Cbl c (CBLC) (NM_001130852) Human Tagged ORF Clone

CAT#: RC225700

CBLC (Myc-DDK-tagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY427289) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225700 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CBLC
• Synonyms: CBL-3; CBL-SL; RNF57
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225700 representing NM_001130852
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGCGGTGGCCCCGTGGGGGCGACAGTGGGAAGAGGCCCGCGCCCTGGGCCGGGCAGTCAGGA
  TGCTGCAGCGCCTAGAAGAGCAATGCGTCGACCCCCGGCTGTCCGTGAGTCCCCCTTCGCTGCGGGACCT
  GCTGCCCCGCACAGCGCAGCTGCTTCGAGAGGTGGCCCATTCTCGGCGGGCGGCCGGCGGAGGCGGCCCC
  GGGGGTCCCGGCGGCTCTGGGGACTTTCTACTCATCTACCTGGCCAATCTGGAGGCCAAGAGCAGGCAGG
  TGGCCGCGCTGCTGCCTCCCCGGGGCCGAAGGAGTGCCAACGACGAGCTCTTCCGGGCGGGCTCCAGACT
  CAGGCGACAGCTGGCCAAGCTGGCCATCATCTTCAGCCACATGCACGCAGAGCTGCACGCACTCTTCCCC
  GGGGGAAAGTACTGTGGACACATGTACCAGCTCACCAAGGCCCCCGCCCACACCTTCTGGAGGGAAAGTT
  GCGGAGCCCGGTGTGTGCTGCCCTGGGCTGAGTTTGAGTCCCTCCTGGGCACCTGCCACCCTGTGGAACC
  AGGCTGCACAGCCCTGGCCTTGCGCACCACCATTGACCTCACCTGCAGCGGGCACGTGTCCATCTTCGAG
  TTCGACGTCTTCACCAGGCTCTTTCAGCCATGGCCAACACTCCTCAAGAACTGGCAGCTCCTGGCAGTCA
  ACCACCCAGGCTACATGGCCTTCCTCACCTATGATGAGGTCCAAGAGCGTCTGCAGGCCTGCAGGGACAA
  GCCAGGCAGCTACCTCTACCCAGATGGAAAGACCCACAACCCAGACCTGACTGAGCTCGGCCAGGCAGAA
  CCCCAGCAGCGCATCCACGTGTCAGAGGAGCAGCTGCAGCTCTACTGGGCCATGGACTCCACATTTGAGC
  TCTGCAAGATCTGTGCTGAGAGCAACAAGGATGTGAAGATTGAGCCGTGCGGGCACCTGCTCTGCAGCTG
  CTGCCTGGCTGCCTGGCAGCACTCGGACAGCCAGACCTGCCCCTTCTGCCGCTGCGAGATCAAGGGCTGG
  GAGGCCGTGAGTATCTACCAGTTCCACGGTCAGGCTACTGCTGAGGACTCAGGGAACAGCAGTGACCAGG
  AAGGCAGGGAGTTGGAGCTGGGGCAGGTGCCCCTTTCGGCTCCTCCATTGCCCCCACGGCCAGATCTGCC
  CCCCAGGAAGCCCAGAAATGCCCAGCCGAAAGTGAGACTCCTAAAGGGGAACTCCCCTCCAGCTGCGCTG
  GGACCCCAGGACCCTGCCCCGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225700 representing NM_001130852
  Red=Cloning site Green=Tags(s)
  
  MALAVAPWGRQWEEARALGRAVRMLQRLEEQCVDPRLSVSPPSLRDLLPRTAQLLREVAHSRRAAGGGGP
  GGPGGSGDFLLIYLANLEAKSRQVAALLPPRGRRSANDELFRAGSRLRRQLAKLAIIFSHMHAELHALFP
  GGKYCGHMYQLTKAPAHTFWRESCGARCVLPWAEFESLLGTCHPVEPGCTALALRTTIDLTCSGHVSIFE
  FDVFTRLFQPWPTLLKNWQLLAVNHPGYMAFLTYDEVQERLQACRDKPGSYLYPDGKTHNPDLTELGQAE
  PQQRIHVSEEQLQLYWAMDSTFELCKICAESNKDVKIEPCGHLLCSCCLAAWQHSDSQTCPFCRCEIKGW
  EAVSIYQFHGQATAEDSGNSSDQEGRELELGQVPLSAPPLPPRPDLPPRKPRNAQPKVRLLKGNSPPAAL
  GPQDPAPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130852
• ORF Size: 1284 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001130852.1, NP_001124324.1
• RefSeq ORF: 1287
• Locus ID: 23624
• Protein Families: Druggable Genome
• Protein Pathways: Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
• MW: 47.2 kDa
• Gene Summary: This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]