KLC2 (NM_001134774) Human Tagged ORF Clone

CAT#: RC225936

KLC2 (Myc-DDK-tagged)-Human kinesin light chain 2 (KLC2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY427490) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225936 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLC2
• Synonyms: SPOAN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225936 representing NM_001134774
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGATGGTGTTTCCGCGGGAGGAGAAGCTGAGCCAGGATGAGATCGTGCTGGGCACCAAGGCTG
  TCATCCAGGGACTGGAGACTCTGCGTGGGGAGCATCGTGCCCTGCTGGCTCCTCTGGTTGCACCTGAGGC
  CGGCGAAGCCGAGCCTGGCTCGCAGGAGCGCTGCATCCTCCTGCGTCGCTCCCTGGAAGCCATTGAGCTT
  GGGCTGGGGGAGGCCCAGGAGGAGAAGGGGGACGTCCCCAAAGACACACTGGATGACCTGTTCCCCAATG
  AGGATGAGCAGAGCCCAGCCCCTAGCCCAGGAGGAGGGGATGTGTCTGGTCAGCATGGGGGCTACGAGAT
  CCCGGCCCGGCTCCGCACCCTGCACAACCTGGTGATCCAATACGCCTCACAGGGCCGCTACGAGGTAGCT
  GTGCCACTCTGCAAGCAGGCACTCGAAGACCTGGAGAAGACGTCAGGCCACGACCACCCTGACGTTGCCA
  CCATGCTGAACATCCTGGCACTGGTCTATCGGGATCAGAACAAGTACAAGGAGGCTGCCCACCTGCTCAA
  TGATGCTCTGGCCATCCGGGAGAAAACACTGGGCAAGGACCACCCAGCCGTGGCTGCGACACTAAACAAC
  CTGGCAGTCCTGTATGGCAAGAGGGGCAAGTACAAGGAGGCTGAGCCATTGTGCAAGCGGGCACTGGAGA
  TCCGGGAGAAGGTCCTGGGCAAGTTTCACCCAGATGTGGCCAAGCAGCTCAGCAACCTGGCCCTGCTGTG
  CCAGAACCAGGGCAAAGCTGAGGAGGTGGAATATTACTATCGGCGGGCACTGGAGATCTATGCTACACGC
  CTCGGGCCCGATGACCCCAATGTGGCCAAGACCAAGAACAACCTGGCTTCCTGCTACCTGAAGCAGGGCA
  AGTACCAGGATGCGGAGACCTTGTACAAGGAGATCCTCACCCGCGCTCATGAGAAAGAGTTTGGCTCTGT
  CAATGGGGACAACAAGCCCATCTGGATGCACGCAGAGGAGCGGGAGGAAAGCAAGGATAAGCGCCGGGAC
  AGCGCCCCCTATGGGGAATACGGCAGCTGGTACAAGGCCTGTAAAGTAGACAGCCCCACAGTCAACACCA
  CCCTGCGCAGCTTGGGGGCCCTATACCGGCGCCAGGGCAAGCTGGAAGCCGCGCACACACTAGAGGACTG
  TGCCAGCCGTAACCGCAAGCAGGGTTTGGACCCCGCAAGCCAGACCAAGGTGGTAGAACTGCTGAAAGAT
  GGCAGTGGCAGGCGGGGAGACCGCCGCAGCAGCCGAGACATGGCTGGGGGTGCCGGGCCTCGGTCTGAGT
  CTGACCTCGAGGACGTGGGACCTACAGCTGAGTGGAATGGGGATGGCAGTGGCTCCTTGAGGCGCAGCGG
  TTCCTTTGGGAAACTCCGGGATGCCCTGAGGCGCAGCAGTGAGATGCTGGTAAAGAAGCTGCAGGGGGGC
  ACCCCCCAGGAGCCCCCTAACCCCAGGATGAAGCGGGCCAGTTCCCTCAACTTCCTCAACAAGAGCGTGG
  AAGAGCCGACCCAGCCTGGAGGCACAGGTCTCTCTGACAGCCGCACTCTCAGCTCCAGCTCCATGGACCT
  CTCCCGACGAAGCTCCCTGGTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225936 representing NM_001134774
  Red=Cloning site Green=Tags(s)
  
  MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCILLRRSLEAIEL
  GLGEAQEEKGDVPKDTLDDLFPNEDEQSPAPSPGGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVA
  VPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNN
  LAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATR
  LGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREESKDKRRD
  SAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRNRKQGLDPASQTKVVELLKD
  GSGRRGDRRSSRDMAGGAGPRSESDLEDVGPTAEWNGDGSGSLRRSGSFGKLRDALRRSSEMLVKKLQGG
  TPQEPPNPRMKRASSLNFLNKSVEEPTQPGGTGLSDSRTLSSSSMDLSRRSSLVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134774
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001134774.1, NP_001128246.1
• RefSeq ORF: 1638
• Locus ID: 64837
• Protein Families: Druggable Genome
• MW: 59.9 kDa
• Gene Summary: The protein encoded by this gene is a light chain of kinesin, a molecular motor responsible for moving vesicles and organelles along microtubules. Defects in this gene are a cause of spastic paraplegia, optic atrophy, and neuropathy (SPOAN) syndrome. [provided by RefSeq, Mar 2016]