RTL1 (NM_001134888) Human Tagged ORF Clone

CAT#: RC226388

RTL1 (Myc-DDK-tagged)-Human retrotransposon-like 1 (RTL1)

Product Images

Western blot validation of overexpression lysate (Cat# LY427511) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226388 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RTL1
• Synonyms: HUR1; Mar1; MART1; PEG11; SIRH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226388 representing NM_001134888
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGATAGAACCCTCTGAAGACTCATTTGAGACGATGATGGAGCATAAGAATCCATCATCAAAACAAATGG
  AGTCCTCCGAGGGCTCATCCAACACCACCGAGGCGACGTCGGGCAGTGGAGTGCGGGGAGAGGCAGGGCC
  AGCCAGCGGCCCAGCCCAGGAAAAGAAGGAGCCCCCCAGTGGCCCACTCCAGGAAATGGAAGAGCTGCCC
  ACTGATCTACTCCAAGACATGGAGGAGCCATCCAGTGGCCCACGTAAGGAAATAGAGGATCCACCCAATG
  ACCTACTCCAAGACCTGGAGGAGTCATGCAACGGTTCACACCAGGCAAGGGGGGATCCACTCAGTGGAGC
  ATCTGATAGGATGAAAGAAGCATCAGTCAACCCATCGGGAGCCCGAGAAGAACAAGAGGCTCACACTGAC
  CTGAAGGAATCGGGAAGGGAGGAGACTCCTCAAGAGCAAAACCAGACCGAGCACTCAACCGCAGAACTTA
  TGGCCATGGTGAGGTCCATCATCTCGCTGTACTTCCGAATGCAAGACCTCAAAGAGCAACAGAGAGTAGC
  AGAAGAGATCTTGATCAAAGGGATCAATGCAGGCCAACTGCCCGCCCCAAAGCACTTCTCTGGCGATCGC
  AGAGAATTCCACGAGTTCATCGTACTCTGCCAACTGACCTTACAGAGCTACCCAAGAATGTTCTATAACG
  ACCGTCTGAGAGTTGGCTATGTCATCAATCACCTGTCCGGCTTGGCATTAGAATGGGCCAAAGCTCTACT
  GCAGGAAAACAGCCCCCTGATCGGAGACTTCCCAGCCTTCCTGGAGGCCATGTCCGAAGTGTTTGAGTAC
  CGCCAGGCACTGCGTGTGGCAGAAGAGGCCATGTTCACCATCAGGCAGGGCGGCCGCTCTGCCACTGAGT
  ACATCGATGAGTTCCAGAGCCTGGTACCCATCTTGGGCTGGCCAGATGAAGTCCTGCAGGCCCACTTGTG
  CCAGGGGCTCAACGAGGAGATCAGGCACTATCTATTCCGGGTCCCTCAGCCGGATTCCCTAGACAGTCTG
  ATTGTGCTCATCCTGCAAATAGAAGAGAAGCTGGCAGAGAGAAGAGCTATGCTCAGGCTGCCCCCCGAGG
  CCCGCCCCCGGAACCTGACCTGGATCGACTCACCTGCTCCAGAGAGGTGGATGGTCAGCAGCTGGTTGCC
  CAGCGAAGTCCATCCGGACATCAATCGCGCCCACCTCTTCCTGCTGCTCATGGTGAGAGTGAACCCCTAC
  CACAGCGTCGCGGTCCAGGCCCTGGTGGATTCGGGAGCTGACGGCAACTTCATGGATGAGAAGTTCGCCC
  AAGAGCACTACGTCGAGCTCTACGAGAAGCCGTACCCACAGCCGGTCCAATCCGTGGACGGCTCGCTGAT
  TGGCAACGAGCCTGTCTGGCTCTACACGGAGCCCCTGGTGTGTATCCACCAGAACCACCAGGAGTCCATC
  GAATTTGACATCGTACCTTCACCGAACTTCTCTGTGGTCCTAGGCATCCGCTGGCTCCGAGTCCACGCCC
  CCGAAGTCGACTGGATCAAAGGCCGCTGCACCTTCCACTCTCCCTACTGCCTGAAGAACTGCTTCCGCCC
  GCCCCCGCCATGCATTGCCCTAGAGAGGCACGGCATGAGCCTGCTACCCGGACTGCCACACCCATACTCA
  GACCTGGCCGACGTGTTTAACCCGAAGGAAGCAGATGATGAGACTTCCGACCAGCCAAGCTCAGACGGAT
  CCGATGATCTTTCTGAATCAGAGCCCTCTGAGCTTCAGCAGGCTGGAGACAGTGATCACAGCGAGACCTT
  TTACGAGTGTCCCTCCACCGCGCCTTGGGAACCTGTGGGTGCCAGGATGCAAGAAAGAGCCAGGCTACAG
  GAGGAATACTGGGACCTGCAGGACATGCTGACCAACAGACAGGACTACATACAGATGATTCCGGAACTGT
  TTGACCAGTTACACGGAGCCGAGTGGTTCACAAAACTGGAGCTGCGTGGGACCATTGTGGAGGAAAGCGT
  GAACGGGCACCGCACCGAAGATGTGTGGAAAGCAGCGTTTGGTTTGGAGCTTGAAGAGATGAAGAGCTAC
  CAGCCGTTTGCGCTCTCCCCAGACCCTATCATACCTCAGAACGTGATTCACTTCATCCTAAAGGACATGC
  TAGGGTTCTTTGTGCTTTCTTATGGCCAGGAAGTCCTGATCTACTCAATGAGTCAGGAGGAGCACCTCCA
  CCACGTCCGCCAAGTCCTGGTCCGCTTCCGCCATCACAACGTCTACTGCTCCCTGGACAAGAGCCAGTTC
  CACCGCCAAACCGTGGAATTCCTGGGCTTCGTCGTCACCCCCAAAGGGGTGAAACTGAACAAGAACGTCA
  TGACCATCATAACAGGGTACCCTACCCCTGGCTCCAAGCTATCTCTGCGAAACTTCATCGAATTCGTCTT
  CCCCTACCGCCACTTCGTGGAGCGCTTCAGCATCATCGCAGAGCCCCTGGTGCGGCAGCTGCTGAGCTCC
  TACCAGTTCTACTGGGGAGTCGAGGAGCAAGAGGCCTTCGAGTGCCTGAAGAGGGCTTTCCGCAAGGCGC
  CTCTCCTCCACCACCCCAAGCCCCAGAACCCATTCTACTTGGAAACCGGCGTCACCGGCACGGCCCTGCA
  CGCCTCCCTGATCCAAATCGACGACCAAACCGGCAAGAGAGCCTGCTGCGCTTTCTACTCCCGCAACATC
  TCCCCTATCGAGGTTGAGTACTCTCAAGCGGAGATGAAGATTCTTCCAATACGGGCTGCCTTCATGGTGT
  GGTGCCGCTACCTGGAGAACACCGAGGAGCCCATCATGATCCTTCTCAACACAGAGGATCTAGCCTCTCT
  GAATAATGACAGGCTCACCGTACTTCTCCCCGGGCATTGGGTCTTCTTCTTCTCCCACTTCAACTTTGAC
  GTCATGGAGCTGCCAGAACAAGACGGCGGCCGAGCTCTGCCACCTGTGAGAAACCTCCGGTGGAGGAGAG
  CCTTCCAGAGGAACACTGCCGCCAGGCAAACCCTGCTGCTGGCCTCAAGGGGATTCCCCAGGGATCCATC
  GACGGAATCCGGGGAAGAAGAGAATGAAGAACAGGATGAGCTCAATGAACAGATCCTACGGCAAGAGCTG
  CTGGCCATGATACCCATCGACCAGATCCTCAACAGCTTCCTCGCCCACTTCAGCATGGCCCAGATCAGGG
  CCGTCATTCTGCACTTCTTCCGAGGCCTCCTGTACTGGAAGAACACCCTAGCCCTGGCAGCCATCCTCGT
  GCTACTGCGCGTGAGGCAATGCCTCTCGCTGCGGCCGGCACCCGCCATGCGGGTGGCTCGGCCCCAGCCC
  CAGCGCTCCCTACGACTCATCCTGGATTCGTCCCTCATCGCAGGCAGCAGCATCACGACAGCCATCACCC
  AGCTGCTCACCCAGATGCCCGCTCTCGTAGGTGCAAACACCATCCCAGCCCAGGAGCTGGCTGAGCTGTT
  CCTGGGCCCTGGCCGCTGGCAGCGAAATGCTCTGCACTCCCAGGCCCACCGAGGCCTGCAGTTCACCCCT
  GGCTTCTGGCTGACGCTGTGTGAGTTCTTCGGTGTCAGAGTCACCCCCCAGGAGGGCCACCTCCCTGCCC
  TGCGTCAGAACCGCTACCTGGAGCTGCACGTCGTTGGTGATGAGGATGTCGTCTTGCGAGAAGCCCTGCA
  AGACGACCTGCAACGTTACCGTCAGTGTGGCCTGCATGACGGCCTGCAAGACACCTCGCAGGACAAGCAG
  GACAACGACGTGCAGGAGGCCCCACCCAGCCACACAGCAGCCACCCACCCACCCCGCCCACGCCACCTGA
  TGGATCCCCAGGTCCTGGAATTCCTTGGTAGCCGCCTGCTCCACATCCACAGTGCAGATGGCCAGCTGCA
  CCTGCTCAGCCGGGAGCAGGCAGCCAGGGCCCTGAGCCAGTTCCTGACCCTGATCTACAGGCGGGCCCTG
  CCCATCCCCGCCTGGGAGAGCCAGCCCAGGGAGCAGGCAAGGCTAGAAGAGCTGCCTGATGAGGACGAAG
  ATGCTAACCTCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226388 representing NM_001134888
  Red=Cloning site Green=Tags(s)
  
  MIEPSEDSFETMMEHKNPSSKQMESSEGSSNTTEATSGSGVRGEAGPASGPAQEKKEPPSGPLQEMEELP
  TDLLQDMEEPSSGPRKEIEDPPNDLLQDLEESCNGSHQARGDPLSGASDRMKEASVNPSGAREEQEAHTD
  LKESGREETPQEQNQTEHSTAELMAMVRSIISLYFRMQDLKEQQRVAEEILIKGINAGQLPAPKHFSGDR
  REFHEFIVLCQLTLQSYPRMFYNDRLRVGYVINHLSGLALEWAKALLQENSPLIGDFPAFLEAMSEVFEY
  RQALRVAEEAMFTIRQGGRSATEYIDEFQSLVPILGWPDEVLQAHLCQGLNEEIRHYLFRVPQPDSLDSL
  IVLILQIEEKLAERRAMLRLPPEARPRNLTWIDSPAPERWMVSSWLPSEVHPDINRAHLFLLLMVRVNPY
  HSVAVQALVDSGADGNFMDEKFAQEHYVELYEKPYPQPVQSVDGSLIGNEPVWLYTEPLVCIHQNHQESI
  EFDIVPSPNFSVVLGIRWLRVHAPEVDWIKGRCTFHSPYCLKNCFRPPPPCIALERHGMSLLPGLPHPYS
  DLADVFNPKEADDETSDQPSSDGSDDLSESEPSELQQAGDSDHSETFYECPSTAPWEPVGARMQERARLQ
  EEYWDLQDMLTNRQDYIQMIPELFDQLHGAEWFTKLELRGTIVEESVNGHRTEDVWKAAFGLELEEMKSY
  QPFALSPDPIIPQNVIHFILKDMLGFFVLSYGQEVLIYSMSQEEHLHHVRQVLVRFRHHNVYCSLDKSQF
  HRQTVEFLGFVVTPKGVKLNKNVMTIITGYPTPGSKLSLRNFIEFVFPYRHFVERFSIIAEPLVRQLLSS
  YQFYWGVEEQEAFECLKRAFRKAPLLHHPKPQNPFYLETGVTGTALHASLIQIDDQTGKRACCAFYSRNI
  SPIEVEYSQAEMKILPIRAAFMVWCRYLENTEEPIMILLNTEDLASLNNDRLTVLLPGHWVFFFSHFNFD
  VMELPEQDGGRALPPVRNLRWRRAFQRNTAARQTLLLASRGFPRDPSTESGEEENEEQDELNEQILRQEL
  LAMIPIDQILNSFLAHFSMAQIRAVILHFFRGLLYWKNTLALAAILVLLRVRQCLSLRPAPAMRVARPQP
  QRSLRLILDSSLIAGSSITTAITQLLTQMPALVGANTIPAQELAELFLGPGRWQRNALHSQAHRGLQFTP
  GFWLTLCEFFGVRVTPQEGHLPALRQNRYLELHVVGDEDVVLREALQDDLQRYRQCGLHDGLQDTSQDKQ
  DNDVQEAPPSHTAATHPPRPRHLMDPQVLEFLGSRLLHIHSADGQLHLLSREQAARALSQFLTLIYRRAL
  PIPAWESQPREQARLEELPDEDEDANLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134888
• ORF Size: 4074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001134888.1, NM_001134888.2, NP_001128360.1
• RefSeq ORF: 4077
• Locus ID: 388015
• MW: 154.9 kDa
• Gene Summary: This gene is a retrotransposon-derived, paternally expressed imprinted gene that is highly expressed at the late fetal stage in both the fetus and placenta. It has an overlapping maternally expressed antisense transcript, which contains several microRNAs targeting the transcripts of this gene through an RNA interference (RNAi) mechanism. This gene is essential for maintenance of the fetal capillaries. [provided by RefSeq, Jul 2009]