DNMT1 (NM_001130823) Human Tagged ORF Clone

CAT#: RC226414L4

Lenti-ORF clone of DNMT1 (mGFP-tagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: DNMT1
• Synonyms: ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC226414).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_001130823
• ORF Size: 4896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130823.1, NP_001124295.1
• RefSeq ORF: 4899 bp
• Locus ID: 1786
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• MW: 184.6 kDa
• Gene Summary: 'This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]'