ARPC4 (NM_005718) Human Tagged ORF Clone

CAT#: RC224478

ARPC4 (Myc-DDK-tagged)-Human actin related protein 2/3 complex, subunit 4, 20kDa (ARPC4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401744) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224478 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARPC4
• Synonyms: ARC20; P20-ARC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224478 representing NM_005718
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGCCACTCTCCGCCCCTACCTGAGTGCCGTGCGGGCCACATTGCAGGCTGCCCTCTGCCTGGAGA
  ACTTCTCCTCCCAGGTTGTGGAACGACACAACAAGCCGGAAGTGGAAGTCAGGAGTAGCAAAGAGCTCCT
  GTTACAACCTGTGACCATCAGCAGGAATGAGAAGGAAAAGGTTCTGATTGAGGGCTCCATCAACTCTGTC
  CGGGTCAGCATTGCTGTGAAACAGGCTGATGAGATCGAGAAGATTTTGTGCCACAAGTTCATGCGCTTCA
  TGATGATGCGAGCAGAGAACTTCTTTATCCTTCGAAGGAAGCCTGTGGAGGGGTATGATATCAGCTTTCT
  GATCACCAACTTCCACACAGAGCAGATGTACAAACACAAGTTGGTGGACTTTGTGATCCACTTCATGGAG
  GAGATTGACAAGGAGATCAGTGAGATGAAGCTGTCAGTCAATGCCCGTGCCCGCATTGTGGCTGAAGAGT
  TCCTTAAGAATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224478 representing NM_005718
  Red=Cloning site Green=Tags(s)
  
  MTATLRPYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVTISRNEKEKVLIEGSINSV
  RVSIAVKQADEIEKILCHKFMRFMMMRAENFFILRRKPVEGYDISFLITNFHTEQMYKHKLVDFVIHFME
  EIDKEISEMKLSVNARARIVAEEFLKNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005718
• ORF Size: 504 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005718.1, NM_005718.2, NM_005718.3, NM_005718.4, NP_005709.1
• RefSeq Size: 1976 bp
• RefSeq ORF: 507 bp
• Locus ID: 10093
• Protein Pathways: Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• MW: 19.5 kDa
• Gene Summary: This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit, which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring read-through transcription exists between this gene and the downstream tubulin tyrosine ligase-like family, member 3 (TTLL3), which results in the production of a fusion protein. [provided by RefSeq, Nov 2010]