ATF7 (NM_001130059) Human Tagged ORF Clone

CAT#: RC225852

ATF7 (Myc-DDK-tagged)-Human activating transcription factor 7 (ATF7), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427137) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225852 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ATF7 protein (Cat# TP325852). The protein was produced from HEK293T cells transfected with ATF7 cDNA clone (Cat# RC225852) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATF7
• Synonyms: ATFA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225852 representing NM_001130059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACGACAGACCGTTTGTGTGCAATGCCCCGGGCTGTGGACAGAGATTTACAAACGAGGACCACC
  TGGCAGTTCATAAACACAAGCATGAGATGACATTGAAATTTGGCCCAGCCCGAACTGACTCAGTCATCAT
  TGCAGATCAAACGCCTACTCCAACTAGATTCCTGAAGAACTGTGAGGAGGTGGGACTCTTCAATGAACTA
  GCTAGCTCCTTTGAACATGAATTCAAGAAAGCTGCAGATGAGGATGAGAAAAAGGCAAGAAGCAGGACTG
  TTGCCAAAAAACTGGTGGCTGCTGCTGGGCCCCTTGACATGTCTCTGCCTTCCACACCAGACATCAAAAT
  CAAAGAAGAAGAGCCAGTGGAGGTAGACTCATCCCCACCTGATAGCCCTGCCTCTAGTCCCTGTTCCCCA
  CCACTGAAGGAGAAGGAGGTTACCCCAAAGCCTGTTCTGATCTCTACCCCCACACCCACCATTGTACGTC
  CTGGCTCCCTGCCTCTCCACTTGGGCTATGATCCACTTCATCCAACCCTTCCCTCCCCAACCTCTGTCAT
  CACACAGGCTCCACCATCCAACAGGCAAATGGGGTCTCCCACTGGCTCCCTCCCTCTTGTCATGCATCTT
  GCTAATGGACAGACCATGCCTGTGTTGCCAGGGCCTCCAGTACAGATGCCGTCTGTTATATCGCTGGCCA
  GACCTGTGTCCATGGTGCCCAACATTCCTGGTATCCCTGGCCCACCAGTTAACAGTAGTGGCTCCATTTC
  TCCCTCTGGCCACCCTATACCATCAGAAGCCAAGATGAGACTGAAAGCCACCCTAACTCACCAAGTCTCC
  TCAATCAATGGTGGTTGTGGAATGGTGGTGGGTACTGCCAGCACCATGGTGACAGCCCGCCCAGAGCAGA
  GCCAGATTCTCATCCAGCACCCTGATGCCCCATCCCCTGCCCAGCCACAGGTCTCACCAGCTCAGCCCAC
  CCCTAGTACTGGGGGGCGACGGCGGCGCACAGTAGATGAAGATCCAGATGAGCGACGGCAGCGCTTTCTG
  GAGCGCAACCGGGCTGCAGCCTCCCGCTGCCGCCAAAAGCGAAAGCTGTGGGTGTCCTCCCTAGAGAAGA
  AGGCCGAAGAACTCACTTCTCAGAACATTCAGCTGAGTAATGAAGTCACATTACTACGCAATGAGGTGGC
  CCAGTTGAAACAGCTACTGTTAGCTCATAAAGACTGCCCAGTCACTGCACTACAGAAAAAGACTCAAGGC
  TATTTAGAAAGCCCCAAGGAAAGCTCAGAGCCAACGGGTTCTCCAGCCCCTGTGATTCAGCACAGCTCAG
  CAACAGCCCCTAGCAATGGCCTCAGTGTTCGCTCTGCAGCTGAAGCTGTGGCCACCTCGGTCCTCACTCA
  GATGGCCAGCCAAAGGACAGAACTGAGCATGCCGATACAATCGCATGTAATCATGACCCCACAGTCCCAG
  TCTGCGGGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225852 representing NM_001130059
  Red=Cloning site Green=Tags(s)
  
  MGDDRPFVCNAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARTDSVIIADQTPTPTRFLKNCEEVGLFNEL
  ASSFEHEFKKAADEDEKKARSRTVAKKLVAAAGPLDMSLPSTPDIKIKEEEPVEVDSSPPDSPASSPCSP
  PLKEKEVTPKPVLISTPTPTIVRPGSLPLHLGYDPLHPTLPSPTSVITQAPPSNRQMGSPTGSLPLVMHL
  ANGQTMPVLPGPPVQMPSVISLARPVSMVPNIPGIPGPPVNSSGSISPSGHPIPSEAKMRLKATLTHQVS
  SINGGCGMVVGTASTMVTARPEQSQILIQHPDAPSPAQPQVSPAQPTPSTGGRRRRTVDEDPDERRQRFL
  ERNRAAASRCRQKRKLWVSSLEKKAEELTSQNIQLSNEVTLLRNEVAQLKQLLLAHKDCPVTALQKKTQG
  YLESPKESSEPTGSPAPVIQHSSATAPSNGLSVRSAAEAVATSVLTQMASQRTELSMPIQSHVIMTPQSQ
  SAGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130059
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130059.1, NP_001123531.1
• RefSeq ORF: 1484
• Locus ID: 11016
• Protein Families: Transcription Factors
• MW: 52.8 kDa
• Gene Summary: Isoform 5 acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation. [UniProtKB/Swiss-Prot Function]