Caldesmon (CALD1) (NM_033138) Human Untagged Clone

CAT#: SC127518

CALD1 (untagged)-Human caldesmon 1 (CALD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CALD1
• Synonyms: CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033138, the custom clone sequence may differ by one or more nucleotides
  
  
  GAATTCGGCACSAGCTTGCTCTCTGGCTGTGCTCCTGCTTAAAGAAATCAGTCCTTCCTTTCCGACTTAG
  TCCTCGGGAAGAAGTTTCAGACTACAAGGTATCATTGGAACATTTCAAGATCATCAAATCAAATTCCACA
  GGGATTGGTGACCAACCAGAAGGCTCAGACATCTGATTGCTGACCTGTCCAGACATCATCTGGTCTCCCT
  GAACCTGAAATCACACCATGGATGATTTTGAGCGTCGCAGAGAACTTAGAAGGCAAAAGAGGGAGGAGAT
  GCGACTCGAAGCAGAAAGAATCGCCTACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAACGG
  CGCCGCCGAGCCCGACAGGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGACAGGTGACCGACC
  AGGTGGAGGTGAATGCCCAGAACAGTGTGCCTGACGAGGAGGCCAAGACAACCACCACAAACACTCAAGT
  GGAAGGGGATGATGAGGCCGCATTCCTGGAGCGCCTGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTT
  CAGGAGGCTCTGGAGCGGCAGAAGGAGTTCGACCCAACAATAACAGATGCAAGTCTGTCGCTCCCAAGCA
  GAAGAATGCAAAATGACACAGCAGAAAATGAAACTACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGA
  AAGATACGAGATAGAGGAAACAGAAACAGTCACCAAGTCCTACCAGAAGAATGATTGGAGGGATGCTGAA
  GAAAACAAGAAAGAAGACAAGGAAAAGGAGGAGGAGGAAGAGGAGAAGCCAAAGCGAGGGAGCATTGGAG
  AAAATCAGGTAGAGGTGATGGTGGAAGAGAAAACAACTGAAAGCCAGGAGGAAACAGTGGTAATGTCATT
  AAAAAATGGGCAGATCAGTTCAGAAGAGCCTAAACAAGAGGAGGAGAGGGAACAAGGTTCAGATGAGATT
  TCCCATCATGAAAAGATGGAAGAGGAAGACAAGGAAAGAGCTGAGGCAGAGAGGGCAAGGTTGGAAGCAG
  AAGAAAGAGAAAGAATTAAAGCCGAGCAAGACAAAAAGATAGCAGATGAACGAGCAAGAATTGAAGCAGA
  AGAAAAAGCAGCTGCCCAAGAAAGAGAAAGGAGAGAGGCAGAAGAGAGGGAAAGGATGAGGGAGGAAGAG
  AAAAGGGCAGCAGAGGAGAGGCAGAGGATAAAGGAGGAAGAGAAAAGGGCAGCAGAGGAGAGGCAGAGGA
  TAAAGGAGGAAGAGAAAAGGGCAGCAGAGGAGAGGCAGAGGATAAAAGAGGAAGAGAAAAGGGCAGCAGA
  GGAGAGGCAAAGGGCCAGGGCAGAGGAGGAAGAGAAGGCTAAGGTAGAAGAGCAGAAACGTAACAAGCAG
  CTAGAAGAGAAAAAACGTGCCATGCAAGAGACAAAGATAAAAGGGGAAAAGGTAGAACAGAAAATAGAAG
  GGAAATGGGTAAATGAAAAGAAAGCACAAGAAGATAAACTTCAGACAGCTGTCCTAAAGAAACAGGGAGA
  AGAGAAGGGAACTAAAGTGCAAGCTAAAAGAGAAAAGCTCCAAGAAGACAAGCCTACCTTCAAAAAAGAA
  GAGATCAAAGATGAAAAGATTAAAAAGGACAAAGAACCCAAAGAAGAAGTTAAGAGCTTCATGGATCGAA
  AGAAGGGATTTACAGAAGTTAAGTCGCAGAATGGAGAATTCATGACCCACAAACTTAAACATACTGAGAA
  TACTTTCAGCCGCCCTGGAGGGAGGGCCAGCGTGGACACCAAGGAGGCTGAGGGCGCCCCCCAGGTGGAA
  GCCGGCAAAAGGCTGGAGGAGCTTCGTCGTCGTCGCGGGGAGACCGAGAGCGAAGAGTTCGAGAAGCTCA
  AACAGAAGCAGCAGGAGGCGGCTTTGGAGCTGGAGGAACTCAAGAAAAAGAGGGAGGAGAGAAGGAAGGT
  CCTGGAGGAGGAAGAGCAGAGGAGGAAGCAGGAGGAAGCCGATCGAAAACTCAGAGAGGAGGAAGAGAAG
  AGGAGGCTAAAGGAAGAGATTGAAAGGCGAAGAGCAGAAGCTGCTGAGAAACGCCAGAAGATGCCAGAAG
  ATGGCTTGTCAGATGACAAGAAACCATTCAAGTGTTTCACTCCTAAAGGTTCATCTCTCAAGATAGAAGA
  GCGAGCAGAATTTTTGAATAAGTCTGTGCAGAAAAGCAGTGGTGTCAAATCGACCCATCAAGCAGCAATA
  GTCTCCAAGATTGACAGCAGACTGGAGCAGTATACCAGTGCAATTGAGGGAACAAAAAGCGCAAAACCTA
  CAAAGCCGGCAGCCTCGGATCTTCCTGTTCCTGCTGAAGGTGTACGCAACATCAAGAGTATGTGGGAGAA
  AGGGAATGTGTTTTCATCCCCCACTGCAGCAGGCACACCAAATAAGGAAACTGCTGGCTTGAAGGTAGGG
  GTTTCTAGCCGCATCAATGAATGGCTAACTAAAACCCCAGATGGAAACAAGTCACCTGCTCCCAAACCTT
  CTGACTTGAGACCAGGAGACGTATCCAGCAAGCGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCAC
  TTCCCCCACTAAGGTTTGAGAC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033138 unedited
  GGCCGCGAATTCGGCACCAGCTTGCTCTCTGGCTGTGCTCCTGCTTAAAGAAATCAGTCC
  TTCCTTTCCGACTTAGTCCTCGGGAAGAAGTTTCAGACTACAAGGTATCATTGGAACATT
  TCAAGATCATCAAATCAAATTCCACAGGGATTGGTGACCAACCAGAAGGCTCAGACATCT
  GATTGCTGACCTGTCCAGACATCATCTGGTCTCCCTGAACCTGAAATCACACCATGGATG
  ATTTTGAGCGTCGCAGAGAACTTAGAAGGCAAAAGAGGGAGGAGATGCGACTCGAAGCAG
  AAAGAATCGCCTACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAACGGCGCC
  GCCGAGCCCGACAGGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGACAGGTGA
  CCGACCAGGTGGAGGTGAATGCCCAGAACAGTGTGCCTGACGAGGAGGCCAAGACAACCA
  CCACAAACACTCAAGTGGAAGGGGATGATGAGGCCGCATTCCTGGAGCGCCTGGCTCGGC
  GTGAGGAAAGACGCCAAANACGCCTTCAGGAGGCTCTGGAGCGGCAGAAGGAGTTCGACC
  CAACAATAACAGATGCAAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGACACAGCAG
  AAAATGAAACTACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGAAAGATACGAGATAG
  AGGAAACAGAAACAGTCACCAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033138 unedited
  CATTCGTCTGCACCGCGGCCCGCAATCTAGACCCGAGCTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTGCAGGAAGGAATTCCATTTATTGGGGATGCATTTTCACAATATATGTTTA
  TTGGAGCGATCCATTATCAGTGAAAAGTATCAAGTGTTTATAAAATTTTTAGGAATGGCA
  GATTCACAGAACATGCTAGTCAGCTTGCAGTTTTACCTTGTAAAGATAACAGAGAATTAT
  AGTCTAACCAGTAAACAAGGAATTTACTTTTCAAAAGATTAAATCCAAACTGAACAAAAT
  TTTACCCTAAAACTTACTCCATCCAAATATTGGAATAAAAGTCAGCAGTGATACATTCTT
  TTTTGAACTTTAGATTTTCTAGAAAAATATGTAATAGTGATCAGGAGGAGCTTTTGTTCA
  AAAGTACCACCAAGCAATGTTACCTTACCATAGGCCTTAATTCAAACTTTGATCCATTTC
  ACTCCAATGACGGGCGTCAATGCTACCTGGGACACTTGTATTTGTAAAATCTGATTTAGC
  TTATTGGAGACTTGTGCCTACTTTGTCATGAGGGTTTGACTTCTGCATTCTTCGNGGCTT
  TCCTTCCTTTGGCTTAGGTTTGTTAAAGCTAGAAGATTCCATTGCTCTTTACAGACTTAT
  GAGGAAAATACCCTTTGCATCGCAGAAGTCCCTTTTCAAGGCACCCCTGCCCTGGTTAGC
  TTTTTTTGGGAGAAACCTGCCGATTAACCACAACCATACTTCGGGCGGCTCCCTCCCGGT
  GTCACGAAACTGACCACCCCCACCCTTGTTAAATCCCCTTGACCATGAATCTATGCCACC
  CCCCTGTTGCACCCCCCTTTTATCAAATTTTTGCCCCCCCATTTGGCACCCTCTATTTTC
  TTCTGGGACACAATCATTCTCGGCCTTCACGACCCCCCGCACTCCTCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033138
• Insert Size: 4860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033138.2, NP_149129.2
• RefSeq Size: 5241 bp
• RefSeq ORF: 5241 bp
• Locus ID: 800
• Cytogenetics: 7q33
• Domains: Caldesmon
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: 'This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (1, also known as aorta h-CaD). It is predominantly expressed in smooth muscle tissues.