Mortality Factor 4 like 2 (MORF4L2) (NM_001142418) Human Tagged ORF Clone

CAT#: RC226523

MORF4L2 (Myc-DDK-tagged)-Human mortality factor 4 like 2 (MORF4L2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY428080) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226523 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MORF4L2 protein (Cat# TP326523). The protein was produced from HEK293T cells transfected with MORF4L2 cDNA clone (Cat# RC226523) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MORF4L2
• Synonyms: MORFL2; MRGX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226523 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCCAGAAAGCAGGGTTCTCAACCTCGTGGACAGCAATCTGCAGAAGAAGAGAACTTCAAAAAAC
  CAACTAGAAGCAACATGCAGAGAAGTAAAATGAGAGGGGCCTCCTCAGGAAAGAAGACAGCTGGTCCACA
  GCAGAAAAATCTTGAACCAGCTCTCCCAGGAAGATGGGGTGGTCGCTCTGCAGAGAACCCCCCTTCAGGA
  TCCGTGAGGAAGACCAGAAAGAACAAGCAGAAGACTCCTGGAAACGGAGATGGTGGCAGTACCAGCGAAG
  CACCTCAGCCCCCTCGGAAGAAAAGGGCCCGGGCAGACCCCACTGTTGAAAGTGAGGAGGCGTTTAAGAA
  TAGAATGGAGGTTAAAGTGAAGATTCCTGAAGAATTAAAACCATGGCTTGTTGAGGACTGGGACTTAGTT
  ACCAGGCAGAAGCAGCTGTTTCAACTCCCTGCCAAGAAAAATGTAGATGCAATTCTGGAGGAGTATGCAA
  ATTGCAAGAAATCGCAGGGAAATGTTGATAATAAGGAATATGCGGTTAATGAAGTTGTGGCAGGAATAAA
  AGAATATTTCAATGTGATGTTGGGCACTCAGCTGCTCTACAAATTTGAGAGGCCCCAGTATGCTGAAATC
  CTCTTGGCTCACCCTGATGCTCCAATGTCCCAGGTTTATGGAGCACCACACCTACTGAGATTATTTGTAA
  GAATTGGAGCAATGTTGGCCTATACGCCCCTTGATGAGAAAAGCCTTGCATTATTGTTGGGCTATTTGCA
  TGATTTCCTAAAATATCTGGCAAAGAATTCTGCATCTCTCTTTACTGCCAGTGATTACAAAGTGGCTTCT
  GCTGAGTACCACCGCAAAGCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226523 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSRKQGSQPRGQQSAEEENFKKPTRSNMQRSKMRGASSGKKTAGPQQKNLEPALPGRWGGRSAENPPSG
  SVRKTRKNKQKTPGNGDGGSTSEAPQPPRKKRARADPTVESEEAFKNRMEVKVKIPEELKPWLVEDWDLV
  TRQKQLFQLPAKKNVDAILEEYANCKKSQGNVDNKEYAVNEVVAGIKEYFNVMLGTQLLYKFERPQYAEI
  LLAHPDAPMSQVYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLGYLHDFLKYLAKNSASLFTASDYKVAS
  AEYHRKAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142418
• ORF Size: 864 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142418.1, NP_001135890.1
• RefSeq Size: 2047
• RefSeq ORF: 867
• Locus ID: 9643
• Protein Families: Transcription Factors
• MW: 32.3 kDa
• Gene Summary: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones. [UniProtKB/Swiss-Prot Function]