AP2 alpha (AP2A1) (NM_014203) Human Tagged ORF Clone
CAT#: RC224855
AP2A1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1
"NM_014203" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | AP2A1 |
Synonyms | ADTAA; AP2-ALPHA; CLAPA1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224855 representing NM_014203
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCCGTGTCCAAGGGCGATGGGATGCGGGGGCTCGCGGTGTTCATCTCCGACATCCGGAACTGTA AGAGCAAAGAGGCGGAAATTAAGAGAATCAACAAGGAACTGGCCAACATCCGCTCCAAGTTCAAAGGAGA CAAAGCCTTGGATGGCTACAGTAAGAAAAAATATGTGTGTAAACTGCTTTTCATCTTCCTGCTTGGCCAT GACATTGACTTTGGGCACATGGAGGCTGTGAATCTGTTGAGTTCCAATAAATACACAGAGAAGCAAATAG GTTACCTGTTCATTTCTGTGCTGGTGAACTCGAACTCGGAGCTGATCCGCCTCATCAACAACGCCATCAA GAATGACCTGGCCAGCCGCAACCCCACCTTCATGTGCCTGGCCCTGCACTGCATCGCCAACGTGGGCAGC CGGGAGATGGGCGAGGCCTTTGCCGCTGACATCCCCCGCATCCTGGTGGCCGGGGACAGCATGGACAGTG TCAAGCAGAGTGCGGCCCTGTGCCTCCTTCGACTGTACAAGGCCTCGCCTGACCTGGTGCCCATGGGCGA GTGGACGGCGCGTGTGGTACACCTGCTCAATGACCAGCACATGGGTGTGGTCACGGCCGCCGTCAGCCTC ATCACCTGTCTCTGCAAGAAGAACCCAGATGACTTCAAGACGTGCGTCTCTCTGGCTGTGTCGCGCCTGA GCCGGATCGTCTCCTCTGCCTCCACCGACCTCCAGGACTACACCTACTACTTCGTCCCAGCACCCTGGCT CTCGGTGAAGCTCCTGCGGCTGCTGCAGTGCTACCCGCCTCCAGAGGATGCGGCTGTGAAGGGGCGGCTG GTGGAATGTCTGGAGACTGTGCTCAACAAGGCCCAGGAGCCCCCCAAATCCAAGAAGGTGCAGCATTCCA ACGCCAAGAACGCCATCCTCTTCGAGACCATCAGCCTCATCATCCACTATGACAGTGAGCCCAACCTCCT GGTTCGGGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCACCGGGAGACCAACCTGCGCTACCTGGCCCTG GAGAGCATGTGCACGCTGGCCAGCTCCGAGTTCTCCCATGAAGCCGTCAAGACGCACATTGACACCGTCA TCAATGCCCTCAAGACGGAGCGGGACGTCAGCGTGCGGCAGCGGGCGGCTGACCTCCTCTACGCCATGTG TGACCGGAGCAATGCCAAGCAGATCGTGTCGGAGATGCTGCGGTACCTGGAGACGGCAGACTACGCCATC CGCGAGGAGATCGTCCTGAAGGTGGCCATCCTGGCCGAGAAGTACGCCGTGGACTACAGCTGGTACGTGG ACACCATCCTCAACCTCATCCGCATTGCGGGCGACTACGTGAGTGAGGAGGTGTGGTACCGTGTGCTACA GATCGTCACCAACCGTGATGACGTCCAGGGCTATGCCGCCAAGACCGTCTTTGAGGCGCTCCAGGCCCCT GCCTGTCACGAGAACATGGTGAAGGTTGGCGGCTACATCCTTGGGGAGTTTGGGAACCTGATTGCTGGGG ACCCCCGCTCCAGCCCCCCAGTGCAGTTCTCCCTGCTCCACTCCAAGTTCCATCTGTGCAGCGTGGCCAC GCGGGCGCTGCTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCCGAGACCAAGGCCACCATCCAG GGCGTCCTGCGGGCCGGCTCCCAGCTGCGCAATGCTGACGTGGAGCTGCAGCAGCGAGCCGTGGAGTACC TCACCCTCAGCTCAGTGGCCAGCACCGACGTCCTGGCCACGGTGCTGGAGGAGATGCCGCCCTTCCCCGA GCGCGAGTCGTCCATCCTGGCCAAGCTGAAACGCAAGAAGGGGCCAGGGGCCGGCAGCGCCCTGGACGAT GGCCGGAGGGACCCCAGCAGCAACGACATCAACGGGGGCATGGAGCCCACCCCCAGCACTGTGTCGACGC CCTCGCCCTCCGCCGACCTCCTGGGGCTGCGGGCAGCCCCTCCCCCGGCAGCACCCCCGGCTTCTGCAGG AGCAGGGAACCTTCTGGTGGACGTCTTCGATGGCCCGGCCGCCCAGCCCAGCCTGGGGCCCACCCCCGAG GAGGCCTTCCTCAGCGAGCTGGAGCCGCCTGCCCCCGAGAGCCCCATGGCTTTGCTGGCTGACCCAGCTC CAGCTGCTGACCCAGGTCCTGAGGACATCGGCCCTCCCATTCCGGAAGCCGATGAGTTGCTGAATAAGTT TGTGTGTAAGAACAACGGGGTCCTGTTCGAGAACCAGCTGCTGCAGATCGGAGTCAAGTCAGAGTTCCGA CAGAACCTGGGCCGCATGTATCTCTTCTATGGCAACAAGACCTCGGTGCAGTTCCAGAATTTCTCACCCA CTGTGGTTCACCCGGGAGACCTCCAGACTCAGCTGGCTGTGCAGACCAAGCGCGTGGCGGCGCAGGTGGA CGGCGGCGCGCAGGTGCAGCAGGTGCTCAATATCGAGTGCCTGCGGGACTTCCTGACGCCCCCGCTGCTG TCCGTGCGCTTCCGGTACGGTGGCGCCCCCCAGGCCCTCACCCTGAAGCTCCCAGTGACCATCAACAAGT TCTTCCAGCCCACCGAGATGGCGGCCCAGGATTTCTTCCAGCGCTGGAAGCAGCTGAGCCTCCCTCAACA GGAGGCGCAGAAAATCTTCAAAGCCAACCACCCCATGGACGCAGAAGTTACTAAGGCCAAGCTTCTGGGG TTTGGCTCTGCTCTCCTGGACAATGTGGACCCCAACCCTGAGAACTTCGTGGGGGCGGGGATCATCCAGA CTAAAGCCCTGCAGGTGGGCTGTCTGCTTCGGCTGGAGCCCAATGCCCAGGCCCAGATGTACCGGCTGAC CCTGCGCACCAGCAAGGAGCCCGTCTCCCGTCACCTGTGTGAGCTGCTGGCACAGCAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224855 representing NM_014203
Red=Cloning site Green=Tags(s) MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGH DIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGS REMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSL ITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRL VECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLAL ESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAP ACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQ GVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDD GRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPE EAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFR QNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLL SVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLG FGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014203 |
ORF Size | 2931 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014203.1, NM_014203.2, NP_055018.2 |
RefSeq Size | 3499 bp |
RefSeq ORF | 2934 bp |
Locus ID | 160 |
Cytogenetics | 19q13.33 |
Domains | Adaptin_N, Alpha_adaptin_C, Alpha_adaptinC2 |
Protein Pathways | Endocytosis, Huntington's disease |
MW | 107.4 kDa |
Gene Summary | 'This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC315023 | AP2A1 (untagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 |
USD 1,980.00 |
|
RG224855 | AP2A1 (GFP-tagged) - Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 |
USD 1,130.00 |
|
RC224855L3 | Lenti-ORF clone of AP2A1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 |
USD 1,230.00 |
|
RC224855L4 | Lenti-ORF clone of AP2A1 (mGFP-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 |
USD 1,230.00 |
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