DCTN4 (NM_001135643) Human Tagged ORF Clone

CAT#: RC226530

DCTN4 (Myc-DDK-tagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427644) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226530 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCTN4
• Synonyms: DYN4; P62
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226530 representing NM_001135643
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCTTGCTGCAGTCGGACCGGGTTCTCTATCTAGTCCAGGGAGAAAAGAAGGTTCGGGCCCCGC
  TCTCGCAACTCTACTTCTGCCGCTATTGTAGCGAACTGCGGTCGCTGGAATGTGTGTCTCACGAGGTGGA
  CTCCCATTATTGTCCCAGTTGTTTAGAAAATATGCCATCGGCTGAAGCCAAACTAAAAAAGAATAGATGT
  GCCAATTGTTTTGACTGTCCTGGCTGCATGCACACCCTCTCTACTCGGGCCACGAGCATCTCCACACAGC
  TTCCAGATGACCCAGCCAAGACCACCATGAAGAAAGCCTATTACCTGGCATGTGGATTTTGTCGCTGGAC
  GTCTAGAGATGTGGGCATGGCAGACAAATCTGTAGCTAGTGGCGGTTGGCAGGAACCTGAAAATCCTCAC
  ACACAACGGATGAACAAATTGATTGAATATTACCAGCAGCTTGCTCAGAAAGAGAAGGTTGAGCGAGATC
  GCAAGAAACTGGCACGACGTAGAAACTATATGCCTCTGGCTTTTTCGCAACATACTATTCATGTAGTGGA
  CAAATATGGTCTTGGAACCAGGCTTCAGCGACCACGAGCTGGTGCATCCATCAGTACCCTTGCCGGACTT
  TCCCTTAAAGAAGGAGAGGATCAGAAAGAGATAAAGATTGAGCCAGCTCAGGCTGTGGATGAAGTGGAAC
  CTCTACCTGAAGACTATTATACAAGACCAGTAAATTTAACAGAGGTAACAACCCTTCAGCAGCGTCTGTT
  ACAGCCTGACTTCCAGCCAGTCTGTGCTTCACAGCTCTATCCTCGCCACAAACATCTTCTGATCAAACGG
  TCCCTGCGCTGCCGTAAATGTGAACATAATTTGAGCAAGCCAGAATTTAACCCAACGTCAATCAAATTCA
  AAATCCAGCTGGTCGCTGTCAATTATATTCCAGAAGTGAGAATCATGTCAATTCCCAACCTTCGCTACAT
  GAAGGAGAGCCAGGTCCTCCTGACTCTTACAAATCCAGTTGAGAACCTCACCCATGTGACTCTCTTCGAG
  TGTGAGGAGGGGGACCCTGATGATATCAACAGCACTGCTAAGGTGGTGGTGCCTCCCAAAGAGCTCGTTT
  TAGCTGGCAAGGATGCAGCAGCAGAGTACGATGAGTTGGCAGAACCTCAAGACTTTCAGGACGATCCTGA
  CATTATAGCCTTCAGAAAGGCCAACAAAGTGGGTATTTTCATCAAAGTTACACCACAGCGTGAGGAGGGT
  GAAGTGACCGTGTGCTTCAAGATGAAGCATGATTTTAAAAACCTGGCAGCCCCCATTCGCCCCATTGAAG
  AAAGTGACCAGGGAACAGAAGTCATCTGGCTCACCCAGCATGTGGAACTTAGCTTGGGCCCACTTCTTCC
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226530 representing NM_001135643
  Red=Cloning site Green=Tags(s)
  
  MASLLQSDRVLYLVQGEKKVRAPLSQLYFCRYCSELRSLECVSHEVDSHYCPSCLENMPSAEAKLKKNRC
  ANCFDCPGCMHTLSTRATSISTQLPDDPAKTTMKKAYYLACGFCRWTSRDVGMADKSVASGGWQEPENPH
  TQRMNKLIEYYQQLAQKEKVERDRKKLARRRNYMPLAFSQHTIHVVDKYGLGTRLQRPRAGASISTLAGL
  SLKEGEDQKEIKIEPAQAVDEVEPLPEDYYTRPVNLTEVTTLQQRLLQPDFQPVCASQLYPRHKHLLIKR
  SLRCRKCEHNLSKPEFNPTSIKFKIQLVAVNYIPEVRIMSIPNLRYMKESQVLLTLTNPVENLTHVTLFE
  CEEGDPDDINSTAKVVVPPKELVLAGKDAAAEYDELAEPQDFQDDPDIIAFRKANKVGIFIKVTPQREEG
  EVTVCFKMKHDFKNLAAPIRPIEESDQGTEVIWLTQHVELSLGPLLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135643
• ORF Size: 1401 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001135643.1, NP_001129115.1
• RefSeq ORF: 1404
• Locus ID: 51164
• Protein Pathways: Huntington's disease
• MW: 53 kDa
• Gene Summary: Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton. [UniProtKB/Swiss-Prot Function]