MANEL (MANEAL) (NM_001113482) Human Tagged ORF Clone

CAT#: RC225767

MANEAL (Myc-DDK-tagged)-Human mannosidase, endo-alpha-like (MANEAL), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY426415) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225767 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MANEAL protein (Cat# TP325767). The protein was produced from HEK293T cells transfected with MANEAL cDNA clone (Cat# RC225767) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MANEAL
• Synonyms: RP11-109P14.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225767 representing NM_001113482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGCGGCGGCGCCGCGCCTGCATCGCTCTGTTCCTGGTGCTGCTCTTCGCCTTCGGCACCCTCA
  TGGGTCTGCGCACGCTCAAGGCTCCGGACGGACTCCCGGCGCTGGGCCCGGGCCTGGAGCTGGCGCCCTT
  TGAGCGACGCCCAGAGGGGGCCCCCGCGCCCGCTGCCAGGGCCCCGGCAGCCCCTGCCGCGCCGCCCCCG
  CCGCCGCCGCCGCCCCGCACCGCGGACCCTGGCGGCTCCCCTGGGCCGGCACCCGCGGAGGCCGAGCCCG
  CCCCCGTGCAGAGCCTGCGCGTCTACTCGGACCTGCACGCCTTCTACTACTCGTGGTACGGGAGCCCGCG
  GCGCGAGGGCCACTACATTCACTGGGACCACGTCATGGTGCCGCACTGGGACCCCAAGATCTCGGCCAGC
  TACCCCCGCGGCCGCCACAGCCCCCCAGACGACTTGGGCTCCAGCTTCTACCCGGAGCTGGGGCCCTACA
  GCTCCCGGGACCCCGAAGTGCTGCGGGAGCATATGACCCAGCTCAAGGAAGCCGCCATCGGCGTCCTGGT
  CCTGTCCTGGTACCCACCTGGCATGGCTGATGATAACGGGGAGCCCTCAGATGACCTGGTGCCCGCCATT
  CTGGACACCGCCCATCAGTACAGCATCCAGGTGGCCTTCCACATCCAACCCTACAAGGGCCGGGATGACA
  TCACTGTACATGACAACATCAAGTACATCATTGACACGTATGGCTCCCATGGTGCATTTTACCGCTATAA
  GAACAGCATGGGCAAGAGCCTCCCACTCTTTTATATCTACGACTCATACCTGACGTCCCCTGAGGCCTGG
  GCCCACCTCCTGACACCAAACGGGCCCCATTCGATCCGCAACACGCCCTACGATGGGGTCTTCATAGCGC
  TGCTGGTGGAGGAGGGCCACACCCACGATATCCTGGCCGCCGGATTTGACGGCATGTACACCTACTTTGC
  CTCCAATGGTTTCTCCTTTGGTTCTTCCCATCAGAACTGGAAAGCTGTGAAGAACTTTTGTGATGCCAAC
  AACCTCATGTTCATCCCCAGTGTGGGGCCTGGCTACATAGACACCAGCATTCGGCCCTGGAACAACCACA
  ATACGCGCAACAGGGTCAATGGCAAGTACTATGAGACGGCCCTGCAGGCGGCCCTGACAGTGAGGCCCGA
  GATCGTTTCCATTACCTCCTTCAATGAGTGGCACGAGGGCACCCAGATTGAGAAGGCCATTCCCAAGAAG
  ACACCCACCCGCCTGTATTTGGACTACCTGCCTCACCAGCCCAGCCTGTACCTGGAGCTGACACGCCGCT
  GGGCGGAGCACTTCATCAAAGAGAAGGAGCAGTGGCTCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225767 representing NM_001113482
  Red=Cloning site Green=Tags(s)
  
  MARRRRRACIALFLVLLFAFGTLMGLRTLKAPDGLPALGPGLELAPFERRPEGAPAPAARAPAAPAAPPP
  PPPPPRTADPGGSPGPAPAEAEPAPVQSLRVYSDLHAFYYSWYGSPRREGHYIHWDHVMVPHWDPKISAS
  YPRGRHSPPDDLGSSFYPELGPYSSRDPEVLREHMTQLKEAAIGVLVLSWYPPGMADDNGEPSDDLVPAI
  LDTAHQYSIQVAFHIQPYKGRDDITVHDNIKYIIDTYGSHGAFYRYKNSMGKSLPLFYIYDSYLTSPEAW
  AHLLTPNGPHSIRNTPYDGVFIALLVEEGHTHDILAAGFDGMYTYFASNGFSFGSSHQNWKAVKNFCDAN
  NLMFIPSVGPGYIDTSIRPWNNHNTRNRVNGKYYETALQAALTVRPEIVSITSFNEWHEGTQIEKAIPKK
  TPTRLYLDYLPHQPSLYLELTRRWAEHFIKEKEQWLM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113482
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113482.1, NP_001106954.1
• RefSeq ORF: 1374
• Locus ID: 149175
• Protein Families: Transmembrane
• MW: 51.1 kDa