KCTD15 (NM_001129994) Human Tagged ORF Clone

CAT#: RC225391

KCTD15 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 15 (KCTD15), transcript variant 2

Product Images

Coomassie blue staining of purified KCTD15 protein (Cat# TP325391). The protein was produced from HEK293T cells transfected with KCTD15 cDNA clone (Cat# RC225391) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCTD15
• Synonyms: MGC2628; MGC25497
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225391 representing NM_001129994
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCACCGCAAGGAGCGGCCGAGCGGGTCCTCGCTTCACACACACGGCAGCACCGGCACCGCGGAGG
  GAGGAAACATGTCCCGGCTGTCTCTCACCCGGTCGCCTGTGTCTCCCCTGGCTGCCCAGGGCATCCCCCT
  GCCAGCCCAGCTCACCAAGTCCAATGCACCTGTGCACATCGATGTGGGCGGCCACATGTACACCAGCAGC
  CTGGCCACGCTCACCAAGTACCCTGACTCCAGGATAAGCCGCCTCTTCAATGGCACTGAACCCATCGTCC
  TGGACAGTTTGAAGCAACATTATTTCATTGACCGGGATGGGGAGATTTTCCGCTACGTCCTGAGCTTCCT
  GCGGACGTCCAAGCTGCTGCTTCCGGATGACTTTAAGGACTTCAGTCTGCTGTACGAGGAGGCGCGCTAC
  TATCAGCTCCAGCCCATGGTGCGCGAGCTGGAGCGCTGGCAGCAGGAGCAGGAGCAGCGGCGCCGCAGCC
  GGGCCTGTGACTGCCTGGTGGTGCGCGTCACGCCCGACTTGGGCGAGCGGATCGCACTCAGCGGCGAGAA
  GGCCCTCATCGAGGAGGTCTTCCCCGAGACCGGAGACGTCATGTGCAACTCCGTCAACGCCGGCTGGAAC
  CAGGACCCCACGCACGTCATCCGCTTCCCGCTCAATGGCTACTGCCGGCTCAACTCGGTACAGGTCCTGG
  AGCGGCTGTTCCAGAGGGGTTTCAGCGTGGCTGCGTCCTGTGGGGGCGGTGTGGACTCCTCCCAGTTCAG
  CGAGTATGTGCTTTGCCGGGAGGAGCGGCGGCCGCAGCCCACCCCCACTGCTGTTCGAATCAAGCAGGAA
  CCCCTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225391 representing NM_001129994
  Red=Cloning site Green=Tags(s)
  
  MPHRKERPSGSSLHTHGSTGTAEGGNMSRLSLTRSPVSPLAAQGIPLPAQLTKSNAPVHIDVGGHMYTSS
  LATLTKYPDSRISRLFNGTEPIVLDSLKQHYFIDRDGEIFRYVLSFLRTSKLLLPDDFKDFSLLYEEARY
  YQLQPMVRELERWQQEQEQRRRSRACDCLVVRVTPDLGERIALSGEKALIEEVFPETGDVMCNSVNAGWN
  QDPTHVIRFPLNGYCRLNSVQVLERLFQRGFSVAASCGGGVDSSQFSEYVLCREERRPQPTPTAVRIKQE
  PLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001129994
• ORF Size: 849 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001129994.1, NP_001123466.1
• RefSeq ORF: 852
• Locus ID: 79047
• Protein Families: Ion Channels: Other
• MW: 31.8 kDa