WFDC8 (NM_130896) Human Tagged ORF Clone
CAT#: RC224509
- TrueORF®
WFDC8 (Myc-DDK-tagged)-Human WAP four-disulfide core domain 8 (WFDC8), transcript variant a
"NM_130896" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | WFDC8 |
Synonyms | C20orf170; dJ461P17.1; HEL-S-292; WAP8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224509 representing NM_130896
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGACTGTCCGAACTGAAGGAGGGCACTTTCCTCTCCATAGCCCCACCTTCTCCTGGAGGAATGTAG CTTTCCTGCTGCTTCTCTCCCTTGCTTTGGAGTGGACTTCTGCAATGCTGACCAAGAAGATCAAACACAA ACCAGGGTTATGTCCCAAAGAGAGGCTCACCTGTACCACTGAACTTCCGGACTCATGTAACACAGATTTT GACTGCAAGGAATACCAGAAGTGCTGCTTTTTTGCCTGTCAGAAGAAGTGCATGGATCCCTTTCAAGAAC CCTGCATGCTACCTGTGAGGCATGGAAACTGTAATCATGAGGCACAGCGCTGGCATTTTGACTTTAAAAA TTACCGCTGCACACCCTTCAAATACAGGGGCTGCGAAGGGAATGCCAACAACTTCTTAAGTGAGGATGCC TGCAGAACGGCCTGCATGTTAATTGTTAAGGATGGACAATGCCCACTCTTCCCTTTCACTGAACGTAAGG AGTGTCCACCTTCATGTCACAGTGACATCGATTGTCCCCAGACAGACAAATGTTGTGAATCCAGGTGTGG CTTTGTTTGTGCCAGGGCCTGGACAGTCAAAAAAGGTTTCTGCCCACGCAAGCCCTTGCTATGTACCAAG ATTGATAAACCCAAGTGCCTGCAGGATGAGGAGTGCCCATTGGTGGAAAAGTGCTGCTCACATTGTGGAC TGAAATGTATGGACCCCAGACGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224509 representing NM_130896
Red=Cloning site Green=Tags(s) MWTVRTEGGHFPLHSPTFSWRNVAFLLLLSLALEWTSAMLTKKIKHKPGLCPKERLTCTTELPDSCNTDF DCKEYQKCCFFACQKKCMDPFQEPCMLPVRHGNCNHEAQRWHFDFKNYRCTPFKYRGCEGNANNFLSEDA CRTACMLIVKDGQCPLFPFTERKECPPSCHSDIDCPQTDKCCESRCGFVCARAWTVKKGFCPRKPLLCTK IDKPKCLQDEECPLVEKCCSHCGLKCMDPRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_130896 |
ORF Size | 723 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_130896.1, NM_130896.2, NP_570966.1 |
RefSeq Size | 1069 bp |
RefSeq ORF | 726 bp |
Locus ID | 90199 |
Protein Families | Secreted Protein |
MW | 23.4 kDa |
Gene Summary | This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. The encoded protein contains a Kunitz-inhibitor domain, in addition to three WFDC domains. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the telomeric cluster. Two alternatively spliced transcript variants have been found for this gene, and they encode the same protein. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC305924 | WFDC8 (untagged)-Human WAP four-disulfide core domain 8 (WFDC8), transcript variant a |
USD 540.00 |
|
RG224509 | WFDC8 (GFP-tagged) - Human WAP four-disulfide core domain 8 (WFDC8), transcript variant a |
USD 460.00 |
|
RC224509L1 | Lenti ORF clone of Human WAP four-disulfide core domain 8 (WFDC8), transcript variant a, Myc-DDK-tagged |
USD 620.00 |
|
RC224509L2 | Lenti ORF clone of Human WAP four-disulfide core domain 8 (WFDC8), transcript variant a, mGFP tagged |
USD 620.00 |
|
RC224509L3 | Lenti ORF clone of Human WAP four-disulfide core domain 8 (WFDC8), transcript variant a, Myc-DDK-tagged |
USD 620.00 |
|
RC224509L4 | Lenti ORF clone of Human WAP four-disulfide core domain 8 (WFDC8), transcript variant a, mGFP tagged |
USD 620.00 |
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