SPIRE1 (NM_001128627) Human Tagged ORF Clone

CAT#: RC226036

SPIRE1 (Myc-DDK-tagged)-Human spire homolog 1 (Drosophila) (SPIRE1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY426984) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226036 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPIRE1
• Synonyms: Spir-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226036 representing NM_001128627
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACAGAGGTCATTGAATCTTTGGGAATTATTATTTATAAAGCACTGGACTATGGTTTGAAGGAGA
  ATGAAGAAAGGGAATTAAGCCCTCCCCTAGAGCAGCTTATCGATCACATGGCCAACACGGTGGAAGCTGA
  CGGTAGCAATGATGAGGGCTATGAGGCTGCAGAAGAAGGCCTGGGAGATGAAGATGAAAAGAGAAAAATC
  TCAGCTATTCGGTCATATAGAGATGTCATGAAGTTGTGTGCTGCTCATCTCCCTACTGAATCAGATGCAC
  CAAATCATTATCAGGCAGTATGTCGTGCACTGTTTGCAGAAACAATGGAGCTCCATACATTTCTGACCAA
  AATTAAGAGTGCGAAAGAGAATCTTAAGAAGATTCAAGAAATGGAAAAGAGCGATGAATCTAGCACAGAC
  TTGGAAGAGCTGAAAAACGCTGACTGGGCACGATTCTGGGTACAGGTGATGAGGGATTTGAGGAATGGGG
  TAAAACTTAAGAAGGTCCAAGAGCGGCAGTACAACCCTTTGCCCATTGAATATCAGCTCACCCCTTATGA
  GATGTTAATGGATGACATTCGCTGCAAAAGATACACCTTGCGAAAAGTGATGGTGAATGGTGATATTCCC
  CCTCGGTTAAAAAAGAGTGCTCATGAAATCATCCTCGACTTCATCAGATCCAGACCTCCTTTAAATCCAG
  TCTCAGCCAGAAAACTGAAACCAACTCCACCACGGCCACGGAGCCTCCATGAAAGAATATTAGAAGAAAT
  TAAAGCAGAAAGAAAGCTGCGGCCTGTATCACCAGAGGAGATTAGACGTAGCAGATTAGATGTGACTACC
  CCTGAATCTACAAAGAATCTTGTGGAGTCATCTATGGTGAATGGAGGTTTGACATCACAAACAAAAGAAA
  ACGGGTTAAGTACCTCACAGCAGGTGCCTGCACAGCGGAAGAAGCTCCTCAGAGCCCCAACTCTGGCCGA
  ACTGGACAGCTCTGAGTCTGAGGAAGAAACGCTGCACAAGTCGACCAGCAGCAGCAGCGTGTCTCCCTCT
  TTCCCTGAAGAGCCAGTCCTGGAGGCCGTGTCCACAAGGAAGAAGCCTCCAAAATTCCTGCCCATATCAT
  CAACACCCCAGCCAGAGAGACGGCAGCCACCCCAGAGACGACATTCCATTGAAAAGGAAACGCCTACTAA
  CGTGAGGCAGTTCCTGCCGCCTTCCAGGCAGAGTTCCCGCTCTCTTGAGGAATTCTGCTACCCAGTGGAA
  TGCCTCGCTCTTACTGTGGAAGAAGTGATGCATATTCGCCAGGTCCTGGTGAAGGCAGAGCTGGAAAAAT
  ACCAACAGTATAAAGACATCTACACCGCCTTGAAAAAAGGAAAGCTCTGCTTTTGTTGCCGAACCAGGAG
  GTTTTCCTTCTTCACTTGGTCTTATACCTGTCAGTTCTGTAAGAGGCCGGTGTGCTCACAGTGTTGCAAA
  AAGATGCGGCTGCCCTCCAAACCATACTCCACTCTTCCTATCTTTTCATTGGGACCTTCTGCTCTGCAAA
  GAGGGGAAAGTAGTATGAGGTCAGAAAAACCCTCCACTGCCCATCATCGGCCACTTCGGAGCATTGCCAG
  GTTCTCCTCAAAATCTAAGTCTATGGACAAATCAGATGAAGAACTCCAGTTTCCCAAAGAGTTGATGGAG
  GACTGGAGCACCATGGAGGTGTGTGTGGACTGCAAGAAGTTCATTTCGGAAATCATCAGTTCAAGCCGGC
  GCAGTCTGGTGTTGGCCAACAAAAGGGCCCGATTGAAAAGGAAAACGCAGTCTTTCTACATGTCCTCGCC
  AGGCCCCTCGGAGTACTGCCCTTCAGAGAGGACGATCAGTGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226036 representing NM_001128627
  Red=Cloning site Green=Tags(s)
  
  METEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYEAAEEGLGDEDEKRKI
  SAIRSYRDVMKLCAAHLPTESDAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESSTD
  LEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRCKRYTLRKVMVNGDIP
  PRLKKSAHEIILDFIRSRPPLNPVSARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLDVTT
  PESTKNLVESSMVNGGLTSQTKENGLSTSQQVPAQRKKLLRAPTLAELDSSESEEETLHKSTSSSSVSPS
  FPEEPVLEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLEEFCYPVE
  CLALTVEEVMHIRQVLVKAELEKYQQYKDIYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCK
  KMRLPSKPYSTLPIFSLGPSALQRGESSMRSEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELME
  DWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128627
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001128627.1, NP_001122099.1
• RefSeq ORF: 1869
• Locus ID: 56907
• Protein Pathways: Dorso-ventral axis formation
• MW: 71.4 kDa
• Gene Summary: Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]). [supplied by OMIM, Mar 2008]