AP1S1 (NM_001283) Human Tagged ORF Clone

CAT#: RC224834

AP1S1 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, sigma 1 subunit (AP1S1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AP1S1
• Synonyms: AP19; CLAPS1; EKV3; MEDNIK; SIGMA1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224834 representing NM_001283
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCGGTTCATGCTATTATTCAGCCGGCAGGGAAAACTGCGGCTGCAAAAATGGTACCTGGCCACTT
  CGGACAAGGAACGGAAGAAGATGGTGCGCGAGCTCATGCAGGTTGTCCTGGCTCGAAAGCCCAAGATGTG
  CAGCTTCCTGGAGTGGAGGGACCTCAAAGTTGTCTATAAGAGATATGCCAGCCTCTACTTCTGCTGCGCC
  ATCGAGGGCCAAGACAATGAGCTCATCACACTGGAGCTGATCCACCGATACGTGGAGCTCTTAGACAAAT
  ACTTTGGCAGTGTGTGCGAGCTGGACATCATCTTCAACTTTGAGAAGGCCTACTTCATCCTGGATGAGTT
  TTTGATGGGGGGGGATGTCCAGGACACCTCCAAGAAGAGTGTGCTGAAAGCCATCGAGCAGGCTGACCTA
  CTGCAAGAGGAGGATGAGTCGCCACGGAGTGTGCTGGAGGAGATGGGTTTGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224834 representing NM_001283
  Red=Cloning site Green=Tags(s)
  
  MMRFMLLFSRQGKLRLQKWYLATSDKERKKMVRELMQVVLARKPKMCSFLEWRDLKVVYKRYASLYFCCA
  IEGQDNELITLELIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLMGGDVQDTSKKSVLKAIEQADL
  LQEEDESPRSVLEEMGLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001283
• ORF Size: 474 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001283.1, NM_001283.2, NM_001283.3, NP_001274.1
• RefSeq Size: 1297 bp
• RefSeq ORF: 477 bp
• Locus ID: 1174
• Cytogenetics: 7q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 18.7 kDa
• Gene Summary: 'The protein encoded by this gene is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle. [provided by RefSeq, Jul 2008]'