EN1 (NM_001426) Human Tagged ORF Clone
CAT#: RC224659L4
- LentiORF®
Lenti ORF clone of Human engrailed homeobox 1 (EN1), mGFP tagged
"NM_001426" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | EN1 |
Synonyms | engrailed homeobox 1; engrailed homolog 1; OTTHUMP00000162091 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC224659).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001426 |
ORF Size | 1176 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001426.2, NP_001417.2 |
RefSeq Size | 2943 bp |
RefSeq ORF | 1179 bp |
Locus ID | 2019 |
Cytogenetics | 2q14.2 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS |
MW | 39.9 kDa |
Gene Summary | 'Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC303016 | EN1 (untagged)-Human engrailed homeobox 1 (EN1) |
USD 670.00 |
|
RC224659 | EN1 (Myc-DDK-tagged)-Human engrailed homeobox 1 (EN1) |
USD 420.00 |
|
RG224659 | EN1 (GFP-tagged) - Human engrailed homeobox 1 (EN1) |
USD 460.00 |
|
RC224659L3 | Lenti ORF clone of Human engrailed homeobox 1 (EN1), Myc-DDK-tagged |
USD 620.00 |
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