Caldesmon (CALD1) (NM_033138) Human Tagged ORF Clone
CAT#: RC224908
- TrueORF®
CALD1 (Myc-DDK-tagged)-Human caldesmon 1 (CALD1), transcript variant 1
"NM_033138" in other vectors (2)
Product Images
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Other products for "CALD1"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CALD1 |
Synonyms | CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224908 representing NM_033138
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGATTTTGAGCGTCGCAGAGAACTTAGAAGGCAAAAGAGGGAGGAGATGCGACTCGAAGCAGAAA GAATCGCCTACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAACGGCGCCGCCGAGCCCGACA GGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGACAGGTGACCGACCAGGTGGAGGTGAATGCC CAGAACAGTGTGCCTGACGAGGAGGCCAAGACAACCACCACAAACACTCAAGTGGAAGGGGATGATGAGG CCGCATTCCTGGAGCGCCTGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTTCAGGAGGCTCTGGAGCG GCAGAAGGAGTTCGACCCAACAATAACAGATGCAAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGAC ACAGCAGAAAATGAAACTACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGAAAGATACGAGATAGAGG AAACAGAAACAGTCACCAAGTCCTACCAGAAGAATGATTGGAGGGATGCTGAAGAAAACAAGAAAGAAGA CAAGGAAAAGGAGGAGGAGGAAGAGGAGAAGCCAAAGCGAGGGAGCATTGGAGAAAATCAGGTAGAGGTG ATGGTGGAAGAGAAAACAACTGAAAGCCAGGAGGAAACAGTGGTAATGTCATTAAAAAATGGGCAGATCA GTTCAGAAGAGCCTAAACAAGAGGAGGAGAGGGAACAAGGTTCAGATGAGATTTCCCATCATGAAAAGAT GGAAGAGGAAGACAAGGAAAGAGCTGAGGCAGAGAGGGCAAGGTTGGAAGCAGAAGAAAGAGAAAGAATT AAAGCCGAGCAAGACAAAAAGATAGCAGATGAACGAGCAAGAATTGAAGCAGAAGAAAAAGCAGCTGCCC AAGAAAGAGAAAGGAGAGAGGCAGAAGAGAGGGAAAGGATGAGGGAGGAAGAGAAAAGGGCAGCAGAGGA GAGGCAGAGGATAAAGGAGGAAGAGAAAAGGGCAGCAGAGGAGAGGCAGAGGATAAAGGAGGAAGAGAAA AGGGCAGCAGAGGAGAGGCAGAGGATAAAAGAGGAAGAGAAAAGGGCAGCAGAGGAGAGGCAAAGGGCCA GGGCAGAGGAGGAAGAGAAGGCTAAGGTAGAAGAGCAGAAACGTAACAAGCAGCTAGAAGAGAAAAAACA TGCCATGCAAGAGACAAAGATAAAAGGGGAAAAGGTAGAACAGAAAATAGAAGGGAAATGGGTAAATGAA AAGAAAGCACAAGAAGATAAACTTCAGACAGCTGTCCTAAAGAAACAGGGAGAAGAGAAGGGAACTAAAG TGCAAGCTAAAAGAGAAAAGCTCCAAGAAGACAAGCCTACCTTCAAAAAAGAAGAGATCAAAGATGAAAA GATTAAAAAGGACAAAGAACCCAAAGAAGAAGTTAAGAGCTTCATGGATCGAAAGAAGGGATTTACAGAA GTTAAGTCGCAGAATGGAGAATTCATGACCCACAAACTTAAACATACTGAGAATACTTTCAGCCGCCCTG GAGGGAGGGCCAGCGTGGACACCAAGGAGGCTGAGGGCGCCCCCCAGGTGGAAGCCGGCAAAAGGCTGGA GGAGCTTCGTCGTCGTCGCGGGGAGACCGAGAGCGAAGAGTTCGAGAAGCTCAAACAGAAGCAGCAGGAG GCGGCTTTGGAGCTGGAGGAACTCAAGAAAAAGAGGGAGGAGAGAAGGAAGGTCCTGGAGGAGGAAGAGC AGAGGAGGAAGCAGGAGGAAGCCGATCGAAAACTCAGAGAGGAGGAAGAGAAGAGGAGGCTAAAGGAAGA GATTGAAAGGCGAAGAGCAGAAGCTGCTGAGAAACGCCAGAAGATGCCAGAAGATGGCTTGTCAGATGAC AAGAAACCATTCAAGTGTTTCACTCCTAAAGGTTCATCTCTCAAGATAGAAGAGCGAGCAGAATTTTTGA ATAAGTCTGTGCAGAAAAGCAGTGGTGTCAAATCGACCCATCAAGCAGCAATAGTCTCCAAGATTGACAG CAGACTGGAGCAGTATACCAGTGCAATTGAGGGAACAAAAAGCGCAAAACCTACAAAGCCGGCAGCCTCG GATCTTCCTGTTCCTGCTGAAGGTGTACGCAACATCAAGAGTATGTGGGAGAAAGGGAATGTGTTTTCAT CCCCCACTGCAGCAGGCACACCAAATAAGGAAACTGCTGGCTTGAAGGTAGGGGTTTCTAGCCGCATCAA TGAATGGCTAACTAAAACCCCAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA GACGTATCCAGCAAGCGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCACTTCCCCCACTAAGGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224908 representing NM_033138
Red=Cloning site Green=Tags(s) MDDFERRRELRRQKREEMRLEAERIAYQRNDDDEEEAARERRRRARQERLRQKQEEESLGQVTDQVEVNA QNSVPDEEAKTTTTNTQVEGDDEAAFLERLARREERRQKRLQEALERQKEFDPTITDASLSLPSRRMQND TAENETTEKEEKSESRQERYEIEETETVTKSYQKNDWRDAEENKKEDKEKEEEEEEKPKRGSIGENQVEV MVEEKTTESQEETVVMSLKNGQISSEEPKQEEEREQGSDEISHHEKMEEEDKERAEAERARLEAEERERI KAEQDKKIADERARIEAEEKAAAQERERREAEERERMREEEKRAAEERQRIKEEEKRAAEERQRIKEEEK RAAEERQRIKEEEKRAAEERQRARAEEEEKAKVEEQKRNKQLEEKKHAMQETKIKGEKVEQKIEGKWVNE KKAQEDKLQTAVLKKQGEEKGTKVQAKREKLQEDKPTFKKEEIKDEKIKKDKEPKEEVKSFMDRKKGFTE VKSQNGEFMTHKLKHTENTFSRPGGRASVDTKEAEGAPQVEAGKRLEELRRRRGETESEEFEKLKQKQQE AALELEELKKKREERRKVLEEEEQRRKQEEADRKLREEEEKRRLKEEIERRRAEAAEKRQKMPEDGLSDD KKPFKCFTPKGSSLKIEERAEFLNKSVQKSSGVKSTHQAAIVSKIDSRLEQYTSAIEGTKSAKPTKPAAS DLPVPAEGVRNIKSMWEKGNVFSSPTAAGTPNKETAGLKVGVSSRINEWLTKTPDGNKSPAPKPSDLRPG DVSSKRNLWEKQSVDKVTSPTKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_033138 |
ORF Size | 2379 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033138.1, NM_033138.2, NM_033138.3, NP_149129.2 |
RefSeq Size | 5241 bp |
RefSeq ORF | 2382 bp |
Locus ID | 800 |
Cytogenetics | 7q33 |
Domains | Caldesmon |
Protein Pathways | Vascular smooth muscle contraction |
MW | 93.2 kDa |
Gene Summary | 'This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]' |
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