AP2 alpha (AP2A1) (NM_014203) Human Untagged Clone

CAT#: SC315023

AP2A1 (untagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP2A1
• Synonyms: ADTAA; AP2-ALPHA; CLAPA1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014203 edited
  ATGCCGGCCGTGTCCAAGGGCGATGGGATGCGGGGGCTCGCGGTGTTCATCTCCGACATC
  CGGAACTGTAAGAGCAAAGAGGCGGAAATTAAGAGAATCAACAAGGAACTGGCCAACATC
  CGCTCCAAGTTCAAAGGAGACAAAGCCTTGGATGGCTACAGTAAGAAAAAATATGTGTGT
  AAACTGCTTTTCATCTTCCTGCTTGGCCATGACATTGACTTTGGGCACATGGAGGCTGTG
  AATCTGTTGAGTTCCAATAAATACACAGAGAAGCAAATAGGTTACCTGTTCATTTCTGTG
  CTGGTGAACTCGAACTCGGAGCTGATCCGCCTCATCAACAACGCCATCAAGAATGACCTG
  GCCAGCCGCAACCCCACCTTCATGTGCCTGGCCCTGCACTGCATCGCCAACGTGGGCAGC
  CGGGAGATGGGCGAGGCCTTTGCCGCTGACATCCCCCGCATCCTGGTGGCCGGGGACAGC
  ATGGACAGTGTCAAGCAGAGTGCGGCCCTGTGCCTCCTTCGACTGTACAAGGCCTCGCCT
  GACCTGGTGCCCATGGGCGAGTGGACGGCGCGTGTGGTACACCTGCTCAATGACCAGCAC
  ATGGGTGTGGTCACGGCCGCCGTCAGCCTCATCACCTGTCTCTGCAAGAAGAACCCAGAT
  GACTTCAAGACGTGCGTCTCTCTGGCTGTGTCGCGCCTGAGCCGGATCGTCTCCTCTGCC
  TCCACCGACCTCCAGGACTACACCTACTACTTCGTCCCAGCACCCTGGCTCTCGGTGAAG
  CTCCTGCGGCTGCTGCAGTGCTACCCGCCTCCAGAGGATGCGGCTGTGAAGGGGCGGCTG
  GTGGAATGTCTGGAGACTGTGCTCAACAAGGCCCAGGAGCCCCCCAAATCCAAGAAGGTG
  CAGCATTCCAACGCCAAGAACGCCATCCTCTTCGAGACCATCAGCCTCATCATCCACTAT
  GACAGTGAGCCCAACCTCCTGGTTCGGGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCAC
  CGGGAGACCAACCTGCGCTACCTGGCCCTGGAGAGCATGTGCACGCTGGCCAGCTCCGAG
  TTCTCCCATGAAGCCGTCAAGACGCACATTGACACCGTCATCAATGCCCTCAAGACGGAG
  CGGGACGTCAGCGTGCGGCAGCGGGCGGCTGACCTCCTCTACGCCATGTGTGACCGGAGC
  AATGCCAAGCAGATCGTGTCGGAGATGCTGCGGTACCTGGAGACGGCAGACTACGCCATC
  CGCGAGGAGATCGTCCTGAAGGTGGCCATCCTGGCCGAGAAGTACGCCGTGGACTATAGC
  TGGTACGTGGACACCATCCTCAACCTCATCCGCATTGCGGGCGACTACGTGAGTGAGGAG
  GTGTGGTACCGTGTGCTACAGATCGTCACCAACCGTGATGACGTCCAGGGCTATGCCGCC
  AAGACCGTCTTTGAGGCGCTCCAGGCCCCTGCCTGTCACGAGAACATGGTGAAGGTTGGC
  GGCTACATCCTTGGGGAGTTTGGGAACCTGATTGCTGGGGACCCCCGCTCCAGCCCCCCA
  GTGCAGTTCTCCCTGCTCCACTCCAAGTTCCATCTGTGCAGCGTGGCCACGCGGGCGCTG
  CTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCCGAGACCAAGGCCACCATCCAG
  GGCGTCCTGCGGGCCGGCTCCCAGCTGCGCAATGCTGACGTGGAGCTGCAGCAGCGAGCC
  GTGGAGTACCTCACCCTCAGCTCAGTGGCCAGCACCGACGTCCTGGCCACGGTGCTGGAG
  GAGATGCCGCCCTTCCCCGAGCGCGAGTCGTCCATCCTGGCCAAGCTGAAACGCAAGAAG
  GGGCCAGGGGCCGGCAGCGCCCTGGACGATGGCCGGAGGGACCCCAGCAGCAACGACATC
  AACGGGGGCATGGAGCCCACCCCCAGCACTGTGTCGACGCCCTCGCCCTCCGCCGACCTC
  CTGGGGCTGCGGGCAGCCCCTCCCCCGGCAGCACCCCCGGCTTCTGCAGGAGCAGGGAAC
  CTTCTGGTGGACGTCTTCGATGGCCCGGCCGCCCAGCCCAGCCTGGGGCCCACCCCCGAG
  GAGGCCTTCCTCAGCGAGCTGGAGCCGCCTGCCCCCGAGAGCCCCATGGCTTTGCTGGCT
  GACCCAGCTCCAGCTGCTGACCCAGGTCCTGAGGACATCGGCCCTCCCATTCCGGAAGCC
  GATGAGTTGCTGAATAAGTTTGTGTGTAAGAACAACGGGGTCCTGTTCGAGAACCAGCTG
  CTGCAGATCGGAGTCAAGTCAGAGTTCCGACAGAACCTGGGCCGCATGTATCTCTTCTAT
  GGCAACAAGACCTCGGTGCAGTTCCAGAATTTCTCACCCACTGTGGTTCACCCGGGAGAC
  CTCCAGACTCAGCTGGCTGTGCAGACCAAGCGCGTGGCGGCGCAGGTGGACGGCGGCGCG
  CAGGTGCAGCAGGTGCTCAATATCGAGTGCCTGCGGGACTTCCTGACGCCCCCGCTGCTG
  TCCGTGCGCTTCCGGTACGGTGGCGCCCCCCAGGCCCTCACCCTGAAGCTCCCAGTGACC
  ATCAACAAGTTCTTCCAGCCCACCGAGATGGCGGCCCAGGATTTCTTCCAGCGCTGGAAG
  CAGCTGAGCCTCCCTCAACAGGAGGCGCAGAAAATCTTCAAAGCCAACCACCCCATGGAC
  GCAGAAGTTACTAAGGCCAAGCTTCTGGGGTTTGGCTCTGCTCTCCTGGACAATGTGGAC
  CCCAACCCTGAGAACTTCGTGGGGGCGGGGATCATCCAGACTAAAGCCCTGCAGGTGGGC
  TGTCTGCTTCGGCTGGAGCCCAATGCCCAGGCCCAGATGTACCGGCTGACCCTGCGCACC
  AGCAAGGAGCCCGTCTCCCGTCACCTGTGTGAGCTGCTGGCACAGCAGTTCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_014203
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this clone has been fully sequenced and found one SNP within the protein associated with this reference, NM_014203.2. This SNP doesn't change amino acid.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014203.2, NP_055018.2
• RefSeq Size: 3499 bp
• RefSeq ORF: 2934 bp
• Locus ID: 160
• Cytogenetics: 19q13.33
• Domains: Adaptin_N, Alpha_adaptin_C, Alpha_adaptinC2
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: 'This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform of this protein.