Keratin 12 (KRT12) (NM_000223) Human Tagged ORF Clone

CAT#: RC224946

KRT12 (Myc-DDK-tagged)-Human keratin 12 (KRT12)

Product Images

Western blot validation of overexpression lysate (Cat# LY424858) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224946 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KRT12 protein (Cat# TP324946). The protein was produced from HEK293T cells transfected with KRT12 cDNA clone (Cat# RC224946) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KRT12
• Synonyms: K12; MECD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224946 representing NM_000223
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTCTCCAACAACACCATGTCACTCTCAGTGCGCACCCCCGGACTGTCCCGGCGGCTCTCCTCGC
  AGAGTGTGATAGGCAGACCCAGGGGCATGTCTGCTTCCAGTGTTGGAAGTGGTTATGGGGGAAGTGCCTT
  TGGCTTTGGAGCCAGCTGTGGGGGAGGCTTTTCTGCTGCTTCCATGTTTGGTTCTAGTTCCGGCTTTGGG
  GGTGGCTCCGGAAGTTCCATGGCAGGAGGACTGGGTGCTGGTTATGGGAGAGCCCTGGGTGGAGGTAGCT
  TTGGAGGGCTGGGGATGGGATTTGGGGGCAGCCCAGGAGGTGGCTCTCTAGGTATTCTCTCGGGCAATGA
  TGGAGGCCTTCTTTCTGGATCAGAAAAAGAAACTATGCAAAATCTTAATGATAGATTAGCTTCCTACCTG
  GATAAGGTGCGAGCTCTAGAAGAGGCTAATACTGAGCTAGAAAATAAAATTCGAGAATGGTATGAAACAC
  GAGGAACTGGGACTGCAGATGCTTCACAGAGCGATTACAGCAAATATTATCCACTGATTGAAGACCTCAG
  GAATAAGATCATTTCAGCCAGCATTGGAAATGCCCAGCTCCTCTTGCAGATTGACAATGCGAGACTAGCT
  GCTGAGGACTTCAGGATGAAGTATGAGAATGAACTGGCCCTGCGCCAGGGCGTAGAGGCCGACATCAATG
  GCCTGCGCCGGGTGCTGGACGAGCTGACCCTGACCAGGACCGACCTGGAGATGCAGATCGAGAGCCTGAA
  CGAGGAGCTGGCCTACATGAAGAAGAACCACGAGGATGAGCTCCAAAGCTTCCGGGTGGGCGGCCCAGGC
  GAGGTCAGCGTAGAAATGGACGCTGCCCCCGGAGTGGACCTCACCAGGCTCCTCAATGATATGCGGGCGC
  AGTATGAAACCATCGCTGAGCAGAATCGGAAGGACGCTGAAGCCTGGTTCATTGAAAAGAGCGGGGAGCT
  CCGTAAGGAGATTAGCACCAACACCGAGCAGCTTCAGTCCAGCAAGAGCGAGGTCACCGACCTGCGTCGC
  GCCTTTCAGAACCTGGAGATCGAGCTACAGTCCCAGCTCGCCATGAAGAAATCCCTGGAGGACTCCTTGG
  CCGAAGCCGAGGGCGATTACTGCGCGCAGCTGTCCCAGGTGCAGCAGCTCATCAGCAACCTGGAGGCACA
  GCTGCTCCAGGTGCGCGCGGACGCAGAGCGCCAGAACGTGGACCACCAGCGGCTGCTGAATGTCAAGGCC
  CGCCTGGAGCTGGAGATTGAGACCTACCGCCGCCTGCTGGACGGGGAGGCCCAAGGTGATGGTTTGGAGG
  AAAGTTTATTTGTGACAGACTCCAAATCACAAGCACAGTCAACTGATTCCTCTAAAGACCCAACCAAAAC
  CCGAAAAATCAAGACAGTTGTGCAGGAGATGGTGAATGGTGAGGTGGTCTCATCTCAAGTTCAGGAAATT
  GAAGAACTAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224946 representing NM_000223
  Red=Cloning site Green=Tags(s)
  
  MDLSNNTMSLSVRTPGLSRRLSSQSVIGRPRGMSASSVGSGYGGSAFGFGASCGGGFSAASMFGSSSGFG
  GGSGSSMAGGLGAGYGRALGGGSFGGLGMGFGGSPGGGSLGILSGNDGGLLSGSEKETMQNLNDRLASYL
  DKVRALEEANTELENKIREWYETRGTGTADASQSDYSKYYPLIEDLRNKIISASIGNAQLLLQIDNARLA
  AEDFRMKYENELALRQGVEADINGLRRVLDELTLTRTDLEMQIESLNEELAYMKKNHEDELQSFRVGGPG
  EVSVEMDAAPGVDLTRLLNDMRAQYETIAEQNRKDAEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRR
  AFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKA
  RLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEI
  EELM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000223
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000223.1, NM_000223.2, NM_000223.3, NP_000214.1
• RefSeq Size: 1867 bp
• RefSeq ORF: 1485 bp
• Locus ID: 3859
• Cytogenetics: 17q21.2
• MW: 53.3 kDa
• Gene Summary: 'KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]'