TOMM40 (NM_001128917) Human Tagged ORF Clone

CAT#: RC225543

TOMM40 (Myc-DDK-tagged)-Human translocase of outer mitochondrial membrane 40 homolog (yeast) (TOMM40), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427019) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225543 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TOMM40
• Synonyms: C19orf1; D19S1177E; PER-EC1; PEREC1; TOM40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225543 representing NM_001128917
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACGTGTTGGCTGCCAGCTCGCCGCCCGCAGGGCCGCCACCGCCGCCTGCGCCGGCCCTCGTGG
  GGCTGCCGCCACCTCCGCCCTCGCCGCCGGGCTTCACGCTGCCGCCGCTGGGAGGCAGCCTGGGCGCCGG
  CACCAGTACGAGTCGAAGTTCGGAACGGACCCCCGGGGCTGCAACCGCCAGCGCCTCAGGGGCCGCCGAG
  GATGGGGCCTGCGGCTGCCTGCCCAACCCGGGCACATTCGAGGAGTGCCACCGGAAGTGCAAGGAGCTGT
  TTCCCATTCAGATGGAGGGTGTCAAGCTCACAGTCAACAAAGGGTTGAGTAACCATTTTCAGGTCAACCA
  CACAGTAGCCCTCAGCACAATCGGGGAGTCCAACTACCACTTCGGGGTCACATATGTGGGGACAAAGCAG
  CTGAGTCCCACAGAGGCGTTCCCTGTACTGGTGGGTGACATGGACAACAGTGGCAGTCTCAACGCTCAGG
  TCATTCACCAGCTGGGCCCCGGTCTCAGGTCCAAGATGGCCATCCAGACCCAGCAGTCGAAGTTTGTGAA
  CTGGCAGGTGGACGGGGAGTATCGGGGCTCTGACTTCACAGCAGCCGTCACCCTGGGGAACCCAGACGTC
  CTCGTGGGTTCAGGAATCCTCGTAGCCCACTACCTCCAGAGCATCACGCCTTGCCTGGCCCTGGGTGGAG
  AGCTGGTCTACCACCGGCGGCCTGGAGAGGAGGGCACTGTCATGTCTCTAGCTGGGAAATACACATTGAA
  CAACTGGTTGGCAACGGTAACGTTGGGCCAGGCGGGCATGCACGCAACATACTACCACAAAGCCAGTGAC
  CAGCTGCAGGTGGGTGTGGAGTTTGAGGCCAGCACAAGGATGCAGGACACCAGCGTCTCCTTCGGGTACC
  AGCTGGACCTGCCCAAGGCCAACCTCCTCTTCAAAGGCTCTGTGGATAGCAACTGGATCGTGGGTGCCAC
  GCTGGAGAAGAAGCTCCCACCCCTGCCCCTGACACTGGCCCTTGGGGCCTTCCTGAATCACCGCAAGAAC
  AAGTTTCAGTGTGGCTTTGGCCTCACCATCGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225543 representing NM_001128917
  Red=Cloning site Green=Tags(s)
  
  MGNVLAASSPPAGPPPPPAPALVGLPPPPPSPPGFTLPPLGGSLGAGTSTSRSSERTPGAATASASGAAE
  DGACGCLPNPGTFEECHRKCKELFPIQMEGVKLTVNKGLSNHFQVNHTVALSTIGESNYHFGVTYVGTKQ
  LSPTEAFPVLVGDMDNSGSLNAQVIHQLGPGLRSKMAIQTQQSKFVNWQVDGEYRGSDFTAAVTLGNPDV
  LVGSGILVAHYLQSITPCLALGGELVYHRRPGEEGTVMSLAGKYTLNNWLATVTLGQAGMHATYYHKASD
  QLQVGVEFEASTRMQDTSVSFGYQLDLPKANLLFKGSVDSNWIVGATLEKKLPPLPLTLALGAFLNHRKN
  KFQCGFGLTIG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128917
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001128917.1, NP_001122389.1
• RefSeq ORF: 1086
• Locus ID: 10452
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• MW: 37.7 kDa
• Gene Summary: The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]