WIPI2 (NM_001033519) Human Tagged ORF Clone

CAT#: RC224661

WIPI2 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY422378) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224661 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WIPI2
• Synonyms: ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224661 representing NM_001033519
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTGGCGAGCCAGAGCGGGGAGGCCGGCGCCGGCCAGCTGCTCTTCGCCAACTTCAACCAGGACA
  ACACGTCCCTAGCTGTTGGTAGTAAGTCCGGTTATAAATTTTTCTCCCTTTCTTCTGTGGATAAGCTGGA
  ACAGATCTATGAATGCACCGATACGGAAGATGTGTGCATTGTAGAGAGATTGTTCTCCAGCAGCCTAGTG
  GCCATCGTCAGCCTTAAAGCACCAAGGAAGCTAAAGGTTTGCCACTTTAAGAAGGGAACTGAGATCTGCA
  ACTACAGCTACTCCAACACGATTCTGGCTGTGAAGCTCAACAGGCAGAGGCTGATAGTATGCCTGGAGGA
  GTCCCTGTACATCCACAACATTCGGGACATGAAGGTGCTGCATACGATCAGGGAGACGCCTCCAAACCCT
  GCAGGCCTGTGTGCGCTGTCAATCAACAACGACAACTGCTACTTGGCGTACCCAGGGAGCGCGACCATCG
  GAGAGGTGCAGGTCTTCGATACCATTAATTTGAGAGCTGCAAACATGATTCCGGCTCACGACAGTCCTTT
  AGCGGCACTGGCCTTTGACGCAAGTGGAACTAAACTTGCCACGGCTTCGGAGAAGGGGACCGTGATTAGG
  GTATTTTCCATTCCAGAAGGACAAAAACTCTTTGAGTTTCGGAGAGGAGTAAAGAGGTGCGTGAGCATCT
  GCTCCCTGGCCTTCAGCATGGACGGCATGTTCCTCTCCGCCTCCAGCAACACTGAGACCGTGCACATCTT
  CAAACTCGAGACTGTGAAAGAAAAACCCCCAGAGGAGCCCACCACCTGGACCGGGTACTTCGGGAAAGTG
  CTCATGGCCTCCACCAGCTACCTGCCTTCCCAAGTGACAGAAATGTTCAACCAGGGCAGAGCCTTCGCCA
  CGGTCCGCCTGCCATTCTGCGGCCACAAAAACATCTGCTCGCTAGCCACAATTCAGAAGATCCCGCGGTT
  GTTGGTGGGTGCCGCCGACGGGTACCTGTACATGTACAACCTGGACCCCCAGGAGGGCGGCGAGTGTGCC
  CTGATGAAGCAGCACCGGCTGGACGGCAGTCTGGAAACGACCAATGAGATCTTGGACTCTGCCTCTCACG
  ACTGCCCCTTAGTCACTCAGACATACGGCGCAGCTGCAGGAAAAGCCTACACAGACGACCTGGGTGCTGT
  GGGTGGCGCCTGCCTGGAGGACGAGGCCAGCGCCCTGCGCCTGGATGAGGACAGCGAGCACCCGCCCATG
  ATTCTTCGGACTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224661 representing NM_001033519
  Red=Cloning site Green=Tags(s)
  
  MNLASQSGEAGAGQLLFANFNQDNTSLAVGSKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLV
  AIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNP
  AGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIR
  VFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLETVKEKPPEEPTTWTGYFGKV
  LMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGAADGYLYMYNLDPQEGGECA
  LMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKAYTDDLGAVGGACLEDEASALRLDEDSEHPPM
  ILRTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033519
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001033519.1, NP_001028691.1
• RefSeq Size: 4402
• RefSeq ORF: 1278
• Locus ID: 26100
• MW: 46.2 kDa
• Gene Summary: WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]). [supplied by OMIM, Mar 2008]