGGGCTAAAAATCTATGATATTCT
  TAGTGATAATGGACCTCAGCAGCCAAGTACAACCGTTAAAATCACATCTGCTGTTGATGGAAAAAATATA
  GTCAGGAGCAAGTCTGCCACACTGTTGTATGATCAACCATTGCAGGTATTTACTGGTTCTTCCTCATCTT
  CTGATTTAATATCAGGAACAAAGGCAATTTTCAAGTTTGATTCAAATCATAATCCCGAAGAGCCAAATAT
  AATAAGAGGCCCCACAAGTGGCCCACAATCTGCACCTCAAATATATGGTCCTCCACAGTATAATATCCAA
  TACAGTAGCAGTGCTGCAGTCAAAGACACTTTGTGGCACTCCAAACAAAATCCCCAAATAGACCATGCCA
  GTTTTCCTCCTCAGCTCCTTCCTAGATCAGAGAGCACAGAAAATCAAAGTTATGCTAAACATTCTGCCAA
  TATGAATTTCTCTAATCATAACAATGTTCGAGCTAATACTGCATACCATTTACATCAGAGACTTGGCCCA
  GCAAGACATGGGGAAATGTGGGCCATCTCACCAAACGACCGACTTATTCCTGCAGTAACTCGAAGTACAA
  TCCAGCGACAAAGTAGTGTGTCCTCCACAGCCTCTGTAAATCTTGGTGATCCAGGCTCTACAAGGCGGGC
  TCAGATTCCTGAAGGAGATTATTTATCATACAGAGAGTTCCACTCAGCGGGAAGAACTCCTCCAATGATG
  CCAGGATCACAGAGACCCCTTTCTGCACGAACATACAGCATAGATGGTCCAAATGCATCAAGACCTCAGA
  GTGCTCGACCCTCTATTAATGAAATACCAGAGAGAACTATGTCAGTTAGTGATTTCAATTATTCACGGAC
  TAGTCCTTCAAAAAGACCAAATGCAAGGGTTGGTTCTGAGCATTCTTTATTAGATCCTCCAGGAAAAAGT
  AAAGTTCCTCGTGACTGGAGAGAACAAGTACTTCGACATATTGAAGCCAAAAAGTTAGAAAAGATTCGAG
  TGAGGGTTGAAAAGGATCCAGAACTTGGATTTAGCATATCAGGTGGTGTCGGGGGTAGAGGAAACCCATT
  CAGACCTGATGATGATGGTATATTTGTAACAAGGGTACAACCTGAAGGACCAGCATCAAAATTACTGCAG
  CCAGGTGATAAAATTATTCAGGCTAATGGCTACAGTTTTATAAATATTGAACATGGACAAGCAGTGTCCT
  TGCTAAAAACTTTCCAGAATACAGTTGAACTCATCATTGTACGAGAAGTTTCCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224376 representing NM_001006600
  Red=Cloning site Green=Tags(s)
  
  MTTKRSLFVRLVPCRCLRGEEETVTTLDYSHCSLEQVPKEIFTFEKTLEELYLDANQIEELPKQLFNCQS
  LHKLSLPDNDLTTLPASIANLINLRELDVSKNGIQEFPENIKNCKVLTIVEASVNPISKLPDGFSQLLNL
  TQLYLNDAFLEFLPANFGRLTKLQILELRENQLKMLPKTMNRLTQLERLDLGSNEFTEVPEVLEQLSGLK
  EFWMDANRLTFIPGFIGSLKQLTYLDVSKNNIEMVEEGISTCENLQDLLLSSNSLQQLPETIGSLKNITT
  LKIDENQLMYLPDSIGGLISVEELDCSFNEVEALPSSIGQLTNLRTFAADHNYLQQLPPEIGSWKNITVL
  FLHSNKLETLPEEMGDMQKLKVINLSDNRLKNLPFSFTKLQQLTAMWLSDNQSKPLIPLQKETDSETQKM
  VLTNYMFPQQPRTEDVMFISDNESFNPSLWEEQRKQRAQVAFECDEDKDEREAPPREGNLKRYPTPYPDE
  LKNMVKTVQTIVHRLKDEETNEDSGRDLKPHEDQQDINKDVGVKTSESTTTVKSKVDEREKYMIGNSVQK
  ISEPEAEISPGSLPVTANMKASENLKHIVNHDDVFEESEELSSDEEMKMAEMRPPLIETSINQPKVVALS
  NNKKDDTKETDSLSDEVTHNSNQNNSNCSSPSRMSDSVSLNTDSSQDTSLCSPVKQTHIDINSKIRQEDE
  NFNSLLQNGDILNSSTEEKFKAHDKKDFNLPEYDLNVEERLVLIEKSVDSTATADDTHKLDHINMNLNKL
  ITNDTFQPEIMERSKTQDIVLGTSFLSINSKEETEHLENGNKYPNLESVNKVNGHSEETSQSPNRTEPHD
  SDCSVDLGISKSTEDLSPQKSGPVGSVVKSHSITNMEIGGLKIYDILSDNGPQQPSTTVKITSAVDGKNI
  VRSKSATLLYDQPLQVFTGSSSSSDLISGTKAIFKFDSNHNPEEPNIIRGPTSGPQSAPQIYGPPQYNIQ
  YSSSAAVKDTLWHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTAYHLHQRLGP
  ARHGEMWAISPNDRLIPAVTRSTIQRQSSVSSTASVNLGDPGSTRRAQIPEGDYLSYREFHSAGRTPPMM
  PGSQRPLSARTYSIDGPNASRPQSARPSINEIPERTMSVSDFNYSRTSPSKRPNARVGSEHSLLDPPGKS
  KVPRDWREQVLRHIEAKKLEKIRVRVEKDPELGFSISGGVGGRGNPFRPDDDGIFVTRVQPEGPASKLLQ
  PGDKIIQANGYSFINIEHGQAVSLLKTFQNTVELIIVREVSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001006600
• ORF Size: 3906 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001006600.1, NM_001006600.2, NP_001006600.1
• RefSeq Size: 6712 bp
• RefSeq ORF: 3909 bp
• Locus ID: 55914
• Cytogenetics: 5q12.3
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 146.1 kDa
• Gene Summary: This gene is a member of the leucine-rich repeat and PDZ domain (LAP) family. The encoded protein contains 17 leucine-rich repeats and one PDZ domain. It binds to the unphosphorylated form of the ERBB2 protein and regulates ERBB2 function and localization. It has also been shown to affect the Ras signaling pathway by disrupting Ras-Raf interaction. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]