KCNMB3 (NM_014407) Human Tagged ORF Clone
CAT#: RC224647
KCNMB3 (Myc-DDK-tagged)-Human potassium large conductance calcium-activated channel, subfamily M beta member 3 (KCNMB3), transcript variant 4
"NM_014407" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | KCNMB3 |
Synonyms | BKBETA3; HBETA3; K(VCA)BETA-3; KCNMB2; KCNMBL; SLO-BETA-3; SLOBETA3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224647 representing NM_014407
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTTTCACCAAGCTCTGAACTGGGATTTCATTTTGTTGCATTCATCCTGCTCACGAGACACAGGA CAGCCTTTCCTGCCTCAGGGAAGAAGAGAGAGACAGACTACAGTGATGGAGACCCACTAGATGTGCACAA GAGGCTGCCATCCAGTGCTGGAGAGGACCGAGCCGTGATGCTGGGGTTTGCCATGATGGGCTTCTCAGTC CTAATGTTCTTCTTGCTCGGAACAACCATTCTAAAGCCTTTTATGCTCAGCATTCAGAGAGAAGAATCGA CCTGCACTGCCATCCACACAGATATCATGGACGACTGGCTGGACTGTGCCTTCACCTGTGGTGTGCACTG CCACGGTCAGGGGAAGTACCCGTGTCTTCAGGTGTTTGTGAACCTCAGCCATCCAGGTCAGAAAGCTCTC CTACATTATAATGAAGAGGCTGTCCAGATAAATCCCAAGTGCTTTTACACACCTAAGTGCCACCAAGATA GAAATGATTTGCTCAACAGTGCTCTGGACATAAAAGAATTCTTCGATCACAAAAATGGAACCCCCTTTTC ATGCTTCTACAGTCCAGCCAGCCAATCTGAAGATGTCATTCTTATAAAAAAGTATGACCAAATGGCTATC TTCCACTGTTTATTTTGGCCTTCACTGACTCTGCTAGGTGGTGCCCTGATTGTTGGCATGGTGAGATTAA CACAACACCTGTCCTTACTGTGTGAAAAATATAGCACTGTAGTCAGAGATGAGGTAGGTGGAAAAGTACC TTATATAGAACAGCATCAGTTCAAACTGTGCATTATGAGGAGGAGCAAAGGAAGAGCAGAGAAATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224647 representing NM_014407
Red=Cloning site Green=Tags(s) MDFSPSSELGFHFVAFILLTRHRTAFPASGKKRETDYSDGDPLDVHKRLPSSAGEDRAVMLGFAMMGFSV LMFFLLGTTILKPFMLSIQREESTCTAIHTDIMDDWLDCAFTCGVHCHGQGKYPCLQVFVNLSHPGQKAL LHYNEEAVQINPKCFYTPKCHQDRNDLLNSALDIKEFFDHKNGTPFSCFYSPASQSEDVILIKKYDQMAI FHCLFWPSLTLLGGALIVGMVRLTQHLSLLCEKYSTVVRDEVGGKVPYIEQHQFKLCIMRRSKGRAEKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_014407 |
ORF Size | 837 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014407.2, NM_014407.3, NP_055222.3 |
RefSeq Size | 1492 bp |
RefSeq ORF | 840 bp |
Locus ID | 27094 |
Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
Protein Pathways | Vascular smooth muscle contraction |
MW | 31.4 kDa |
Gene Summary | MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which may partially inactivate or slightly decrease the activation time of MaxiK alpha subunit currents. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 22. [provided by RefSeq, Jul 2009] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC123923 | KCNMB3 (untagged)-Human potassium large conductance calcium-activated channel, subfamily M beta member 3 (KCNMB3), transcript variant 4 |
USD 660.00 |
|
RG224647 | KCNMB3 (GFP-tagged) - Human potassium large conductance calcium-activated channel, subfamily M beta member 3 (KCNMB3), transcript variant 4 |
USD 460.00 |
|
RC224647L3 | Lenti ORF clone of Human potassium large conductance calcium-activated channel, subfamily M beta member 3 (KCNMB3), transcript variant 4, Myc-DDK-tagged |
USD 620.00 |
|
RC224647L4 | Lenti ORF clone of Human potassium large conductance calcium-activated channel, subfamily M beta member 3 (KCNMB3), transcript variant 4, mGFP tagged |
USD 620.00 |
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