BHLHB9 (NM_001142527) Human Tagged ORF Clone

CAT#: RC226822

BHLHB9 (Myc-DDK-tagged)-Human basic helix-loop-helix domain containing, class B, 9 (BHLHB9), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BHLHB9
• Synonyms: GASP3; p60TRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226822 representing NM_001142527
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGACTAAGAATAAGACAAGAGCCCAGGCCAAAACTGAAAAAAAGGCTGCTATACAAGCTAAAG
  CTGGAGCAGAGAGGGAGGCTACTGGTGTTGTTAGGCCTGTAGCCAAGACCAGGGCCAAAGCAAAAGCCAA
  GACAGGGTCTAAGACAGATGCAGTAGCAGAGATGAAGGCAGTGTCTAAGAACAAGGTTGTTGCTGAGACG
  AAGGAAGGAGCTCTGTCAGAGCCTAAGACTCTGGGCAAAGCCATGGGAGATTTCACTCCCAAGGCTGGGA
  ATGAGTCCACCAGCTCCACATGTAAAAATGAGGCTGGTACTGATGCCTGGTTCTGGGCTGGGGAAGAGGC
  CACTATCAATTCCTGGTTCTGGAATGGAGAAGAGGCTGGTAATAGTTTCAGCACTAAGAATGATAAACCT
  GAAATTGGTGCCCAGGTCTGTGCTGAGGAGTTGGAACCTGCGGCTGGGGCCGATTGCAAACCTAGGTCAG
  GGGCTGAGGAGGAGGAGGAAGAGAATGTTATTGGGAACTGGTTTTGGGAAGGAGATGATACTAGTTTTGA
  CCCTAATCCTAAACCTGTGAGCAGGATAGTTAAGCCTCAGCCTGTGTATGAAATTAATGAAAAAAATAGG
  CCCAAGGACTGGTCTGAGGTAACTATCTGGCCCAATGCCCCTGCTGTAACTCCAGCTGTGTTAGGATTTA
  GATCACAGGCACCATCTGAGGCAAGCCCTCCTTCATATATTGTTCTGGCCTCCGCTGAAGAAAATGCCTG
  TTCTTTGCCTGTGGCAACAGCTTGCCGCCCTTCTAGGAACACTCGCTCATGCTCACAGCCTATCCCTGAG
  TGTCGTTTTGATTCTGACCCCTGCATCCAGACCATAGATGAGATTAGACGTCAAATCAGGATCAGGGAGG
  TAAATGGGATTAAGCCATTTGCTTGTCCTTGCAAAATGGAATGCTATATGGATTCTGAGGAATTTGAAAA
  ACTTGTTAGCTTACTTAAGTCAACTACTGATCCTCTTATTCATAAAATAGCACGGATTGCAATGGGTGTC
  CATAATGTTCACCCATTTGCCCAAGAGTTTATTAACGAAGTAGGTGTAGTGACACTTATTGAAAGCTTGC
  TCAGTTTTCCTTCCCCTGAAATGAGAAAAAAGACTGTAATTACTCTGAATCCTCCTTCTGGGGATGAAAG
  ACAACGCAAAATTGAATTACATGTTAAGCATATGTGTAAAGAAACCATGTCATTTCCTTTGAACTCACCG
  GGACAGCAATCTGGATTAAAGATACTAGGACAACTGACTACTGATTTTGTCCATCACTACATTGTTGCCA
  ATTACTTTTCAGAGCTTTTCCATTTGCTGTCCTCAGGAAATTGCAAAACCAGAAATCTTGTTTTGAAACT
  ACTTTTAAATATGTCTGAAAATCCAACTGCAGCCAGAGACATGATCAATATGAAGGCATTGGCAGCATTA
  AAACTCATCTTTAACCAGAAAGAGGCAAAAGCCAATCTTGTTAGTGGTGTGGCCATATTTATTAACATAA
  AGGAGCATATCAGAAAAGGCTCAATTGTAGTTGTTGATCACTTGAGTTATAATACACTCATGGCCATTTT
  CAGGGAAGTTAAAGAGATTATTGAAACAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226822 representing NM_001142527
  Red=Cloning site Green=Tags(s)
  
  MAGTKNKTRAQAKTEKKAAIQAKAGAEREATGVVRPVAKTRAKAKAKTGSKTDAVAEMKAVSKNKVVAET
  KEGALSEPKTLGKAMGDFTPKAGNESTSSTCKNEAGTDAWFWAGEEATINSWFWNGEEAGNSFSTKNDKP
  EIGAQVCAEELEPAAGADCKPRSGAEEEEEENVIGNWFWEGDDTSFDPNPKPVSRIVKPQPVYEINEKNR
  PKDWSEVTIWPNAPAVTPAVLGFRSQAPSEASPPSYIVLASAEENACSLPVATACRPSRNTRSCSQPIPE
  CRFDSDPCIQTIDEIRRQIRIREVNGIKPFACPCKMECYMDSEEFEKLVSLLKSTTDPLIHKIARIAMGV
  HNVHPFAQEFINEVGVVTLIESLLSFPSPEMRKKTVITLNPPSGDERQRKIELHVKHMCKETMSFPLNSP
  GQQSGLKILGQLTTDFVHHYIVANYFSELFHLLSSGNCKTRNLVLKLLLNMSENPTAARDMINMKALAAL
  KLIFNQKEAKANLVSGVAIFINIKEHIRKGSIVVVDHLSYNTLMAIFREVKEIIETM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142527
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142527.1, NP_001135999.1
• RefSeq ORF: 1644 bp
• Locus ID: 80823
• MW: 60.1 kDa
• Gene Summary: This gene is a member of a gene family which encodes proteins with a basic helix-loop-helix domain. Other members of this gene family encode proteins which function as transcription factors, either enhancing or inhibiting transcription depending on the activity of other DNA binding proteins. The coding region of this gene is located entirely within the terminal exon. The encoded protein may be involved in the survival of neurons (PMID: 15034937). Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2011]