NCOA4 (NM_001145262) Human Tagged ORF Clone

CAT#: RC226691

NCOA4 (Myc-DDK-tagged)-Human nuclear receptor coactivator 4 (NCOA4), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY428771) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226707 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NCOA4 protein (Cat# TP326691). The protein was produced from HEK293T cells transfected with NCOA4 cDNA clone (Cat# RC226691) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NCOA4
• Synonyms: ARA70; ELE1; PTC3; RFG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226691 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATACCTTCCAAGACCAGAGTGGCAGCTCCAGTAATAGAGAACCCCTTTTGAGGTGTAGTGATGCAC
  GGAGGGACTTGGAGCTTGCTATTGGTGGAGTTCTCCGGGCTGAACAGCAAATTAAAGATAACTTGCGAGA
  GGTCAAAGCTCAGATTCACAGTTGCATAAGCCGTCACCTGGAATGTCTTAGAAGCCGTGAGGTATGGCTG
  TATGAACAGGTGGACCTTATTTATCAGCTTAAAGAGGAGACACTTCAACAGCAGGCTCAGCAGCTCTACT
  CGTTATTGGGCCAGTTCAATTGTCTTACTCATCAACTGGAGTGTACCCAAAACAAAGATCTAGCCAATCA
  AGTCTCTGTGTGCCTGGAGAGACTGGGCAGTTTGACCCTTAAGCCTGAAGATTCAACTGTCCTGCTCTTT
  GAAGCTGACACAATTACTCTGCGCCAGACCATCACCACATTTGGGTCTCTCAAAACCATTCAAATTCCTG
  AGCACTTGATGGCTCATGCTAGTTCAGCAAATATTGGGCCCTTCCTGGAGAAGAGAGGCTGTATCTCCAT
  GCCAGAGCAGAAGTCAGCATCCGGTATTGTAGCTGTCCCTTTCAGCGAATGGCTCCTTGGAAGCAAACCT
  GCCAGTGGTTATCAAGCTCCTTACATACCCAGCACCGACCCCCAGGACTGGCTTACCCAAAAGCAGACCT
  TGGAGAACAGTCAGACTTCTTCCAGAGCCTGCAATTTCTTCAATAATGTCGGGGGAAACCTAAAGGGCTT
  AGAAAACTGGCTCCTCAAGAGTGAAAAATCAAGTTATCAAAAGTGTAACAGCCATTCCACTACTAGTTCT
  TTCTCCATTGAAATGGAAAAGGTTGGAGATCAAGAGCTTCCTGATCAAGATGAGATGGACCTATCAGATT
  GGCTAGTGACTCCCCAGGAATCCCATAAGCTGCGGAAGCCTGAGAATGGCAGTCGTGAAACCAGTGAGAA
  GTTTAAGCTCTTATTCCAGTCCTATAATGTGAATGATTGGCTTGTCAAGACTGACTCCTGTACCAACTGT
  CAGGGAAACCAGCCCAAAGGTGTGGAGATTGAAAACCTGGGCAATCTGAAGTGCCTGAATGACCACTTGG
  AGGCCAAGAAACCATTGTCCACCCCCAGCATGGTTACAGAGGATTGGCTTGTCCAGAACCATCAGGACCC
  ATGTAAGGTAGAGGAGGTGTGCAGAGCCAATGAGCCCTGCACAAGCTTTGCAGAGTGTGTGTGTGATGAG
  AATTGTGAGAAGGAGGCTCTGTATAAGTGGCTTCTGAAGAAAGAAGGAAAGGATAAAAATGGGATGCCTG
  TGGAACCCAAACCTGAGCCTGAGAAGCATAAAGATTCCCTGAATATGTGGCTCTGTCCTAGAAAAGAAGT
  AATAGAACAAACTAAAGCACCAAAGGCAATGACTCCTTCTAGAATTGCTGATTCCTTCCAAGTCATAAAG
  AACAGCCCCTTGTCGGAGTGGCTTATCAGGCCCCCATACAAAGAAGGAAGTCCCAAGGAAGTGCCTGGTA
  CTGAAGACAGAGCTGGCAAACAGAAGTTTAAAAGCCCCATGAATACTTCCTGGTGTTCCTTTAACACAGC
  TGACTGGGTCCTGCCAGGAAAGAAGATGGGCAACCTCAGCCAGTTATCTTCTGGAGAAGACAAGTGGCTG
  CTTCGAAAGAAGGCCCAGGAAGTATTACTTAATTCACCTCTACAGGAGGAACATAACTTCCCCCCAGACC
  ATTATGGCCTCCCTGCAGTTTGTGATCTCTTTGCCTGTATGCAGCTTAAAGTTGATAAAGAGAAGTGGTT
  ATATCGAACTCCTCTACAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226691 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNTFQDQSGSSSNREPLLRCSDARRDLELAIGGVLRAEQQIKDNLREVKAQIHSCISRHLECLRSREVWL
  YEQVDLIYQLKEETLQQQAQQLYSLLGQFNCLTHQLECTQNKDLANQVSVCLERLGSLTLKPEDSTVLLF
  EADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKRGCISMPEQKSASGIVAVPFSEWLLGSKP
  ASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSS
  FSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNC
  QGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDE
  NCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIK
  NSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWL
  LRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKWLYRTPLQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145262
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145262.1, NP_001138734.1
• RefSeq Size: 3588 bp
• RefSeq ORF: 1845 bp
• Locus ID: 8031
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Thyroid cancer
• MW: 69.7 kDa
• Gene Summary: This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009]