RGS3 (NM_144488) Human Tagged ORF Clone

CAT#: RC224671

RGS3 (Myc-DDK-tagged)-Human regulator of G-protein signaling 3 (RGS3), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGS3
• Synonyms: C2PA; RGP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224671 representing NM_144488
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGTAATCCCAGCACTTTGGGAGGTTGAGATGGGCAGATCGCAAGGTCAGGAGATCGAGACCATCC
  TGGCTAACAGGTCACATTCAGACAGCACACCTTTGCCCAATTTTCTTTCTGGATCTCACCGTCCTGAGTG
  TTGTACCTGCAGGTTGCTCACAGCCTCTGGAGCCCAAGATAGTCTCCCCTTTGGGAGGAGGCTCTACAGT
  GGTCCCTGGCGAAGTTGTGAAGAGGTCTGCCACGTCTCTGTGCTCAGTGTCCTCTCTACATCCTGTGGCT
  TGAGCCTGAGCTTGCCCATATTCCCTGGCTGGATGGAGTGGCTAAGCCCTGATATCGCTCTGCCCAGAAG
  AGATGAGTGGACTCAAACTTCTCCAGCCAGGAAGAGGATCACGCATGCCAAAGTCCAGGGTGCAGGTCAG
  CTGAGGCTGTCCATTGATGCCCAGGACCGGGTTCTGCTGCTTCACATTATAGAAGGTAAAGGCCTGATCA
  GCAAACAGCCTGGCACCTGTGATCCGTATGTGAAGATTTCTTTGATCCCTGAAGATAGTAGACTACGCCA
  CCAGAAGACGCAGACCGTTCCAGACTGCAGAGACCCGGCTTTCCACGAGCACTTCTTCTTTCCTGTCCAA
  GAGGAGGATGATCAGAAGCGTCTCTTGGTTACTGTGTGGAACAGGGCCAGCCAGTCCAGACAGAGTGGAC
  TCATTGGCTGCATGAGCTTTGGGGTGAAGTCTCTCCTGACTCCAGACAAGGAGATCAGTGGTTGGTACTA
  CCTCCTAGGGGAGCACCTGGGCCGGACCAAGCACTTGAAGGTGGCCAGGCGGCGACTGCGGCCGCTGAGA
  GACCCGCTGCTGAGAATGCCAGGAGGTGGGGACACTGAGAATGGGAAGAAACTAAAGATCACCATCCCGA
  GGGGAAAGGACGGCTTTGGCTTCACCATCTGCTGCGACTCTCCAGTTCGAGTCCAGGCCGTGGATTCCGG
  GGGTCCGGCGGAACGGGCAGGGCTGCAGCAGCTGGACACGGTGCTGCAGCTGAATGAGAGGCCTGTGGAG
  CACTGGAAATGTGTGGAGCTGGCCCACGAGATCCGGAGCTGCCCCAGTGAGATCATCCTACTCGTGTGGC
  GCATGGTCCCCCAGGTCAAGCCAGGACCAGATGGCGGGGTCCTGCGGCGGGCCTCCTGCAAGTCGACACA
  TGACCTCCAGTCACCCCCCAACAAACGGGAGAAGAACTGCACCCATGGGGTCCAGGCACGGCCTGAGCAG
  CGCCACAGCTGCCACCTGGTATGTGACAGCTCTGATGGGCTGCTGCTCGGCGGCTGGGAGCGCTACACCG
  AGGTGGCCAAGCGCGGGGGCCAGCACACCCTGCCTGCACTGTCCCGTGCCACTGCCCCCACCGACCCCAA
  CTACATCATCCTGGCCCCGCTGAATCCTGGGAGCCAGCTGCTCCGGCCTGTGTACCAGGAGGATACCATC
  CCCGAAGAATCAGGGAGTCCCAGTAAAGGGAAGTCCTACACAGGCCTGGGGAAGAAGTCCCGGCTGATGA
  AGACAGTGCAGACCATGAAGGGCCACGGGAACTACCAAAACTGCCCGGTTGTGAGGCCGCATGCCACGCA
  CTCAAGCTATGGCACCTACGTCACCCTGGCCCCCAAAGTCCTGGTGTTCCCTGTCTTTGTTCAGCCTCTA
  GATCTCTGTAATCCTGCCCGGACCCTCCTGCTGTCAGAGGAGCTGCTGCTGTATGAAGGGAGGAACAAGG
  CTGCCGAGGTGACACTGTTTGCCTATTCGGACCTGCTGCTCTTCACCAAGGAGGACGAGCCTGGCCGCTG
  CGACGTCCTGAGGAACCCCCTCTACCTCCAGAGTGTGAAGCTGCAGGAAGGTTCTTCAGAAGACCTGAAA
  TTCTGCGTGCTCTATCTAGCAGAGAAGGCAGAGTGCTTATTCACTTTGGAAGCGCACTCGCAGGAGCAGA
  AGAAGAGAGTGTGCTGGTGCCTGTCGGAGAACATCGCCAAGCAGCAACAGCTGGCAGCATCACCCCCGGA
  CAGCAAGATGTTTGAGACGGAGGCAGATGAGAAGAGGGAGATGGCCTTGGAGGAAGGGAAGGGGCCTGGT
  GCCGAGGATTCCCCACCCAGCAAGGAGCCCTCTCCTGGCCAGGAGCTTCCTCCAGGACAAGACCTTCCAC
  CCAACAAGGACTCCCCTTCTGGGCAGGAACCCGCTCCCAGCCAAGAACCACTGTCCAGCAAAGACTCAGC
  TACCTCTGAAGGATCCCCTCCAGGCCCAGATGCTCCGCCCAGCAAGGATGTGCCACCATGCCAGGAACCC
  CCTCCAGCCCAAGACCTCTCACCCTGCCAGGACCTACCTGCTGGTCAAGAACCCCTGCCTCACCAGGACC
  CTCTACTCACCAAAGACCTCCCTGCCATCCAGGAATCCCCCACCCGGGACCTTCCACCCTGTCAAGATCT
  GCCTCCTAGCCAGGTCTCCCTGCCAGCCAAGGCCCTTACTGAGGACACCATGAGCTCCGGGGACCTACTA
  GCAGCTACTGGGGACCCACCTGCGGCCCCCAGGCCAGCCTTCGTGATCCCTGAGGTCCGGCTGGATAGCA
  CCTACAGCCAGAAGGCAGGGGCAGAGCAGGGCTGCTCGGGAGATGAGGAGGATGCAGAAGAGGCCGAGGA
  GGTGGAGGAGGGGGAGGAAGGGGAGGAGGACGAGGATGAGGACACCAGCGATGACAACTACGGAGAGCGC
  AGTGAGGCCAAGCGCAGCAGCATGATCGAGACGGGCCAGGGGGCTGAGGGTGGCCTCTCACTGCGTGTGC
  AGAACTCGCTGCGGCGCCGGACGCACAGCGAGGGCAGCCTGCTGCAGGAGCCCCGAGGGCCCTGCTTTGC
  CTCCGACACCACCTTGCACTGCTCAGACGGTGAGGGCGCCGCCTCCACCTGGGGCATGCCTTCGCCCAGC
  ACCCTCAAGAAAGAGCTGGGCCGCAATGGTGGCTCCATGCACCACCTTTCCCTCTTCTTCACAGGACACA
  GGAAGATGAGCGGGGCTGACACCGTTGGGGATGATGACGAAGCCTCCCGGAAGAGAAAGAGCAAAAACCT
  AGCCAAGGACATGAAGAACAAGCTGGGGATCTTCAGACGGCGGAATGAGTCCCCTGGAGCCCCTCCCGCG
  GGCAAGGCAGACAAAATGATGAAGTCATTCAAGCCCACCTCAGAGGAAGCCCTCAAGTGGGGCGAGTCCT
  TGGAGAAGCTGCTGGTTCACAAATACGGGTTAGCAGTGTTCCAAGCCTTCCTTCGCACTGAGTTCAGTGA
  GGAGAATCTGGAGTTCTGGTTGGCTTGTGAGGACTTCAAGAAGGTCAAGTCACAGTCCAAGATGGCATCC
  AAGGCCAAGAAGATCTTTGCTGAATACATCGCGATCCAGGCATGCAAGGAGGTCAACCTGGACTCCTACA
  CGCGGGAGCACACCAAGGACAACCTGCAGAGCGTCACGCGGGGCTGCTTCGACCTGGCACAGAAGCGCAT
  CTTCGGGCTCATGGAAAAGGACTCGTACCCTCGCTTTCTCCGTTCTGACCTCTACCTGGACCTTATTAAC
  CAGAAGAAGATGAGTCCCCCGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224671 representing NM_144488
  Red=Cloning site Green=Tags(s)
  
  MPVIPALWEVEMGRSQGQEIETILANRSHSDSTPLPNFLSGSHRPECCTCRLLTASGAQDSLPFGRRLYS
  GPWRSCEEVCHVSVLSVLSTSCGLSLSLPIFPGWMEWLSPDIALPRRDEWTQTSPARKRITHAKVQGAGQ
  LRLSIDAQDRVLLLHIIEGKGLISKQPGTCDPYVKISLIPEDSRLRHQKTQTVPDCRDPAFHEHFFFPVQ
  EEDDQKRLLVTVWNRASQSRQSGLIGCMSFGVKSLLTPDKEISGWYYLLGEHLGRTKHLKVARRRLRPLR
  DPLLRMPGGGDTENGKKLKITIPRGKDGFGFTICCDSPVRVQAVDSGGPAERAGLQQLDTVLQLNERPVE
  HWKCVELAHEIRSCPSEIILLVWRMVPQVKPGPDGGVLRRASCKSTHDLQSPPNKREKNCTHGVQARPEQ
  RHSCHLVCDSSDGLLLGGWERYTEVAKRGGQHTLPALSRATAPTDPNYIILAPLNPGSQLLRPVYQEDTI
  PEESGSPSKGKSYTGLGKKSRLMKTVQTMKGHGNYQNCPVVRPHATHSSYGTYVTLAPKVLVFPVFVQPL
  DLCNPARTLLLSEELLLYEGRNKAAEVTLFAYSDLLLFTKEDEPGRCDVLRNPLYLQSVKLQEGSSEDLK
  FCVLYLAEKAECLFTLEAHSQEQKKRVCWCLSENIAKQQQLAASPPDSKMFETEADEKREMALEEGKGPG
  AEDSPPSKEPSPGQELPPGQDLPPNKDSPSGQEPAPSQEPLSSKDSATSEGSPPGPDAPPSKDVPPCQEP
  PPAQDLSPCQDLPAGQEPLPHQDPLLTKDLPAIQESPTRDLPPCQDLPPSQVSLPAKALTEDTMSSGDLL
  AATGDPPAAPRPAFVIPEVRLDSTYSQKAGAEQGCSGDEEDAEEAEEVEEGEEGEEDEDEDTSDDNYGER
  SEAKRSSMIETGQGAEGGLSLRVQNSLRRRTHSEGSLLQEPRGPCFASDTTLHCSDGEGAASTWGMPSPS
  TLKKELGRNGGSMHHLSLFFTGHRKMSGADTVGDDDEASRKRKSKNLAKDMKNKLGIFRRRNESPGAPPA
  GKADKMMKSFKPTSEEALKWGESLEKLLVHKYGLAVFQAFLRTEFSEENLEFWLACEDFKKVKSQSKMAS
  KAKKIFAEYIAIQACKEVNLDSYTREHTKDNLQSVTRGCFDLAQKRIFGLMEKDSYPRFLRSDLYLDLIN
  QKKMSPPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144488
• ORF Size: 3594 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144488.4, NM_144488.5, NP_652759.3
• RefSeq Size: 4643 bp
• RefSeq ORF: 3597 bp
• Locus ID: 5998
• Cytogenetics: 9q32
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 132.2 kDa
• Gene Summary: 'This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]'