SMAD9 (NM_001127217) Human Tagged ORF Clone

CAT#: RC225792

SMAD9 (Myc-DDK-tagged)-Human SMAD family member 9 (SMAD9), transcript variant a

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMAD9
• Synonyms: MADH6; MADH9; PPH2; SMAD8; SMAD8/9; SMAD8A; SMAD8B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225792 representing NM_001127217.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCACTCCACCACCCCCATCAGCTCCCTCTTCTCCTTCACCAGCCCCGCAGTGAAGAGACTGCTAGGC
  TGGAAGCAAGGAGATGAAGAGGAAAAGTGGGCAGAGAAGGCAGTGGACTCTCTAGTGAAGAAGTTAAAG
  AAGAAGAAGGGAGCCATGGACGAGCTGGAGAGGGCTCTCAGCTGCCCGGGGCAGCCCAGCAAATGCGTC
  ACGATTCCCCGCTCCCTGGACGGGCGGCTGCAGGTGTCCCACCGCAAGGGCCTGCCCCATGTGATTTAC
  TGTCGCGTGTGGCGCTGGCCGGATCTGCAGTCCCACCACGAGCTGAAGCCGCTGGAGTGCTGTGAGTTC
  CCATTTGGCTCCAAGCAGAAAGAAGTGTGCATTAACCCTTACCACTACCGCCGGGTGGAGACTCCAGTA
  CTGCCTCCTGTGCTCGTGCCAAGACACAGTGAATATAACCCCCAGCTCAGCCTCCTGGCCAAGTTCCGC
  AGCGCCTCCCTGCACAGTGAGCCACTCATGCCACACAACGCCACCTATCCTGACTCTTTCCAGCAGCCT
  CCGTGCTCTGCACTCCCTCCCTCACCCAGCCACGCGTTCTCCCAGTCCCCGTGCACGGCCAGCTACCCT
  CACTCCCCAGGAAGTCCTTCTGAGCCAGAGAGTCCCTATCAACACTCAGTTGACACACCACCCCTGCCT
  TATCATGCCACAGAAGCCTCTGAGACCCAGAGTGGCCAACCTGTAGATGCCACAGCTGATAGACATGTA
  GTGCTATCGATACCAAATGGAGACTTTCGACCAGTTTGTTACGAGGAGCCCCAGCACTGGTGCTCGGTC
  GCCTACTATGAACTGAACAACCGAGTTGGGGAGACATTCCAGGCTTCCTCCCGAAGTGTGCTCATAGAT
  GGGTTCACCGACCCTTCAAATAACAGGAACAGATTCTGTCTTGGACTTCTTTCTAATGTAAACAGAAAC
  TCAACGATAGAAAATACCAGGAGACATATAGGAAAGGGTGTGCACTTGTACTACGTCGGGGGAGAGGTG
  TATGCCGAGTGCGTGAGTGACAGCAGCATCTTTGTGCAGAGCCGGAACTGCAACTATCAACACGGCTTC
  CACCCAGCTACCGTCTGCAAGATCCCCAGCGGCTGCAGCCTCAAGGTCTTCAACAACCAGCTCTTCGCT
  CAGCTCCTGGCCCAGTCAGTTCACCACGGCTTTGAAGTCGTGTATGAACTGACCAAGATGTGTACTATC
  CGGATGAGTTTTGTTAAGGGTTGGGGTGCTGAGTATCATCGCCAGGATGTCACCAGCACCCCCTGCTGG
  ATTGAGATTCATCTTCATGGGCCACTGCAGTGGCTGGACAAAGTTCTGACTCAGATGGGCTCTCCACAT
  AACCCCATTTCTTCAGTGTCT
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC225792
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MHSTTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDSLVKKLKKKKGAMDELERALSCPGQPSKCV
  TIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYRRVETPV
  LPPVLVPRHSEYNPQLSLLAKFRSASLHSEPLMPHNATYPDSFQQPPCSALPPSPSHAFSQSPCTASYP
  HSPGSPSEPESPYQHSVDTPPLPYHATEASETQSGQPVDATADRHVVLSIPNGDFRPVCYEEPQHWCSV
  AYYELNNRVGETFQASSRSVLIDGFTDPSNNRNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEV
  YAECVSDSSIFVQSRNCNYQHGFHPATVCKIPSGCSLKVFNNQLFAQLLAQSVHHGFEVVYELTKMCTI
  RMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127217
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127217.3
• RefSeq ORF: 1404 bp
• Locus ID: 4093
• UniProt ID: O15198
• Cytogenetics: 13q13.3
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• MW: 52.5 kDa
• Gene Summary: The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]