LAR (PTPRF) (NM_002840) Human Tagged ORF Clone

CAT#: RC224381

PTPRF (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTPRF
• Synonyms: BNAH2; LAR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224381 representing NM_002840
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCTGAGCCAGCCCCAGGGAGGACGATGGTGCCCCTTGTGCCTGCACTGGTGATGCTTGGTTTGG
  TGGCAGGCGCCCATGGTGACAGCAAACCTGTCTTCATTAAAGTCCCTGAGGACCAGACTGGGCTGTCAGG
  AGGGGTAGCCTCCTTCGTGTGCCAAGCTACAGGAGAACCCAAGCCGCGCATCACATGGATGAAGAAGGGG
  AAGAAAGTCAGCTCCCAGCGCTTCGAGGTCATTGAGTTTGATGATGGGGCAGGGTCAGTGCTTCGGATCC
  AGCCATTGCGGGTGCAGCGAGATGAAGCCATCTATGAGTGTACAGCTACTAACAGCCTGGGTGAGATCAA
  CACTAGTGCCAAGCTCTCAGTGCTCGAAGAGGAACAGCTGCCCCCTGGGTTCCCTTCCATCGACATGGGG
  CCTCAGCTGAAGGTGGTGGAGAAGGCACGCACAGCCACCATGCTATGTGCCGCAGGCGGAAATCCAGACC
  CTGAGATTTCTTGGTTCAAGGACTTCCTTCCTGTAGACCCTGCCACGAGCAACGGCCGCATCAAGCAGCT
  GCGTTCAGGTGCCTTGCAGATAGAGAGCAGTGAGGAATCCGACCAAGGCAAGTACGAGTGTGTGGCGACC
  AACTCGGCAGGCACACGTTACTCAGCCCCTGCGAACCTGTATGTGCGAGTGCGCCGCGTGGCTCCTCGTT
  TCTCCATCCCTCCCAGCAGCCAGGAGGTGATGCCAGGCGGCAGCGTGAACCTGACATGCGTGGCAGTGGG
  TGCACCCATGCCCTACGTGAAGTGGATGATGGGGGCCGAGGAGCTCACCAAGGAGGATGAGATGCCAGTT
  GGCCGCAACGTCCTGGAGCTCAGCAATGTCGTACGCTCTGCCAACTACACCTGTGTGGCCATCTCCTCGC
  TGGGCATGATCGAGGCCACAGCCCAGGTCACAGTGAAAGCTCTTCCAAAGCCTCCGATTGATCTTGTGGT
  GACAGAGACAACTGCCACCAGTGTCACCCTCACCTGGGACTCTGGGAACTCGGAGCCTGTAACCTACTAT
  GGCATCCAGTACCGCGCAGCGGGCACGGAGGGCCCCTTTCAGGAGGTGGATGGTGTGGCCACCACCCGCT
  ACAGCATTGGCGGCCTCAGCCCTTTCTCGGAATATGCCTTCCGCGTGCTGGCGGTGAACAGCATCGGGCG
  AGGGCCGCCCAGCGAGGCAGTGCGGGCACGCACGGGAGAACAGGCGCCCTCCAGCCCACCGCGCCGCGTG
  CAGGCACGCATGCTGAGCGCCAGCACCATGCTGGTGCAGTGGGAGCCTCCCGAGGAGCCCAACGGCCTGG
  TGCGGGGATACCGCGTCTACTATACTCCGGACTCCCGCCGCCCCCCGAACGCCTGGCACAAGCACAACAC
  CGACGCGGGGCTCCTCACGACCGTGGGCAGCCTGCTGCCTGGCATCACCTACAGCCTGCGCGTGCTTGCC
  TTCACCGCCGTGGGCGATGGCCCTCCCAGCCCCACCATCCAGGTCAAGACGCAGCAGGGAGTGCCTGCCC
  AGCCCGCGGACTTCCAGGCCGAGGTGGAGTCGGACACCAGGATCCAGCTCTCGTGGCTGCTGCCCCCTCA
  GGAGCGGATCATCATGTATGAACTGGTGTACTGGGCGGCAGAGGACGAAGACCAACAGCACAAGGTGACC
  TTCGACCCAACCTCCTCCTACACACTAGAGGACCTGAAGCCTGACACACTCTACCGCTTCCAGCTGGCTG
  CACGCTCGGATATGGGGGTGGGCGTCTTCACCCCCACCATTGAGGCCCGCACAGCCCAGTCCACCCCCTC
  CGCCCCTCCCCAGAAGGTGATGTGTGTGAGCATGGGCTCCACCACGGTCCGGGTAAGTTGGGTCCCGCCG
  CCTGCCGACAGCCGCAACGGCGTTATCACCCAGTACTCCGTGGCCTACGAGGCGGTGGACGGCGAGGACC
  GCGGGCGGCATGTGGTGGATGGCATCAGCCGTGAGCACTCCAGCTGGGACCTGGTGGGCCTGGAGAAGTG
  GACGGAGTACCGGGTGTGGGTGCGGGCACACACAGACGTGGGCCCCGGCCCCGAGAGCAGCCCGGTGCTG
  GTGCGCACCGATGAGGACGTGCCCAGCGGGCCTCCGCGGAAGGTGGAGGTGGAGCCACTGAACTCCACTG
  CTGTGCATGTCTACTGGAAGCTGCCTGTCCCCAGCAAGCAGCATGGCCAGATCCGCGGCTACCAGGTCAC
  CTACGTGCGGCTGGAGAATGGCGAGCCCCGTGGACTCCCCATCATCCAAGACGTCATGCTAGCCGAGGCC
  CAGTGGCGGCCAGAGGAGTCCGAGGACTATGAAACCACTATCAGCGGCCTGACCCCGGAGACCACCTACT
  CCGTTACTGTTGCTGCCTATACCACCAAGGGGGATGGTGCCCGCAGCAAGCCCAAAATTGTCACTACAAC
  AGGTGCAGTCCCAGGCCGGCCCACCATGATGATCAGCACCACGGCCATGAACACTGCGCTGCTCCAGTGG
  CACCCACCCAAGGAACTGCCTGGCGAGCTGCTGGGCTACCGGCTGCAGTACTGCCGGGCCGACGAGGCGC
  GGCCCAACACCATAGATTTCGGCAAGGATGACCAGCACTTCACAGTCACCGGCCTGCACAAGGGGACCAC
  CTACATCTTCCGGCTTGCTGCCAAGAACCGGGCTGGCTTGGGTGAGGAGTTCGAGAAGGAGATCAGGACC
  CCCGAGGACCTGCCCAGCGGCTTCCCCCAAAACCTGCATGTGACAGGACTGACCACGTCTACCACAGAAC
  TGGCCTGGGACCCGCCAGTGCTGGCGGAGAGGAACGGGCGCATCATCAGCTACACCGTGGTGTTCCGAGA
  CATCAACAGCCAACAGGAGCTGCAGAACATCACGACAGACACCCGCTTTACCCTTACTGGCCTCAAGCCA
  GACACCACTTACGACATCAAGGTCCGCGCATGGACCAGCAAAGGCTCTGGCCCACTCAGCCCCAGCATCC
  AGTCCCGGACCATGCCGGTGGAGCAAGTGTTTGCCAAGAACTTCCGGGTGGCGGCTGCAATGAAGACGTC
  TGTGCTGCTCAGCTGGGAGGTTCCCGACTCCTATAAGTCAGCTGTGCCCTTTAAGATTCTGTACAATGGG
  CAGAGTGTGGAGGTGGACGGGCACTCGATGCGGAAGCTGATCGCAGACCTGCAGCCCAACACAGAGTACT
  CGTTTGTGCTGATGAACCGTGGCAGCAGCGCAGGGGGCCTGCAGCACCTGGTGTCCATCCGCACAGCCCC
  CGACCTCCTGCCTCACAAGCCGCTGCCTGCCTCTGCCTACATAGAGGACGGCCGCTTCGATCTCTCCATG
  CCCCATGTGCAAGACCCCTCGCTTGTCAGGTGGTTCTACATTGTTGTGGTGCCCATTGACCGTGTGGGCG
  GGAGCATGCTGACGCCAAGGTGGAGCACACCCGAGGAACTGGAGCTGGACGAGCTTCTAGAAGCCATCGA
  GCAAGGCGGAGAGGAGCAGCGGCGGCGGCGGCGGCAGGCAGAACGTCTGAAGCCATATGTGGCTGCTCAA
  CTGGATGTGCTCCCGGAGACCTTTACCTTGGGGGACAAGAAGAACTACCGGGGCTTCTACAACCGGCCCC
  TGTCTCCGGACTTGAGCTACCAGTGCTTTGTGCTTGCCTCCTTGAAGGAACCCATGGACCAGAAGCGCTA
  TGCCTCCAGCCCCTACTCGGATGAGATCGTGGTCCAGGTGACACCAGCCCAGCAGCAGGAGGAGCCGGAG
  ATGCTGTGGGTGACGGGTCCCGTGCTGGCAGTCATCCTCATCATCCTCATTGTCATCGCCATCCTCTTGT
  TCAAAAGGAAAAGGACCCACTCTCCGTCCTCTAAGGATGAGCAGTCGATCGGACTGAAGGACTCCTTGCT
  GGCCCACTCCTCTGACCCTGTGGAGATGCGGAGGCTCAACTACCAGACCCCAGGTATGCGAGACCACCCA
  CCCATCCCCATCACCGACCTGGCGGACAACATCGAGCGCCTCAAAGCCAACGATGGCCTCAAGTTCTCCC
  AGGAGTATGAGTCCATCGACCCTGGACAGCAGTTCACGTGGGAGAATTCAAACCTGGAGGTGAACAAGCC
  CAAGAACCGCTATGCGAATGTCATCGCCTACGACCACTCTCGAGTCATCCTTACCTCTATCGATGGCGTC
  CCCGGGAGTGACTACATCAATGCCAACTACATCGATGGCTACCGCAAGCAGAATGCCTACATCGCCACGC
  AGGGCCCCCTGCCCGAGACCATGGGTGATTTCTGGAGGATGGTGTGGGAACAGCGCACGGCCACTGTGGT
  CATGATGACACGGCTGGAGGAGAAGTCCCGGGTAAAATGTGATCAGTACTGGCCAGCCCGTGGCACCGAG
  ACCTGTGGCCTTATTCAGGTGACCCTGTTGGACACAGTGGAGCTGGCCACATACACTGTGCGCACCTTCG
  CACTCCACAAGAGTGGCTCCAGTGAGAAGCGCGAGCTGCGTCAGTTTCAGTTCATGGCCTGGCCAGACCA
  TGGAGTTCCTGAGTACCCAACTCCCATCCTGGCCTTCCTACGACGGGTCAAGGCCTGCAACCCCCTAGAC
  GCAGGGCCCATGGTGGTGCACTGCAGCGCGGGCGTGGGCCGCACCGGCTGCTTCATCGTGATTGATGCCA
  TGTTGGAGCGGATGAAGCACGAGAAGACGGTGGACATCTATGGCCACGTGACCTGCATGCGATCACAGAG
  GAACTACATGGTGCAGACGGAGGACCAGTACGTGTTCATCCATGAGGCGCTGCTGGAGGCTGCCACGTGC
  GGCCACACAGAGGTGCCTGCCCGCAACCTGTATGCCCACATCCAGAAGCTGGGCCAAGTGCCTCCAGGGG
  AGAGTGTGACCGCCATGGAGCTCGAGTTCAAGTTGCTGGCCAGCTCCAAGGCCCACACGTCCCGCTTCAT
  CAGCGCCAACCTGCCCTGCAACAAGTTCAAGAACCGGCTGGTGAACATCATGCCCTACGAATTGACCCGT
  GTGTGTCTGCAGCCCATCCGTGGTGTGGAGGGCTCTGACTACATCAATGCCAGCTTCCTGGATGGTTATA
  GACAGCAGAAGGCCTACATAGCTACACAGGGGCCTCTGGCAGAGAGCACCGAGGACTTCTGGCGCATGCT
  ATGGGAGCACAATTCCACCATCATCGTCATGCTGACCAAGCTTCGGGAGATGGGCAGGGAGAAATGCCAC
  CAGTACTGGCCAGCAGAGCGCTCTGCTCGCTACCAGTACTTTGTTGTTGACCCGATGGCTGAGTACAACA
  TGCCCCAGTATATCCTGCGTGAGTTCAAGGTCACGGATGCCCGGGATGGGCAGTCAAGGACAATCCGGCA
  GTTCCAGTTCACAGACTGGCCAGAGCAGGGCGTGCCCAAGACAGGCGAGGGATTCATTGACTTCATCGGG
  CAGGTGCATAAGACCAAGGAGCAGTTTGGACAGGATGGGCCTATCACGGTGCACTGCAGTGCTGGCGTGG
  GCCGCACCGGGGTGTTCATCACTCTGAGCATCGTCCTGGAGCGCATGCGCTACGAGGGCGTGGTCGACAT
  GTTTCAGACCGTGAAGACCCTGCGTACACAGCGTCCTGCCATGGTGCAGACAGAGGACCAGTATCAGCTG
  TGCTACCGTGCGGCCCTGGAGTACCTCGGCAGCTTTGACCACTATGCAACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224381 representing NM_002840
  Red=Cloning site Green=Tags(s)
  
  MAPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKG
  KKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMG
  PQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVAT
  NSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPV
  GRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVTYY
  GIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRV
  QARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPPNAWHKHNTDAGLLTTVGSLLPGITYSLRVLA
  FTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVESDTRIQLSWLLPPQERIIMYELVYWAAEDEDQQHKVT
  FDPTSSYTLEDLKPDTLYRFQLAARSDMGVGVFTPTIEARTAQSTPSAPPQKVMCVSMGSTTVRVSWVPP
  PADSRNGVITQYSVAYEAVDGEDRGRHVVDGISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVL
  VRTDEDVPSGPPRKVEVEPLNSTAVHVYWKLPVPSKQHGQIRGYQVTYVRLENGEPRGLPIIQDVMLAEA
  QWRPEESEDYETTISGLTPETTYSVTVAAYTTKGDGARSKPKIVTTTGAVPGRPTMMISTTAMNTALLQW
  HPPKELPGELLGYRLQYCRADEARPNTIDFGKDDQHFTVTGLHKGTTYIFRLAAKNRAGLGEEFEKEIRT
  PEDLPSGFPQNLHVTGLTTSTTELAWDPPVLAERNGRIISYTVVFRDINSQQELQNITTDTRFTLTGLKP
  DTTYDIKVRAWTSKGSGPLSPSIQSRTMPVEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNG
  QSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFDLSM
  PHVQDPSLVRWFYIVVVPIDRVGGSMLTPRWSTPEELELDELLEAIEQGGEEQRRRRRQAERLKPYVAAQ
  LDVLPETFTLGDKKNYRGFYNRPLSPDLSYQCFVLASLKEPMDQKRYASSPYSDEIVVQVTPAQQQEEPE
  MLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHP
  PIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGV
  PGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTE
  TCGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLD
  AGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATC
  GHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTR
  VCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTKLREMGREKCH
  QYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIG
  QVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQL
  CYRAALEYLGSFDHYAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002840
• ORF Size: 5721 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002840.3, NM_002840.4, NP_002831.2
• RefSeq Size: 7733 bp
• RefSeq ORF: 5724 bp
• Locus ID: 5792
• Cytogenetics: 1p34.2
• Domains: Y_phosphatase, ig, PTPc_motif, IGc2, IG, FN3
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Adherens junction, Cell adhesion molecules (CAMs), Insulin signaling pathway
• MW: 212.88 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]'