ASH2L (NM_001105214) Human Tagged ORF Clone

CAT#: RC225918

ASH2L (Myc-DDK-tagged)-Human ash2 (absent, small, or homeotic)-like (Drosophila) (ASH2L), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASH2L
• Synonyms: ASH2; ASH2L1; ASH2L2; Bre2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225918 representing NM_001105214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACTCAGGCGGGCTCCGTGGATGAAGAGAATGGCCGACAGTTGGGTGAGGTAGAGCTGCAATGTG
  GGATTTGTACAAAATGGTTCACGGCTGACACATTTGGCATAGATACCTCATCCTGTCTACCTTTCATGAC
  CAACTACAGTTTTCATTGCAACGTCTGCCATCACAGTGGGAATACCTATTTCCTCCGGAAGCAAGCAAAC
  TTGAAGGAAATGTGCCTTAGTGCTTTGGCCAACCTGACATGGCAGTCCCGAACACAGGATGAACATCCGA
  AGACAATGTTCTCCAAAGATAAGGATATTATACCATTTATTGATAAATACTGGGAGTGCATGACAACCAG
  ACAGAGACCTGGGAAAATGACTTGGCCAAATAACATTGTTAAAACAATGAGTAAAGAAAGAGATGTATTC
  TTGGTAAAGGAACACCCAGATCCAGGCAGTAAAGATCCAGAAGAAGATTACCCCAAATTTGGACTTTTGG
  ATCAGGACCTTAGTAACATTGGTCCTGCTTATGACAACCAAAAACAGAGCAGTGCTGTGTCTACTAGTGG
  GAATTTAAATGGGGGAATTGCAGCAGGAAGCAGCGGAAAAGGACGAGGAGCCAAGCGCAAACAGCAGGAT
  GGAGGGACCACAGGGACCACCAAGAAGGCCCGGAGTGACCCTTTGTTTTCTGCTCAGCGCCTTCCCCCTC
  ATGGCTACCCATTGGAACACCCGTTTAACAAAGATGGCTATCGGTATATTCTAGCTGAGCCTGATCCGCA
  CGCCCCTGACCCCGAGAAGCTGGAACTTGACTGCTGGGCAGGAAAACCTATTCCTGGAGACCTCTACAGA
  GCCTGCTTGTATGAACGGGTTTTGTTAGCCCTACATGATCGAGCTCCCCAGTTAAAGATCTCAGATGACC
  GGCTGACTGTGGTTGGAGAGAAGGGCTACTCTATGGTGAGGGCCTCTCATGGAGTACGGAAAGGTGCCTG
  GTATTTTGAAATCACTGTGGATGAGATGCCACCAGATACCGCTGCCAGACTGGGTTGGTCCCAGCCCCTA
  GGAAACCTTCAAGCTCCTTTAGGTTATGATAAATTTAGCTATTCTTGGCGGAGCAAAAAGGGAACCAAGT
  TCCACCAGTCCATTGGCAAACACTACTCTTCTGGCTATGGACAGGGAGACGTCCTGGGATTTTATATTAA
  TCTTCCTGAAGACACAGAGACAGCCAAGTCATTGCCAGACACATACAAAGATAAGGCTTTGATAAAATTC
  AAGAGTTATTTGTATTTTGAGGAAAAAGACTTTGTGGATAAAGCAGAGAAGAGCCTGAAGCAGACTCCCC
  ATAGTGAGATAATATTTTATAAAAATGGTGTCAATCAAGGTGTGGCTTACAAAGATATTTTTGAGGGGGT
  TTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACGGTTTCCATTAACTTTGGACCATGCTTCAAGTAT
  CCTCCGAAGGATCTCACTTACCGCCCTATGAGTGACATGGGCTGGGGCGCCGTGGTAGAGCACACCCTGG
  CTGACGTCTTGTATCACGTGGAGACAGAAGTGGATGGGAGGCGCAGTCCCCCATGGGAACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225918 representing NM_001105214
  Red=Cloning site Green=Tags(s)
  
  MDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFMTNYSFHCNVCHHSGNTYFLRKQAN
  LKEMCLSALANLTWQSRTQDEHPKTMFSKDKDIIPFIDKYWECMTTRQRPGKMTWPNNIVKTMSKERDVF
  LVKEHPDPGSKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSTSGNLNGGIAAGSSGKGRGAKRKQQD
  GGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYR
  ACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEMPPDTAARLGWSQPL
  GNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLPEDTETAKSLPDTYKDKALIKF
  KSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKY
  PPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105214
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001105214.1, NM_001105214.2, NP_001098684.1
• RefSeq Size: 3055
• RefSeq ORF: 1605
• Locus ID: 9070
• Protein Families: Druggable Genome, Transcription Factors
• MW: 60.2 kDa
• Gene Summary: Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. [UniProtKB/Swiss-Prot Function]