HSPBP1 (NM_001130106) Human Tagged ORF Clone

CAT#: RC225535

HSPBP1 (Myc-DDK-tagged)-Human HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1 (HSPBP1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY427167) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225535 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HSPBP1
• Synonyms: FES1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225535 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGACGAAGGCTCAAGGGGGAGCCGCCTGCCCCTGGCGCTGCCCCCGGCCTCCCAGGGTTGCTCTT
  CAGGGGGCGGCGGCGGCGGCTCCTCGGCTGGGGGCTCGGGCAATTCCCGGCCCCCACGCAACCTCCAAGG
  CTTGCTGCAGATGGCCATCACCGCGGGCTCTGAAGAGCCAGACCCTCCTCCAGAACCGATGAGTGAGGAG
  AGGCGTCAGTGGCTGCAGGAGGCCATGTCGGCTGCCTTCCGAGGCCAGCGGGAGGAGGTGGAGCAGATGA
  AGAGCTGCCTCCGAGTGCTGTCACAGCCCATGCCCCCCACTGCTGGGGAGGCCGAGCAGGCGGCCGACCA
  GCAAGAGCGAGAGGGGGCCCTGGAGCTGCTGGCCGACCTGTGTGAGAACATGGACAATGCCGCAGACTTC
  TGCCAGCTGTCTGGCATGCACCTGCTGGTGGGCCGGTACCTGGAGGCGGGGGCTGCGGGACTGCGGTGGC
  GGGCGGCACAGCTCATCGGCACGTGCAGTCAGAACGTGGCAGCCATCCAGGAGCAGGTGCTGGGCCTGGG
  TGCCCTGCGTAAGCTGCTGCGGCTGCTGGACCGCGACGCCTGCGACACGGTGCGCGTCAAGGCCCTCTTC
  GCCATCTCCTGTCTGGTCCGAGAGCAGGAGGCTGGGCTGCTGCAGTTCCTCCGCCTGGACGGCTTCTCTG
  TGTTGATGAGGGCCATGCAGCAGCAGGTGCAGAAGCTCAAGGTCAAATCAGCATTCCTGCTGCAGAACCT
  GCTGGTGGGCCACCCTGAACACAAAGGGACCCTGTGCTCCATGGGGATGGTCCAGCAGCTGGTGGCCCTG
  GTGCGGACAGAGCACAGCCCCTTCCACGAGCACGTGCTTGGAGCCCTGTGCAGCCTGGTGACAGACTTTC
  CGCAGGGTGTGCGCGAGTGTCGGGAGCCGGAACTGGGCCTGGAGGAGCTCCTCCGCCACCGCTGTCAGCT
  GCTGCAGCAGCATGAGGAGTACCAGGAGGAGCTGGAGTTCTGTGAAAAGCTGCTACAGACCTGTTTCTCC
  AGCCCAGCGGACGACAGCATGGATCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225535 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSDEGSRGSRLPLALPPASQGCSSGGGGGGSSAGGSGNSRPPRNLQGLLQMAITAGSEEPDPPPEPMSEE
  RRQWLQEAMSAAFRGQREEVEQMKSCLRVLSQPMPPTAGEAEQAADQQEREGALELLADLCENMDNAADF
  CQLSGMHLLVGRYLEAGAAGLRWRAAQLIGTCSQNVAAIQEQVLGLGALRKLLRLLDRDACDTVRVKALF
  AISCLVREQEAGLLQFLRLDGFSVLMRAMQQQVQKLKVKSAFLLQNLLVGHPEHKGTLCSMGMVQQLVAL
  VRTEHSPFHEHVLGALCSLVTDFPQGVRECREPELGLEELLRHRCQLLQQHEEYQEELEFCEKLLQTCFS
  SPADDSMDR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130106
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130106.1, NP_001123578.1
• RefSeq Size: 1816 bp
• RefSeq ORF: 1080 bp
• Locus ID: 23640
• MW: 39.3 kDa
• Gene Summary: Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR. [UniProtKB/Swiss-Prot Function]