STXBP2 (NM_001127396) Human Tagged ORF Clone

CAT#: RC225996

STXBP2 (Myc-DDK-tagged)-Human syntaxin binding protein 2 (STXBP2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STXBP2
• Synonyms: FHL5; Hunc18b; MUNC18-2; pp10122; UNC18-2; UNC18B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225996 representing NM_001127396
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCTCGGGGCTGAAGGCGGTGGTGGGGGAAAAAATTCTGAGCGGAGTTATTCGGAGTGTCAAGA
  AGGATGGGGAGTGGAAGGTGCTTATCATGGATCACCCAAGCATGCGCATCTTGTCTTCCTGCTGCAAAAT
  GTCAGATATCCTGGCTGAGGGCATCACCATTGTTGAAGACATCAACAAACGGCGGGAACCCATTCCCAGT
  CTGGAGGCCATTTATTTGCTGAGCCCCACGGAGAAGGCCCTGATCAAAGACTTCCAGGGGACCCCGACTT
  TCACCTACAAAGCGGCCCATATCTTCTTCACCGACACCTGCCCCGAGCCCCTGTTCAGTGAGCTAGGCCG
  CTCTCGTCTGGCAAAGGTGGTGAAGACGTTGAAGGAGATTCACCTTGCCTTCCTCCCCTACGAGGCCCAG
  GTGTTCTCCCTCGATGCTCCCCACAGCACCTACAACCTCTACTGCCCCTTCCGGGCAGAGGAGCGCACGC
  GGCAGCTCGAGGTGCTGGCCCAGCAGATTGCCACGCTGTGCGCCACCCTGCAGGAGTACCCGGCCATCCG
  CTACCGCAAGGGCCCAGAGGACACAGCCCAGTTGGCCCACGCCGTCCTGGCCAAGCTGAACGCCTTCAAG
  GCAGACACTCCCAGTCTGGGCGAGGGCCCAGAGAAAACCCGCTCCCAGCTGCTGATAATGGACCGGGCAG
  CTGACCCCGTGTCCCCACTACTGCATGAGCTCACGTTCCAGGCCATGGCGTATGATCTGCTGGACATAGA
  GCAGGACACATACAGGTATGAGACCACCGGGCTGAGCGAGGCGCGGGAGAAGGCCGTCTTGCTGGACGAG
  GACGATGACTTGTGGGTGGAGCTTCGCCACATGCATATCGCAGATGTGTCCAAGAAGGTCACGGAGCTCC
  TGAGGACCTTCTGTGAGAGCAAGAGGCTGACCACGGACAAGGCGAACATCAAAGACCTATCCCAGATCCT
  GAAAAAGATGCCGCAGTACCAGAAGGAGCTGAATAAGTATTCTACGCACCTGCATCTAGCAGATGATTGT
  ATGAAGCACTTCAAGGGCTCGGTGGAGAAGCTGTGTAGTGTGGAGCAGGACCTGGCCATGGGCTCCGACG
  CAGAGGGGGAGAAGATCAAGGACTCCATGAAGCTGATCGTTCCGGTGCTGCTGGACGCGGCGGTGCCCGC
  CTACGACAAGATCCGGGTCCTGCTGCTCTACATCCTCCTTCGGAATGGTGTGAGTGAGGAGAACCTGGCC
  AAGCTGATCCAGCATGCCAATGTACAGGCGCACAGCAGCCTCATCCGTAACCTGGAGCAGCTGGGAGGCA
  CTGTCACCAACCCCGGGGGCTCGGGGACCTCCAGCCGGCTGGAGCCGAGAGAACGCATGGAGCCCACCTA
  TCAGCTGTCCCGCTGGACCCCGGTCATCAAGGATGTAATGGAGGACGCCGTGGAGGACCGGCTGGACAGG
  AACCTGTGGCCCTTCGTATCCGACCCCGCCCCCACGGCCAGCTCCCAGGCCGCTGTCAGTGCCCGCTTCG
  GTCACTGGCACAAGAACAAGGCTGGCATAGAAGCCCGGGCGGGCCCCCGGCTCATCGTGTATGTCATGGG
  CGGTGTGGCCATGTCAGAGATGAGGGCCGCCTACGAGGTGACCAGGGCCACCGAGGGCAAGTGGGAGGTG
  CTCATTGGCTCCTCACACATCCTCACCCCGACCCGCTTCCTGGATGACCTGAAGGCACTGGACAAGAAGC
  TGGAGGACATTGCCCTGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225996 representing NM_001127396
  Red=Cloning site Green=Tags(s)
  
  MAPSGLKAVVGEKILSGVIRSVKKDGEWKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPS
  LEAIYLLSPTEKALIKDFQGTPTFTYKAAHIFFTDTCPEPLFSELGRSRLAKVVKTLKEIHLAFLPYEAQ
  VFSLDAPHSTYNLYCPFRAEERTRQLEVLAQQIATLCATLQEYPAIRYRKGPEDTAQLAHAVLAKLNAFK
  ADTPSLGEGPEKTRSQLLIMDRAADPVSPLLHELTFQAMAYDLLDIEQDTYRYETTGLSEAREKAVLLDE
  DDDLWVELRHMHIADVSKKVTELLRTFCESKRLTTDKANIKDLSQILKKMPQYQKELNKYSTHLHLADDC
  MKHFKGSVEKLCSVEQDLAMGSDAEGEKIKDSMKLIVPVLLDAAVPAYDKIRVLLLYILLRNGVSEENLA
  KLIQHANVQAHSSLIRNLEQLGGTVTNPGGSGTSSRLEPRERMEPTYQLSRWTPVIKDVMEDAVEDRLDR
  NLWPFVSDPAPTASSQAAVSARFGHWHKNKAGIEARAGPRLIVYVMGGVAMSEMRAAYEVTRATEGKWEV
  LIGSSHILTPTRFLDDLKALDKKLEDIALP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127396
• ORF Size: 1770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127396.1, NM_001127396.2, NP_001120868.1
• RefSeq Size: 1901 bp
• RefSeq ORF: 1773 bp
• Locus ID: 6813
• Cytogenetics: 19p13.2
• Protein Families: Stem cell - Pluripotency
• MW: 66.1 kDa
• Gene Summary: 'This gene encodes a member of the STXBP/unc-18/SEC1 family. The encoded protein is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2013]'