SMAD9 (NM_001127217) Human Untagged Clone

CAT#: SC323084

SMAD9 (untagged)-Human SMAD family member 9 (SMAD9), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMAD9
• Synonyms: MADH6; MADH9; PPH2; SMAD8; SMAD8/9; SMAD8A; SMAD8B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001127217 edited
  ATGCACTCCACCACCCCCATCAGCTCCCTCTTCTCCTTCACCAGCCCCGCAGTGAAGAGA
  CTGCTAGGCTGGAAGCAAGGAGATGAAGAGGAAAAGTGGGCAGAGAAGGCAGTGGACTCT
  CTAGTGAAGAAGTTAAAGAAGAAGAAGGGAGCCATGGACGAGCTGGAGAGGGCTCTCAGC
  TGCCCGGGGCAGCCCAGCAAATGCGTCACGATTCCCCGCTCCCTGGACGGGCGGCTGCAG
  GTGTCCCACCGCAAGGGCCTGCCCCATGTGATTTACTGTCGCGTGTGGCGCTGGCCGGAT
  CTGCAGTCCCACCACGAGCTGAAGCCGCTGGAGTGCTGTGAGTTCCCATTTGGCTCCAAG
  CAGAAAGAAGTGTGCATTAACCCTTACCACTACCGCCGGGTGGAGACTCCAGTACTGCCT
  CCTGTGCTCGTGCCAAGACACAGTGAATATAACCCCCAGCTCAGCCTCCTGGCCAAGTTC
  CGCAGCGCCTCCCTGCACAGTGAGCCACTCATGCCACACAACGCCACCTATCCTGACTCT
  TTCCAGCAGCCTCCGTGCTCTGCACTCCCTCCCTCACCCAGCCACGCGTTCTCCCAGTCC
  CCGTGCACGGCCAGCTACCCTCACTCCCCAGGAAGTCCTTCTGAGCCAGAGAGTCCCTAT
  CAACACTCAGTTGACACACCACCCCTGCCTTATCATGCCACAGAAGCCTCTGAGACCCAG
  AGTGGCCAACCTGTAGATGCCACAGCTGATAGACATGTAGTGCTATCGATACCAAATGGA
  GACTTTCGACCAGTTTGTTACGAGGAGCCCCAGCACTGGTGCTCGGTCGCCTACTATGAA
  CTGAACAACCGAGTTGGGGAGACATTCCAGGCTTCCTCCCGAAGTGTGCTCATAGATGGG
  TTCACCGACCCTTCAAATAACAGGAACAGATTCTGTCTTGGACTTCTTTCTAATGTAAAC
  AGAAACTCAACGATAGAAAATACCAGGAGACATATAGGAAAGGGTGTGCACTTGTACTAC
  GTCGGGGGAGAGGTGTATGCCGAGTGCGTGAGTGACAGCAGCATCTTTGTGCAGAGCCGG
  AACTGCAACTATCAACACGGCTTCCACCCAGCTACCGTCTGCAAGATCCCCAGCGGCTGC
  AGCCTCAAGGTCTTCAACAACCAGCTCTTCGCTCAGCTCCTGGCCCAGTCAGTTCACCAC
  GGCTTTGAAGTCGTGTATGAACTGACCAAGATGTGTACTATCCGGATGAGTTTTGTTAAG
  GGTTGGGGTGCTGAGTATCATCGCCAGGATGTCACCAGCACCCCCTGCTGGATTGAGATT
  CATCTTCATGGGCCACTGCAGTGGCTGGACAAAGTTCTGACTCAGATGGGCTCTCCACAT
  AACCCCATTTCTTCAGTGTCTTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001127217
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127217.1, NP_001120689.1
• RefSeq Size: 2365 bp
• RefSeq ORF: 1404 bp
• Locus ID: 4093
• Cytogenetics: 13q13.3
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (a) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.